Herbaspirillum seropedicae as a degrading bacterium of monofluoroacetate: effects of its inoculation in goats by ingesting Amorimia septentrionalis and the concentrations of this compound in plants sprayed with the bacterium

Pessoa, Danielle A.N.; Lopes, José R.G.; Souza, Emanuel Maltempi de; Campos, Édipo Moreira; Medeiros, Rosane M.T.; Cook, Daniel; Lee, Stephen T.; Riet-Correa, Franklin

doi:10.1590/1678-5150-pvb-6305

Cited by 1 publication

(2 citation statements)

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“…In A. septentrionalis samples collected in Pernambuco state, the MFA concentration was practically the same (0.0021%) in the leaves of the adult plant (Albuquerque et al 2014). However, Lopes et al (2019) found 0.00074% MFA in the leaves of A. septentrionalis and Pessoa et al (2019) found 0.16% MFA also in A. septentrionalis leaves; the two plants collected in the same location in Paraíba state. This variation reinforces the claims that plant toxicity varies according to developmental stage (Lee et al 2012, Tokarnia et al 2012, since plants collected in the same region showed variations in toxicity.…”

Section: Introductionmentioning

confidence: 87%

“…Because H. seropedicae is an endophytic bacterium that is capable of colonizing plants, it was cultivated and later used to spray some plants of the species A. septentrionalis, aiming to evaluate whether there was a reduction in the amount of MFA present in them after spraying. Before spraying, the concentration of MFA found in the plants was 1.21±0.53µg/mg, and a significant reduction in the amount of MFA (0.24±0.05µg/mg) was observed eight days after spraying, suggesting the use of endophytic bacteria, which hydrolyze MFA, as a way to reduce the content of this compound in plants (Pessoa et al 2019). Further studies should be conducted to prove these results and demonstrate the permanence of H. seropedicae in A. septentrionalis plants or in other species containing MFA.…”

Section: Oral Administration Of Mfa-degrading Bacteriamentioning

confidence: 98%

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Techniques for prevention and control of poisoning by sodium monofluoroacetate (MFA)-containing plants in ruminants

2019

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Monofluoroacetate (MFA)-containing plants cause sudden death associated with exercise in ruminants, and are responsible for significant losses in Brazilian livestock, estimated at 500,000 bovine deaths annually. Most of the times, the control and treatment of this type of poisoning are not efficient, because disease evolution is superacute, usually causing the death of the animal. Due to the difficulty in controlling this intoxication, several studies have suggested alternatives to prevent it, mainly by making animals resistant to the MFA present in these plants or by avoiding their consumption. This literature review addresses the techniques used experimentally to control the poisoning of ruminants by plants containing MFA. The first studies carried out in Brazil demonstrated that goats and sheep that continuously receive non-toxic doses of plant containing MFA show greater resistance to poisoning than untreated animals, and that this resistance can be transmitted by ruminal fluid transfaunation, suggesting that poisoning occurs due to the presence of bacteria that hydrolyze MFA in the rumen. Based on this hypothesis, several MFA-hydrolyzing bacteria were isolated (Enterococcus faecalis, Bacillus sp., Paenibacillus sp., Burkholderia sp., Cupriavidus sp., Staphylococcus sp., Ancylobacter sp., Ralstonia sp., Stenotrophomonas sp., Pigmentiphaga kullae, and Ancylobacter dichloromethanicus). When some of these bacteria were administered intraruminally, they provided the animal with a different level of protection against poisoning. However, it was observed that protection is gradually lost when the bacterium administration is interrupted. Consequently, to obtain more efficient protection, these bacteria should be administered continuously, probably in the form of probiotics. In another assay, MFA was administered to sheep at non-toxic doses to test the hypothesis that this substance could induce the multiplication of bacteria that hydrolyze it in the rumen. There was no increase in resistance to poisoning after administration of MFA; however, no signs of poisoning were observed when animals received trifluoroacetate and no clinical signs were verified when they were challenged with toxic doses of MFA; in contrast, all control animals presented clinical signs. These results suggest that trifluoroacetate induces the proliferation of MFA-degrading bacteria, and can be used in intoxication prophylaxis. The conditioned food aversion technique, using lithium chloride, has been successfully used experimentally to prevent ruminants from ingesting plants that contain MFA. Another alternative tested was the spraying of Amorimia septentrionalis with the endophytic bacterium Herbaspirillum seropedicae, which degrades MFA, resulting in decreased concentration of this compound in the plants. In conclusion, several experimental techniques have been proved efficient in the control and prophylaxis of MFA-containing plant poisoning; however, none of these techniques are available commercially. Further experiments, mainly in the field, should be carried out to adapt some of these techniques to the conditions of extensive breeding in the numerous areas where MFA-containing plants occur.

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