Identificação de loci microssatélitescom potencial de amplificação na espécie de Peixe-Rei (Odontesthes humensis)

Tavares, Rafael Aldrighi; Piedras, Sérgio Renato Noguez; Nunes, Mateus Dias; Almeida, Diones Bender; Moreira, Carla Giovane Ávila; Fernandes, João Morato; Freitas, Suzane Fonseca; Moreira, Heden Luiz Marques; Pouey, Juvêncio Luís Osório Fernandes; Dionello, Nelson José Laurino

doi:10.1590/1678-7444

Cited by 1 publication

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References 6 publications

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“…Primer design. The microsatellite primers designed for this study were selected from the results obtained by Tavares et al (2014) who detected, through the genomic library of O. humensis, the "Best PALs" (bPALs), which are SSR loci that present long repeat units (4mers, 5mers, and 6mers) in the populations of O. humensis. In that study, a total of 167 bPALs were generated.…”

Section: Methodsmentioning

confidence: 99%

Diagnostic locus for identification of silverside hybrids (odontesthes humensis and odontesthes bonariensis) in a natural population

Souza¹,

Freitas

Tavares

et al. 2022

Bol. Inst. Pesca

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Hybridization is a natural phenomenon that occurs more often in fish than in other vertebrates. The use of nuclear and mitochondrial molecular markers provides valuable results in the detection of these events. The aim of this study was to investigate the occurrence of interspecific hybrids in natural populations of silverside. The samples of Odontesthes humensis, Odontesthes bonariensis, and individuals that were morphologically different from pure species were collected in the Mangueira lagoon, located in southern Brazil. Result: Six tetranucleotide microsatellite loci were synthesized and tested. The UFPEL_OH3 locus proved to be diagnostic for the detection of silverside hybrids, and it was possible to distinguish between pure and hybrid species. The mitochondrial marker gene cytb synthesized from conserved Odontesthes sequences in the GenBank genetic database showed no differences in the genetic sequence of the samples, needing further studies to confirm the hypothesis.

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Section: Methodsmentioning

confidence: 99%