Utilização do filme de quitosana na reparação de tendão em coelhos

Santana, César Costapinto; Neto, P. I. Nóbrega; Sá, Marcelo Jorge Cavalcanti de; Oliveira, Luana Muniz de; Fook, Marcus Vinícius Lia; Azevedo, Adílio Santos de; Sousa, Otávio Brilhante de

doi:10.1590/1678-6521

Cited by 8 publications

(8 citation statements)

References 13 publications

(5 reference statements)

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“…On the 14th day of culture, fixation of the BM-MSCs occurred by interaction with the biomaterial. Consistent with the studies of Alberti & Xu (2016) and Santana, Neto & Sá (2014) , the presence of cytoplasmic projections and normal cell morphology are factors that confirm cytocompatibility between the BCM scaffold and cells.…”

Section: Discussionsupporting

confidence: 83%

Behavior and biocompatibility of rabbit bone marrow mesenchymal stem cells with bacterial cellulose membrane

Silva

Leite

Carvalho

et al. 2018

PeerJ

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BackgroundTissue engineering has been shown to exhibit great potential for the creation of biomaterials capable of developing into functional tissues. Cellular expansion and integration depends on the quality and surface-determinant factors of the scaffold, which are required for successful biological implants. The objective of this research was to characterize and evaluate the in vitro characteristics of rabbit bone marrow mesenchymal stem cells (BM-MSCs) associated with a bacterial cellulose membrane (BCM). We assessed the adhesion, expansion, and integration of the biomaterial as well as its ability to induce macrophage activation. Finally, we evaluated the cytotoxicity and toxicity of the BCM.MethodsSamples of rabbit bone marrow were collected. Mesenchymal stem cells were isolated from medullary aspirates to establish fibroblast colony-forming unit assay. Osteogenic, chondrogenic, and adipogenic differentiation was performed. Integration with the BCM was assessed by scanning electron microscopy at 1, 7, and 14 days. Cytotoxicity was assessed via the production of nitric oxide, and BCM toxicity was assessed with the MTT assay; phagocytic activity was also determined.ResultsThe fibroblastoid colony-forming unit (CFU-F) assay showed cells with a fibroblastoid morphology organized into colonies, and distributed across the culture area surface. In the growth curve, two distinct phases, lag and log phase, were observed at 15 days. Multipotentiality of the cells was evident after induction of osteogenic, chondrogenic, and adipogenic lineages. Regarding the BM-MSCs’ bioelectrical integration with the BCM, BM-MSCs were anchored in the BCM in the first 24 h. On day 7 of culture, the cytoplasm was scattered, and on day 14, the cells were fully integrated with the biomaterial. We also observed significant macrophage activation; analysis of the MTT assay and the concentration of nitric oxide revealed no cytotoxicity of the biomaterial.ConclusionThe BCM allowed the expansion and biointegration of bone marrow progenitor cells with a stable cytotoxic profile, thus presenting itself as a biomaterial with potential for tissue engineering.

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Section: Discussionsupporting

confidence: 83%

Behavior and biocompatibility of rabbit bone marrow mesenchymal stem cells with bacterial cellulose membrane

Silva

Leite

Carvalho

et al. 2018

PeerJ

View full text Add to dashboard Cite

show abstract

“…On the 14th day of culture, fixation of the BM-MSCs occurred by interaction with the biomaterial. Consistent with the studies of Alberti and Xu (2016) and Santana et al (2014), the presence of cytoplasmic projections and normal cell morphology are factors that confirm cytocompatibility between the BCM scaffold and cells. Equilibrium in immune system cell activation also reflects a tissue's regenerative quality.…”

Section: Discussionsupporting

confidence: 83%

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Supplemental Information 14: Effect of the BCM on BM-MSCs and Mammalian Peritoneal Macrophage Viability

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“…Although CG has been evaluated in other studies, research using suture yarns prepared with this material is scarce, thus justifying the experimental use of this yarn in laparorrhaphy and cecorrhaphy. The use of rabbits as experimental models provided important results, because the organism of this animal species reacts against the presence of a foreign body by producing intense cellular reaction, marked by inflammatory reaction of granulomatous characteristic.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, it presumably has a higher tensile strength degradation rate than that expected for synthetic yarns such as poliglecaprone. However, no studies have been carried out with CG yarn on biomechanical tests to quantify the its tensile strength …”

Section: Discussionmentioning

confidence: 99%

Comparison between poliglecaprone and chitosan absorbable sutures in laparorrhaphy and cecorrhaphy in rabbits

et al. 2019

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Despite improvements in the development of sutures and surgical techniques of laparorrhaphy and enterorrhaphy, complications resulting from the process of intestinal and muscular healing after simple enterorrhaphy or enteroanastomoses followed by laparorrhaphy are still frequent. The poliglecaprone 25 (PG) suture is commonly used in this type of surgical procedure due to the induction of little tissue reaction, rapid absorption by hydrolysis, and the absence of capillarity. The chitosan (CG) yarn, despite promising results in in vivo tests, is still rarely used. The objective of this study was to evaluate the use of CG in the procedures of medium laparorrhaphy and cecorrhaphy in rabbits. A total of 42 rabbits were allocated in two groups (n = 21), one referring to raffia procedures using CG and another to postoperatively PG. Macroscopic and microscopic evaluations occurred postoperatively on days 5, 15, and 25. The incidence of postoperative complications such as fibrin accumulation, granulomas, partial dehiscence of the muscular wound, and formation of adhesions was similar in the study groups. In the microscopic evaluation, abdominal muscle healing presented granulation tissue at higher intensity and lower fibrosis at 15 days postoperatively in the CG animals, as well as a greater intensity of polymorphonuclear (PMN) cells in the rabbits' cecorrhaphy area, also at 15 days when compared to PG animals. The conclusion is that the CG yarn comprises biomaterial useful to the laparorrhaphy and cecorrhaphy procedures in rabbits, representing a promising alternative for use in other species and anatomical locations.

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Utilização do filme de quitosana na reparação de tendão em coelhos

Cited by 8 publications

References 13 publications

Behavior and biocompatibility of rabbit bone marrow mesenchymal stem cells with bacterial cellulose membrane

Behavior and biocompatibility of rabbit bone marrow mesenchymal stem cells with bacterial cellulose membrane

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Comparison between poliglecaprone and chitosan absorbable sutures in laparorrhaphy and cecorrhaphy in rabbits

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