Enamel crystals of mice susceptible or resistant to dental fluorosis: an AFM study

Buzalaf, Marília Afonso Rabelo; Barbosa, Célia Marisa Rizzatti; Leite, Aline de Lima; Chang, Soo-Eun; Liu, Junjun; Czajka-Jakubowska, Agata; Clarkson, Brian H.

doi:10.1590/1678-775720130515

Cited by 4 publications

(2 citation statements)

References 24 publications

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“…That is why, depending on the nature of the region, scientists offer various preventive measures [1,2]. M. A. Buzalaf et al [6] believe that despite the causal relationship between fluoride exposure and dental fluorosis, other factors likely account for its severity. There are observations that certain ethnic groups are more susceptible to this disease.…”

Section: Discussionmentioning

confidence: 99%

Morphological features of enamel in fluorosis of different degrees of severity

Marchenko,

Nikolishyna,

Ilenko

et al. 2024

Rep. of Morph.

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The occurrence of dental fluorosis is facilitated by a violation of enamel mineralization caused by fluorides, which enter the human body in excess during its development and formation and have a toxic effect on enamel blasts. However, the molecular mechanisms involved in the pathogenesis of fluorosis are not fully understood. Enamel formation is a complex process involving cell proliferation and differentiation through epithelial-mesenchymal sequential secretion of matrix proteins, tissue-specific transport of ions including calcium and fluoride, and precipitation and alignment of enamel crystals through interactions between organic and inorganic molecules. Understanding the morphological features of enamel changes during fluoride intoxication of the human body in the endemic region allows us to clearly understand the need for a comprehensive solution to this medical and social problem. The aim was to study the morphological features of enamel in fluorosis in residents of the endemic region of Ukraine, in particular the Poltava region. The work examines different groups of teeth (both intact and affected by fluorosis) removed for orthodontic or clinical indications in men and women aged 17 to 40 years. Morphological signs were studied first on native, and later on histochemically stained sections. It was established that the violation of the structure of the enamel layer of the teeth in mild and severe fluorosis is characterized by both partial and complete violation of the movement of the enamel prisms with signs of destruction. Fragmentation and homogeneity throughout the entire thickness were found in some areas of the enamel. When evaluating histochemically stained sections of teeth affected by fluorosis, it was established that dystrophic changes in the enamel structure and accumulation of acidic glycosaminoglycans in the lesions are more characteristic of mild and moderate forms. Under the conditions of a severe form of fluorosis, complete destruction of the prisms, fragmentation of the lamella, homogenization of areas on the entire enamel layer, which is due to the uneven distribution of acidic mucopolysaccharides, have been established. Morphologically and histochemically dystrophic changes in the areas of the affected enamel are confirmed by uneven distribution and accumulation of acidic glycosaminoglycans. An assessment of the effect of fluoride intoxication on the state of tooth enamel was carried out, which will allow to expand the possibilities of preventive measures for related specialists, as well as to create and develop additional treatment methods that will contribute to the improvement of physical and aesthetic indicators of teeth. dental health.

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Section: Discussionmentioning

confidence: 99%

Morphological features of enamel in fluorosis of different degrees of severity

Marchenko,

Nikolishyna,

Ilenko

et al. 2024

Rep. of Morph.

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show abstract

“…The earliest works aimed to inspect the native dental tissue, especially dentin, after acid treatment (Marshall et al, 1993). In Buzalaf et al (2014), the profile of mice teeth enamel crystals as responding to fluorosis was investigated, instead. An interesting comparison of AFM vs. SEM imaging of dentin was made in Kubinek et al (2007), who pointed out the presence of artifacts due to dehydration in SEM.…”

Section: Afm In Dentistrymentioning

confidence: 99%

Dentistry on the Bridge to Nanoscience and Nanotechnology

Salerno

Diaspro

2015

Front. Mater.

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Dentistry is the area of medical sciences that is most resistant to the introduction of the novel methods arisen from the development of nanoscience and nanotechnology in the last 20 years. Without moving on to science-fiction-like views pointing to times far ahead in the future, we show that the available nanoscale devices and processes of current science and technology, partly inherited from the areas of microscopy and microelectronics, have already proven to be useful for research and development in different fields of dental research. To this goal, we review some results obtained in the last few years at our Institute in the area of dental materials and their characterization, which showed successful application of our background in microscopy and nanoengineering.Keywords: dental materials, microscopy, surface roughness, nanofabrication, resin composites, implants THE BROAD SCOPE OF DENTISTRYDentistry is considered to be a branch of medicine, such as orthopedics or physiology or neurology. In fact, while based on common foundations of medicine, dentistry is a large stand-alone area, which has its own grasps to the different fields mentioned above (American Dental Association, 2014). For example, topics of periodontal ligament health and dental implant osteointegration clearly relate to a peculiar "dental orthopedics"; the fact that teeth are not a mineralized appendix but rather a living organ in a living environment (gingiva, oral liquids) involves a specific "dental physiology"; the sensitivity of the living tooth, in connection with the pulp and related nerves, can be associated to a possible "dental neurology." Indeed, the oral environment, which is the place where dental functions are operated, is one of the most complex ones in the human body.As a result, within the dental area several specialized disciplines exist (see Figure 1). Conservative dentistry aims to maintain the original denture and largely overlaps with restorative dentistry, the latter encompassing prosthodontics, periodontics, and endodontics. Prosthodontics (or prosthetic dentistry) is required in case of massive irreversible tooth damage that cannot be fixed with simple resin composite filling, and requires the use of prosthesis for teeth, namely crowns, bridges, and even whole dentures. Periodontics (or periodontology) addresses the diseases of the periodontum, i.e., the teeth ligaments. Endodontics focuses on therapy of the root canals and connected pulp diseases. Oral surgery relates to the extraction of teeth that cannot be saved anymore and should be replaced by implants, and is usually combined with maxillo-facial surgery, extending the treated area around the denture. Prosthodontics, periodontics, and surgery correlate to implantology, which deals with the installation of dental implants. Orthodontics concerns the alignment and straightening of teeth, as well as fixing midface and mandibular growth issues. Pedodontics (or pediatric dentistry) relates to the specific dental issues of children's temporary dentures. Of course, oral...

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Use of Nanotechnology in Dentistry

2022

Nanoparticles for Therapeutic Applications

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Enamel crystals of mice susceptible or resistant to dental fluorosis: an AFM study

Cited by 4 publications

References 24 publications

Morphological features of enamel in fluorosis of different degrees of severity

Morphological features of enamel in fluorosis of different degrees of severity

Dentistry on the Bridge to Nanoscience and Nanotechnology

Use of Nanotechnology in Dentistry

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