Apoptosis of human tongue squamous cell carcinoma cell (CAL-27) induced by Lactobacillus sp. A-2 metabolites

Abstract

ObjectiveTo study the effect of Lactobacillus sp. A-2 metabolites on viability of CAL-27 cells and apoptosis in CAL-27 cells.MethodsLactobacillus sp. A-2 metabolites 1 and 2 (LM1 and LM2) were obtained by culturing Lactobacillus sp. A-2 in reconstituted whey medium and whey-inulin medium; the cultured CAL-27 cells were treated with different concentrations of LM1 and LM2 (0, 3, 6, 12, 24, 48 mg/mL) and assayed by methyl thiazolyltetrazolium (MTT) method; morphological changes of apoptotic cell were observed under fluorescence microscopy by acridine orange (Ao) fluorescent staining; flow cytometry method (FCM) and agarose gel electrophoresis were used to detect the apoptosis of CAL-27 cells treated LM1 and LM2.ResultsThe different concentrations of LM1 and LM2 could restrain the growth of CAL-27 cells, and in a dose-dependent manner; the apoptosis of CAL-27 cells was obviously induced and was time-dependent. ConclusionsViability of CAL-27 cells was inhibited by Lactobacillus sp. A-2 metabolites; Lactobacillus sp. A-2 metabolites could induce CAL-27 cells apoptosis; study on the bioactive compounds in the Lactobacillus sp. A-2 metabolites and their molecular mechanism is in progress.

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“…Metabolites extracted from Lactobacillus sp. used in yoghurt fermentation have been shown to have potential roles in the inhibition of growth and induction of apoptosis in human tongue squamous carcinoma (CAL-27) cells in vitro [40]. The metabolites, from Lactobacillus sp., contain a number of peptides, amino acids, short chain fatty acids, lactic acid, butyric acid, and certain other chemicals, which may play important roles in its anticancer activities.…”

“…The metabolites, from Lactobacillus sp., contain a number of peptides, amino acids, short chain fatty acids, lactic acid, butyric acid, and certain other chemicals, which may play important roles in its anticancer activities. These metabolites induced cytotoxic effects in CAL-27 cells of early apoptotic by 12.8%, while the percentage of the late cells was up to 69.48% [40]. Heat killed cells of Lactobacillus casei ATCC 393 and Lactobacillus acidophilus 606 have been reported to have inhibitory effects against cancer cells [6] by the production of soluble polysaccharides, which inhibit the proliferation of HT29 and HeLa cancer cells.…”

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