Comparative study of histopathology and immunohistochemistry of indefinite round cell cutaneous tumors and characterization of canine lymphoma

Abstract

With the purpose of shedding light on some doubts in veterinary oncology, the present article intends to compare the results of histopathological and immunohistochemical examinations of unspecific round cell neoplasia, to realize immunophenotyping of canine lymphoma cases, to establish the T or B origin of neoplastic cells, and to determine the degree of proliferation and apoptosis of lymphomas by immunohistochemistry. Of 11 animals presenting immunohistochemical diagnosis of lymphoma, five had been diagnosed as Lymphoma by HE staining of histopathological slides and six had been classified as unspecific round cell neoplasia. All cases submitted to immunohistochemical examination were T-cell lymphomas. There was a positive correlation between cell proliferation and apoptosis. The comparison among histopathological and immunohistochemical results obtained in the cases examined in the present study suggested that immunohistochemistry is essential for the differentiation of round cell neoplasia. Keywords: dog, histopathology, immunohistochemistry, round cell neoplasia, lymphoma RESUMO Com o objetivo de sanar algumas dúvidas na área da oncologia veterinária, o presente artigo pretende comparar os resultados dos exames histopatológicos e imuno-histoquímicos de neoplasias de células redondas inespecíficas, realizar imunofenotipagem dos casos

Epitheliotropic lymphoma with heart and kidney metastasis in a dog: case report

“…CTCL rarely metastasize (Moore et al, 1994;Yoon et al, 2014); however, when metastasis does occur, is usually restricted to a single organ (Moore et al, 1994). However, Fictum et al (2009), and more recently, Yoon et al (2014) described multisystemic metastasis of CTCL in dogs, both characterized by CD3+ neoplastic cells, as described in our study.…”

“…The most characteristic lesion common to all forms of canine cutaneous T cell lymphoma is the tropism of neoplastic cells in the epithelium (Fontaine et al, 2010). The classic neoplastic cells in canine cutaneous epitheliotropic lymphoma are CD3+ and CD20-cells (Martorell et al, 2011;Machado et al, 2015). More recently, Brachelente et al (2015) observed CD20+ CD3+ expression in CTCL, and this coexpression has been recognized in peripheral T-cell lymphomas.…”

“…The World Health Organization classifies cutaneous T-cell neoplasms as epitheliotropic and nonepitheliotropic. Epitheliotropic neoplasms are normally observed in the classic nodular form (mycosis fungoides) and as pagetoid reticulosis (Martorell et al, 2011). Canine CEL are classified into two types: mycosis fungoides (MF), which is an epitheliotropic form of primary cutaneous T-cell lymphoma (CTCL) and is commonly encountered and Sezary syndrome, which is a rare leukemic variant of CTCL characterized by generalized erythroderma, intense pruritus, lymphadenopathy and leukemia (Rodigheri et al, 2007).…”

“…A study performed by Machado et al (2015) showed that of 11 animals presenting immunohistochemical diagnosis of lymphoma, only five had the diagnosis of lymphoma by HE staining, and six had been classified as unspecific round cell neoplasia, suggesting that immunohistochemistry is essential for the differentiation of round cell neoplasia. In this case, immunohistochemistry was very important to confirm the suspicion of CTCL, given that multiple organ metastasis are very rare.…”

“…Zootec., v.69, n.6, p. [1461][1462][1463][1464][1465]2017 Few studies have shown the occurrence of primary CEL in organs other than the skin (Fontaine et al, 2010). So far only six cases were described in veterinary literature of CTCL with organs metastasis, being four cases in a dog, one in a chipmunk and one in a guinea pig (Czasch et al, 2000;Fictum et al, 2009;Panakova et al, 2009;Martorell et al, 2011;Yoon et al, 2014;Mineshige et al, 2016). This study aims to describe the case of a dog that exhibited a rare manifestation of CEL with multiple organ metastasis, which may have progressed to a paraneoplastic syndrome resulting in mortality.…”