Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway

Jianru, Y I; MeiLe, L I; Yang, Yan; Zheng, Wei; Yu, Lingxue; Zhao, Zhihe

doi:10.1590/1678-775720150156

Cited by 4 publications

(4 citation statements)

References 32 publications

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“…Nishijima et al [68] reported decreased or unaffected OPG expression in PL cells subjected to compression in a 2D monolayer system. This is in contrast to the findings of Jianru et al [44], who reported that mechanical stimuli cause a regulated and significant increase in OPG expression in 3D culture. The delay in increase in OPG expression could explain post-pressure bone formation, which prevents alveolar bone loss and allows dental movement.…”

Section: Discussioncontrasting

confidence: 99%

“…Thus, 3D systems possibly increase cell viability and system integrity [66]. Jianru et al [44] showed that PL cells grown over a poly-L-glycolic acid-poly-L-lactic acid matrix exhibit significantly decreased OPG/RANKL ratios after 3-6 h under a compressive force, indicating potential induction of osteoclastogenesis. Upon application of a compressive force, OPG expression decreases after 3 h, begins to increase after 6 h, and reaches significantly high levels after 12 h; this regulation of OPG expression indicates suppression of bone resorption.…”

Section: Discussionmentioning

confidence: 99%

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Review of the biological aspects in the periodontal ligament and alveolar bone associated with compression

2020

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Background: Tissue remodeling requires protein degradation through a process directly dependent on metalloproteinases — enzymes capable of degrading the majority of proteins in this system. This process is regulated by some enzyme inhibitors, and balance between enzyme and inhibitor activity is fundamental to tissue integrity and health. Bone resorption and formation, and the molecules involved in these processes, raise questions on possible differences in tissue behavior in the system. Histological studies of tissues surrounding teeth that have been subjected to such forces show the presence of tension (associated with bone formation) and compression, which induce bone resorption. The duration of load application is an important parameterin osteoclastogenesis. The present study aimed to analyze the biological aspects associated with in vitro compression of the PL and alveolar bone. To this end, we reviewed articles describing the biological events that occur in the PL and alveolar bone during load application on this system and the possible influence of culture medium on the expression of molecules involved in response to such an event. Methods: This literature review included articles published between March 2008 and January 2018 describing in vitro laboratory tests and data on load application and its associated biological events in the human PL and alveolar bone, including studies on molecular-expression events and pathways involved in the process. Conclusions: Intermittent compressive forces stimulate bone resorption in orthodontic treatment, while cyclical forces induce cellular apoptosis at lighter loads than do compressive forces. Both 2D and 3D cell-culture systems seem to reproduce the physiology of evaluated tissues and are reliable analytical models for research purposes.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Review of the biological aspects in the periodontal ligament and alveolar bone associated with compression

2020

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“…It was previously reported that intracellular Ca 2+ could influence CREB via calmodulin-dependent kinase IV (CaMK-IV) 25 . As a result, we further explored the change of CaMK-IV expression.…”

Section: Resultsmentioning

confidence: 99%

Alcohol amplifies ketamine-induced apoptosis in primary cultured cortical neurons and PC12 cells through down-regulating CREB-related signaling pathways

Zuo

Sun

Cui

et al. 2017

Sci Rep

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Recreational use of ketamine (KET) has been increasing worldwide. Previous studies have demonstrated that KET induced neurotoxicity; however, few studies have examined how alcohol (ALC) affects KET-induced neurotoxicity. In light of the fact that some KET abusers combine KET with ALC, the present study was aimed to investigate the effects of ALC on KET-induced neurotoxicity and the underlying mechanism in vitro. Our data revealed that co-treatment with ALC and KET was more detrimental to cell viability than KET single treatment in both PC12 cells and primary cultured rat cortical neurons. Furthermore, ALC exacerbated KET-induced apoptosis characterized by morphological changes and the sub-G1 phase increase, which were mitigated by the pretreatment of CNQX, a known alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA)/kainite (KA) receptor antagonist. In addition, ALC and KET co-treatment led to intracellular Ca2+ overload, down-regulation of p-Akt, p-CREB, PKA, CaMK-IV, Bcl-2 and BDNF expression and up-regulation of cleaved caspase-3 and Bax expression, which can be attenuated by CNQX pretreatment. These results indicate that the potentiation of ALC on KET-induced neurotoxicity was related to the down-regulation of CREB-related pathways. Our present study also indicates that ALC and KET co-abuse might cause serious neurotoxicity which should be conveyed to the public and drew enough attention.

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“…In a study using collagen gel scaffolds, an increase in TNFRSF11B gene expression was observed [ 26 ]. In all studies applying PLGA scaffolds, a decrease in TNFRSF11B secretion was positively correlated with force magnitude and negatively correlated with force duration [ 27 , 28 , 31 , 33 , 43 ]. With one exception [ 28 ], a comparison of TNFSF11 and TNFRSF11B gene expression in the aforementioned studies showed that a rapid down/regulation of TNFRSF11B appears parallel to a rapid upregulation of TNFSF11 in 3D WAB in vitro loading.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Weight-Loaded Cell Models for Understanding Mechanodependent Molecular Pathways Involved in Orthodontic Tooth Movement: A Systematic Review

Janjić

Docheva

Trickovic-Janjic

et al. 2018

Stem Cells International

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Cells from the mesenchymal lineage in the dental area, including but not limited to PDL fibroblasts, osteoblasts, and dental stem cells, are exposed to mechanical stress in physiological (e.g., chewing) and nonphysiological/therapeutic (e.g., orthodontic tooth movement) situations. Close and complex interaction of these different cell types results in the physiological and nonphysiological adaptation of these tissues to mechanical stress. Currently, different in vitro loading models are used to investigate the effect of different types of mechanical loading on the stress adaptation of these cell types. We performed a systematic review according to the PRISMA guidelines to identify all studies in the field of dentistry with focus on mechanobiology using in vitro loading models applying uniaxial static compressive force. Only studies reporting on cells from the mesenchymal lineage were considered for inclusion. The results are summarized regarding gene expression in relation to force duration and magnitude, and the most significant signaling pathways they take part in are identified using protein-protein interaction networks.

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Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway

Cited by 4 publications

References 32 publications

Review of the biological aspects in the periodontal ligament and alveolar bone associated with compression

Review of the biological aspects in the periodontal ligament and alveolar bone associated with compression

Alcohol amplifies ketamine-induced apoptosis in primary cultured cortical neurons and PC12 cells through down-regulating CREB-related signaling pathways

In Vitro Weight-Loaded Cell Models for Understanding Mechanodependent Molecular Pathways Involved in Orthodontic Tooth Movement: A Systematic Review

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