The role of Candida albicans in root caries biofilms: an RNA-seq analysis

Ev, Laís Daniela; Damé‐Teixeira, Nailê; Do, Thuy; Maltz, Marisa; Parolo, Clarissa Cavalcanti Fatturi

doi:10.1590/1678-7757-2019-0578

Cited by 10 publications

(7 citation statements)

References 28 publications

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“…More well-designed clinical trials should be conducted to provide information on their clinical uses in older adults. A recent study showed root caries was associated with candidal infection; this may complicate caries management [64]. This implied further investigation in inhibiting the growth of candidal biofilm may be beneficial in caries prevention in the future [65].…”

Section: Discussionmentioning

confidence: 99%

A Systematic Review on Caries Status of Older Adults

Chan

Tamrakar

Jiang

et al. 2021

IJERPH

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The aim of this systematic review was to provide an update on caries prevalence in older adults aged 60 years or above around the globe. Two independent reviewers performed a systematic literature search of English publications from January 2016 to December 2020 using Pubmed, Scopus, Embase/Ovid and Web of Science. The MeSH terms used were “dental caries”, “root caries”, “DMF index”, “aged” and “aged 80 and over”. Further searches in Google Scholar retrieved eight additional publications. The epidemiological surveys reporting the prevalence of dental caries or root caries or caries experience using DMFT (decayed, missing and filled teeth) and DFR (decayed and filled root) in older adults aged 60 years or above were included. Quality of the publications was assessed using the JBI Critical Appraisal Checklist for Studies Reporting Prevalence Data. Among the 5271 identified publications, 39 articles of moderate or good quality were included. Twenty studies were conducted in Asia (China, India, Vietnam, Singapore and Turkey), ten in Europe (Ireland, Norway, Finland, Germany, Portugal, Poland, Romania and Kosovo), three in North America (USA and Mexico), one in South America (Brazil), two in Oceania (Australia) and three in Africa (Malawi, Egypt and South Africa). The prevalence of dental caries ranged from 25% (Australia) to 99% (South Africa), while the prevalence of root caries ranged from 8% (Finland) to 74% (Brazil) in community dwellers. The situation was even worse in institutionalised older adults of which the mean DMFT score varied from 6.9 (Malawi) to 29.7 (South Africa). Based on the included studies published in the last 5 years, caries is still prevalent in older adults worldwide and their prevalence varies across countries.

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Section: Discussionmentioning

confidence: 99%

A Systematic Review on Caries Status of Older Adults

Chan

Tamrakar

Jiang

et al. 2021

IJERPH

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“…Research found that C. albicans genes and proteins related to sugar transportation, pyruvate breakdown and the glyoxylate cycle were upregulated in S. mutans - C. albicans mixed-species biofilms ( Ellepola et al., 2019 ). The transcriptomic analysis of dental plaque from the tooth root surface found that C. albicans genes associated with metabolic activity and glucose transportation were significantly enhanced in the dental caries group compared to the caries-free group ( Ev et al., 2020 ). Our results also demonstrate that S. mutans MVs promote C. albicans carbohydrate metabolism and dentin demineralization provoked by C. albicans biofilms.…”

Section: Discussionmentioning

confidence: 99%

Streptococcus Mutans Membrane Vesicles Enhance Candida albicans Pathogenicity and Carbohydrate Metabolism

Cui

Cao

et al. 2022

Front. Cell. Infect. Microbiol.

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Streptococcus mutans and Candida albicans, as the most common bacterium and fungus in the oral cavity respectively, are considered microbiological risk markers of early childhood caries. S. mutans membrane vesicles (MVs) contain virulence proteins, which play roles in biofilm formation and disease progression. Our previous research found that S. mutans MVs harboring glucosyltransferases augment C. albicans biofilm formation by increasing exopolysaccharide production, but the specific impact of S. mutans MVs on C. albicans virulence and pathogenicity is still unknown. In the present study, we developed C. albicans biofilms on the surface of cover glass, hydroxyapatite discs and bovine dentin specimens. The results showed that C. albicans can better adhere to the tooth surface with the effect of S. mutans MVs. Meanwhile, we employed C. albicans biofilm-bovine dentin model to evaluate the influence of S. mutans MVs on C. albicans biofilm cariogenicity. In the S. mutans MV-treated group, the bovine dentin surface hardness loss was significantly increased and the surface morphology showed more dentin tubule exposure and broken dentin tubules. Subsequently, integrative proteomic and metabolomic approaches were used to identify the differentially expressed proteins and metabolites of C. albicans when cocultured with S. mutans MVs. The combination of proteomics and metabolomics analysis indicated that significantly regulated proteins and metabolites were involved in amino acid and carbohydrate metabolism. In summary, the results of the present study proved that S. mutans MVs increase bovine dentin demineralization provoked by C. albicans biofilms and enhance the protein and metabolite expression of C. albicans related to carbohydrate metabolism.

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“…Furthermore, a significant correlation between S. mutans / S. sanguinis ratio and C. albicans carriage is observed in all recruited subjects ( Du et al, 2021a ). RNA-Seq analysis has demonstrated that C. albicans upregulates the genes associated with C. albicans metabolism, sugar transportation, invasion, stress tolerance, and pH regulation in supragingival plaque of root caries, compared with this fungus in dental plaque of sound root surfaces ( Ev et al, 2020 ). Besides S. mutans , another key root caries pathogen, Actinomyces viscosus , is often found alongside C. albicans in supragingival plaques of root caries patients ( Shen et al, 2002 ).…”

Section: Interactions Between C Albicans and Oral ...mentioning

confidence: 99%

Cross-kingdom interaction between Candida albicans and oral bacteria

Ren

Zhou

et al. 2022

Front. Microbiol.

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Candida albicans is a symbiotic fungus that commonly colonizes on oral mucosal surfaces and mainly affects immuno-compromised individuals. Polymicrobial interactions between C. albicans and oral microbes influence the cellular and biochemical composition of the biofilm, contributing to change clinically relevant outcomes of biofilm-related oral diseases, such as pathogenesis, virulence, and drug-resistance. Notably, the symbiotic relationships between C. albicans and oral bacteria have been well-documented in dental caries, oral mucositis, endodontic and periodontal diseases, implant-related infections, and oral cancer. C. albicans interacts with co-existing oral bacteria through physical attachment, extracellular signals, and metabolic cross-feeding. This review discusses the bacterial–fungal interactions between C. albicans and different oral bacteria, with a particular focus on the underlying mechanism and its relevance to the development and clinical management of oral diseases.

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The role of Candida albicans in root caries biofilms: an RNA-seq analysis

Cited by 10 publications

References 28 publications

A Systematic Review on Caries Status of Older Adults

A Systematic Review on Caries Status of Older Adults

Streptococcus Mutans Membrane Vesicles Enhance Candida albicans Pathogenicity and Carbohydrate Metabolism

Cross-kingdom interaction between Candida albicans and oral bacteria

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