Sphingosine 1-phosphate promotes activation of aprine preantral follicle in vitro

Abstract

This study describes the effect of sphingosine 1-phosphate (S1P) for development of preantral follicle, therefore the activation and follicular viability of caprine follicles cultured in vitro. Ovarian fragments were cultured for 1 or 7 days in Minimum Essential Medium with different S1P concentrations (0, 1, 10, 50, 100 or 200ng/mL). All ovarian fragments were processed for histological analysis in optical microscopy, transmission electron microscopy and fluorescence analysis. The treatment using 1ng/mL of S1P was able to maintain the percentage of normal follicles with the progression of the culture from day 1 to 7. At end of the 7-day culture period there was a significant reduction (P<0.05) in the percentage of primordial follicles in all groups treated with S1P, compared with fresh control (FC) and Control Culture (CC), which was followed by an increase of activated follicles (intermediary, primary and secondary). In addition, the culture for 7 days with media supplemented with S1P with 1ng/mL preserved the ultrastructure of organelles and kept the preantral follicular viability when evaluated by fluorescence microscopy. In conclusion, after 7 days of culture, the 1ng/mL of S1P activates the development of preantral caprine follicles, cultured in situ and maintains the oocitary and follicular viability.Keywords: goat, preservation, preantral follicles, sphingosine 1-phosphate, follicular activation S1P (0, 1, 10, 50, 100 ou 200ng/mL RESUMO Este estudo descreve o efeito da esfingosina 1-fosfato (S1P) no desenvolvimento de folículos pré-antrais, portanto da ativação e viabilidade de folículos caprinos cultivados in vitro. Fragmentos de ovários foram cultivados por um ou sete dias em meio essencial mínimo com diferentes concentrações de

Fertility Preservation in Girls and Women: State of Art and Future Possibilities

“…The ability to support oocyte development entirely in vitro would offer an alternative strategy avoiding both the risk of cancer cells reintroduction and the need for the second surgery for transplantation. Moreover, primordial follicles culture would nullify the need for hormonal stimulation after ACOTT [78,79]. The ovarian tissue that is recovered for cryopreservation mainly contains follicles in the early stage of development [80].…”