Experimental reproduction of congenital anomalies in the progeny of cows fed apple pomace during pregnancy

Abstract

This study aimed to describe and discuss the results of an experiment carried out in two stages with pregnant cows fed 25kg/apple pomace/day. The first stage involved 16 pregnant Holstein Friesian cows divided into four groups: Group 0 - Control (5 cows); Group I - 1 month-gestation (4 cows); Group II - 3 month-gestation (4 cows); Group III - 6 month-gestation (3 cows) and was performed from September to December 2015. The second stage comprised 12 pregnant Holstein Friesian cows divided into three groups: Group 0 - Control (6 cows), Group I - 1 month-gestation (3 cows), and Group II - 3 month-gestation (3 cows) and was conducted from April 2016 to February 2017. All study animals received apple pomace at a dose of 25kg/day. As for the first experiment stage, a cow in Group III bred a calf with complete absence of the coccygeal vertebrae and tail, slight bending of the hind limbs, scoliosis in the thoracic spine, and limited mobility. At 30 days, it presented with diarrhea and underdevelopment, and was euthanized for necropsy. At gross examination, malformations were observed in the thoracic spine, coxofemural joint, and genitourinary tract. Regarding the second experiment stage, a cow in Group I gave birth to a calf with curved pelvic and thoracic limbs with thick joints and flattening hooves. Microscopic examination of the femur showed disorganized, irregular hypertrophic zone and scarce growth zone, in addition to primary spongy zone with short, slightly mineralized trabeculae. Samples of the apple pomace used in this study were frozen and sent for laboratory evaluation of pesticide residues, which showed a positive result for the fungicide carbendazim.

Fatores Nutricionais Aplicados à Reprodução de Ruminantes

“…Os fatores nutricionais são expressivos e seus efeitos na superovulação são pouco entendidos. Entre os fatores nutricionais, a vitamina A se destaca e sua importância na reprodução animal é bem conhecida (WICPOLT et al, 2019). A suplementação de vitamina A i.m.…”

“…Dietas contendo elevado teor de proteína, em geral, têm mostrado decréscimo na fertilidade, causando morte embrionária em função do comprometimento do ambiente uterino. O conhecimento dos efeitos da nutrição sobre características reprodutivas no sentido de se estabelecer um adequado manejo nutricional se torna importante para promover uma máxima eficiência reprodutiva dos rebanhos e evitar mudanças em seu perfil hormonal para que não implique diretamente em alterações em sua fisiologia reprodutiva (WICPOLT et al, 2019). Estrada (2000) destacou que uma ração pobre em energia…”