Effects of triethylene glycol dimethacrylate (TEGDMA) on the odontoclastic differentiation ability of human dental pulp cells

Torun, Zeynep Öncel; Torun, Deniz; Baykal, Baris; Oztuna, Ali; Yeşildal, Fatih; Avcu, Ferit

doi:10.1590/1678-7757-2016-0626

Cited by 7 publications

(6 citation statements)

References 34 publications

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“…This finding confirms previous findings that TC-induced DEGs may be involved in the biological process associated with osteoclastogenesis [26]. Resin monomers have limited potential in promoting human dental pulp cells to differentiate into odontoclasts [50,51]. A previous in vitro study demonstrated that the odontoclastic differentiation ratios were related to resin monomers in a dose-dependent manner [50].…”

Section: Discussionsupporting

confidence: 90%

“…Resin monomers have limited potential in promoting human dental pulp cells to differentiate into odontoclasts [ 50 , 51 ]. A previous in vitro study demonstrated that the odontoclastic differentiation ratios were related to resin monomers in a dose-dependent manner [ 50 ]. Because TC is cured by light, some resin components in the TC may be insufficiently polymerized and consequently released, thus exerting harmful effects on dental pulp cells [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

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Differential Gene Expression Changes in Human Primary Dental Pulp Cells Treated with Biodentine and TheraCal LC Compared to MTA

et al. 2020

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This study aimed to analyze the effects of pulp capping materials on gene expression changes in primary tooth-derived dental pulp cells using next-generation sequencing. Dental pulp cells were extracted and treated with mineral trioxide aggregate (MTA), Biodentine (BD), or TheraCal LC (TC). Cell viability assays were performed. Total RNA was extracted and analyzed through mRNA sequencing. Bioinformatic analysis of differential gene expression in dental pulp cells exposed to BD or TC versus MTA was performed. MTA, BD, and TC exposure had no significant effect on pulp cell viability (p > 0.05). Gene sets associated with inflammatory response (p = 2.94 × 10−5) and tumor necrosis factor alpha (TNF-α) signaling via the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway (p = 2.94 × 10−5) were enriched in all materials. In BD-treated cells, Wnt/β-catenin signaling (p = 3.15 × 10−4) gene sets were enriched, whereas enrichment of interferon gamma (IFN-γ) response (p = 3 × 10−3) was observed in TC-treated cells. In gene plot analysis, marked increases in receptor activator of nuclear factor kappa-Β ligand (RANKL) expression were seen in TC-treated cells over time. Despite the similar cell viabilities exhibited among MTA-, BD-, and TC-treated cells, patterns of gene networks differed, suggesting that diverse functional gene differences may be associated with treatment using these materials.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Differential Gene Expression Changes in Human Primary Dental Pulp Cells Treated with Biodentine and TheraCal LC Compared to MTA

et al. 2020

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“…Inamitsu et al observed that HEMA monomer and TEGDMA inhibit osteoclast differentiation [ 44 ]. Additionally, other researchers have shown that TEGDMA decreases the differentiation capacity of odontoclasts in pulp cells and the differentiation rate of odontoclasts increases proportionally to the amount of TEGDMA monomer [ 45 ]. Yoshii in his study demonstrated that bis-GMA is the most cytotoxic compound, followed by UDMA, TEGDMA and finally MMP [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Study of Cytotoxic Properties of an Experimental Preparation with Features of a Dental Infiltrant

et al. 2021

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Microinvasive dentistry is based on the treatment of early carious lesions with the use of dental infiltrants. The commercially available Icon dental infiltrant does not contain any bacteriostatic component. An experimental preparation enriched with the missing component was synthesised. The aim of this study was to evaluate the cytotoxicity of the experimental preparation. Mouse fibroblasts of the L-929 lineage were used for the in vitro study. Cell morphology and viability were assessed. In the cytotoxicity analysis, it was shown that the experimental preparation (42.8 ± 10.3) after 24 h at two-fold dilution showed similar cytotoxicity to Icon (42.7 ± 8.8) (p > 0.05), while at four-fold dilution experimental preparation (46.7 ± 3.1), it was less toxic than Icon (34.2 ± 3.1) (p < 0.05). The experimental preparation has the potential to provide an alternative to the Icon commercial preparation. Further research is needed to evaluate the cytotoxicity of the experimental preparation over a longer period of time.

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“…Leachable monomers from bonding agents placed directly on dentin or from pulp‐capping agents could lead to pulp inflammation and necrosis (Costa, Hebling, & Hanks, ; Pereira, Segala, & Costa, ). Moreover, monomers, including TEDGMA, inhibit differentiation and mineral deposition of dental pulp cells at a concentration range of 0.1‐3.0 mmol/L (Barelli‐Corbo et al, ; Galler et al, ; Kwon, Park, Zhu, & Yang, ; Oncel Torun et al, ; Spagnuolo et al, ). In contrast, a novel mechanism of dental pulp stem cells, cultured in conditioned media, in TEGDMA‐induced conditions has been reported (Paschalidis et al, ) to increase viability, migration and mineralization potential, which are essential in pulp repair.…”

Section: Introductionmentioning

confidence: 99%

Assessing the effect of triethyleneglycol dimethacrylate on tissue repair in 3D organotypic cultures

Tigani

Škrtić

Valerio

et al. 2018

J of Applied Toxicology

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Leachables from dental restoratives induce toxicity in gingival and pulp tissues and affect tissue regeneration/healing. Appropriate testing of these materials requires a platform that mimics the in vivo environment and allows the architectural self-assembly of cells into tissue constructs. In this study, we employ a new 3D model to assess the impact of triethyleneglycol dimethacrylate (TEGDMA) on early organization and advanced recruitment/accumulation of immortalized mouse gingival fibroblasts (GFs) and dental papilla mesenchymal cells (DPMCs) in extracellular matrix. We hypothesize that TEGDMA (1) interferes with the developmental architecture of GFs and DPMCs, and (2) inhibits the deposition of mineral. To test these hypotheses, GFs and DPMCs were incubated with the soluble TEGDMA at concentrations (0-2.5) mmol/L. Diameter and thickness of the constructs were determined by microscopic analysis. Cell differentiation was assessed by immunocytochemistry and the secreted mineral detected by alizarin-red staining. TEGDMA interfered with the development of GFs and/or DPMCs microtissues in a dose-dependent manner by inhibiting growth of inter-spherical cell layers and decreasing spheroid size (four to six times). At low/moderate TEGDMA levels, GFs organoids retained their structures while reducing thickness up to 21%. In contrast, at low TEGDMA doses, architecture of DPMC organoids was altered and thickness decreased almost twofold. Overall, developmental ability of TEGDMA-exposed GFs and DPMCs depended on TEGDMA level. GFs constructs were more resistant to structural modifications. The employed 3D platform was proven as an efficient tool for quantifying the effects of leachables on tissue repair capacities of gingiva and dental pulp.

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Effects of triethylene glycol dimethacrylate (TEGDMA) on the odontoclastic differentiation ability of human dental pulp cells

Cited by 7 publications

References 34 publications

Differential Gene Expression Changes in Human Primary Dental Pulp Cells Treated with Biodentine and TheraCal LC Compared to MTA

Differential Gene Expression Changes in Human Primary Dental Pulp Cells Treated with Biodentine and TheraCal LC Compared to MTA

Study of Cytotoxic Properties of an Experimental Preparation with Features of a Dental Infiltrant

Assessing the effect of triethyleneglycol dimethacrylate on tissue repair in 3D organotypic cultures

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