Biocompatibility and bioactivity of calcium silicate-based endodontic sealers in human dental pulp cells

Abstract

Mineral Trioxide Aggregate (MTA) is a calcium silicate-based material. New sealers have been developed based on calcium silicate as MTA Fillapex and MTA Plus. Objective The aim of this study was to evaluate biocompatibility and bioactivity of these two calcium silicate-based sealers in culture of human dental pulp cells (hDPCs).Material and Methods The cells were isolated from third molars extracted from a 16-year-old patient. Pulp tissue was sectioned into fragments with approximately 1 mm3 and kept in supplemented medium to obtain hDPCs adherent cultures. Cell characterization assays were performed to prove the osteogenic potential. The evaluated materials were: MTA Plus (MTAP); MTA Fillapex (MTAF) and FillCanal (FC). Biocompatibility was evaluated with MTT and Neutral Red (NR) assays, after hDPCs exposure for 24 h to different dilutions of each sealer extract (1:2, 1:3 and 1:4). Unexposed cells were the positive control (CT). Bioactivity was assessed by alkaline phosphatase (ALP) enzymatic assay in cells exposed for one and three days to sealer extracts (1:4 dilution). All data were analyzed by ANOVA and Tukey post-test (p≤0.05%).Results MTT and NR results showed suitable cell viability rates for MTAP at all dilutions (90-135%). Cells exposed to MTAF and FC (1:2 and 1:4 dilutions) showed significant low viability rate when compared to CT in MTT. The NR results demonstrated cell viability for all materials tested. In MTAP group, the cells ALP activity was similar to CT in one and three days of exposure to the material. MTAF and FC groups demonstrated a decrease in ALP activity when compared to CT at both periods of cell exposure.Conclusions The hDPCs were suitable for the evaluation of new endodontic materials in vitro. MTAP may be considered a promising material for endodontic treatments.

Zinc-Containing Hydroxyapatite Enhances Cold-Light-Activated Tooth Bleaching Treatment In Vitro

“…The difference is considered to be caused by the constant Ca/P ratio in the synthesis. According to the cell parameter results obtained by Rietveld refinement of the XRD data (Table 1), the lattice cell parameters of Zn-HA continue to decrease with substitution of Zn 2+ ions increasingly getting into the apatite structure, following the trend described by other works [8]. It makes sense that both the a - and c -axes decreased with Zn substitution since the size of Zn 2+ ions (0.074 nm) is smaller than that of Ca 2+ (0.099 nm).…”

“…Previous studies have reported that Zn-HA has favourable effects on reducing inflammation and improving monocyte chemotaxis [11]. Additionally, zinc ion promotes the anabolic effects of insulin-like growth factor-I (IGF-I) in MC3T3 osteoblastic cells by increasing both the alkaline phosphatase activity and protein concentration [8, 12], due to the fact that the zinc binding protein metallothionein plays an important role in the regulation of cell cycle and differentiation [13]. Not only can zinc promote osteoblast proliferation and differentiation, but it has also shown an inhibitory effect on osteoclastic bone resorption [14].…”

Effect of root canal sealers on human periodontal ligament fibroblast viability: ex vivo study

“…In the experiment, MTT assay was used, which involves the measurement of mitochondrial activity of succinate dehydrogenase, thus informing about the proper functioning of the cultured cells [6, 15, 17, 19, 24]. Although the technique is simple, it is sufficiently precise and allows for obtaining quick results.…”

Biocompatibility of three new calcium silicate‐based endodontic sealers on human periodontal ligament stem cells

“…, Mestieri et al . , Zhou et al . ), whilst Endoseal MTA (EndoSeal, Maruchi, Seoul, Korea), a new sealer without resin, had lower cell viability than ProRoot (PMTA; Dentsply, Tulsa, OK, USA) (Lim et al .…”