A comparison of the sealing abilities between Biodentine and MTA as root-end filling materials and their effects on bone healing in dogs after periradicular surgery

Abstract

Objectives:To compare the sealing ability and biocompatibility of Biodentine with mineral trioxide aggregate (MTA) when used as root-end filling materials.Methodology:The Cell Counting Kit-8 (CCK-8) assay was used to compare the cytotoxicity of MTA and Biodentine. Twenty-one extracted teeth with a single canal were immersed in an acidic silver nitrate solution after root-end filling. Then, the volume and depth of silver nitrate that infiltrated the apical portion of the teeth were analyzed using micro-computed tomography (micro-CT). Seventy-two roots from 3 female beagle dogs were randomly distributed into 3 groups and apical surgery was performed. After six months, the volume of the bone defect surrounding these roots was analyzed using micro-CT.Results:Based on the results of the CCK-8 assay, MTA and Biodentine did not show statistically significant differences in cytotoxicity (P>0.05). The volume and the depth of the infiltrated nitrate solution were greater in the MTA group than in the Biodentine group (P<0.05). The volume of the bone defect was larger in the MTA group than in the Biodentine group. However, the difference was not significant (P>0.05). The volumes of the bone defects in the MTA and Biodentine groups were smaller than the group without any filling materials (P<0.05).Conclusions:MTA and Biodentine exhibited comparable cellular biocompatibility. Biodentine showed a superior sealing ability to MTA in root-end filling. Both Biodentine and MTA promoted periradicular bone healing in beagle dog periradicular surgery models.

In Vitro Effect of Putty Calcium Silicate Materials on Human Periodontal Ligament Stem Cells

“…Periapical surgery is the last option to save a tooth before performing the extraction when a periapiapical lesion cannot be managed with conventional endodontic retreatment. To achieve the treatment, the material is placed in the root-end cavity sealing the entire area [3][4][5]. This area is where the stem cells from the periodontal ligament (PDLSCs) are located and are in direct contact with the retrograde root filling material [6,7].…”

Cirurgia parendodontica associada a terapia fotodinâmica: relato de caso com acompanhamento de 4 anos

“…Além disso, pesquisas evidenciam que o MTA pode caracterizando-se como um bom material selador apical, mesmo com contato do sangue ou umidade. Sobretudo, as obturações com MTA fornecem ao dente maior resistência à fratura (Tang et al, 2019;Safi et al, 2019).…”

In vitro biocompatibility and bioactivity of calcium silicate‑based bioceramics in endodontics (Review)

“…Furthermore, production of MTA during the hydration reaction and its exposure concentration may result in early slight cytotoxicity of MTA. calcium hydroxide is produced when calcium silicate contained in MTA reacts with water and increases pH of the culture media (55,56).…”

“…Furthermore, production of MTA during the hydration reaction and its exposure concentration may result in early slight cytotoxicity of MTA. Calcium hydroxide is produced when calcium silicate contained in MTA reacts with water and increases pH of the culture media ( 55 , 56 ). Basic pH environment and release of inorganic salts induced by high concentrations of MTA significantly decreases cell proliferation ( 57 ).…”