Survey of Salmonella spp. in beef meat for export at slaughterhouses in Brazil

Bier, Daniele; Kich, Jalusa Deon; Duarte, Sabrina Castilho; Silva, Márcio R.; Valsoni, L. M.; Ramos, Carlos A.N.; Rodrigues, Dália P.; Araújo, Flábio R.

doi:10.1590/1678-5150-pvb-5867

Cited by 7 publications

(4 citation statements)

References 38 publications

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“…Consumption of contaminated chicken and pork predisposes humans to Salmonella infection [ 42 , 43 , 96 ]. The presence of Salmonella in cattle in some studies [ 38 , 40 , 55 ] and the possibility of cross-contamination of the carcass in the slaughter of these animals may pose a risk to food safety in the consumption of this type of food [ 97 ].…”

Section: Resultsmentioning

confidence: 99%

Presence of Tetracycline and Sulfonamide Resistance Genes in Salmonella spp.: Literature Review

et al. 2021

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Tetracyclines and sulfonamides are broad-spectrum antibacterial agents which have been used to treat bacterial infections for over half a century. The widespread use of tetracyclines and sulfonamides led to the emergence of resistance in a diverse group of bacteria. This resistance can be studied by searching for resistance genes present in the bacteria responsible for different resistance mechanisms. Salmonella is one of the leading bacteria causing foodborne diseases worldwide, and its resistance to tetracyclines and sulfonamides has been widely reported. The literature review searched the Virtual Health Library for articles with specific data in the studied samples: the resistance genes found, the primers used in PCR, and the thermocycler conditions. The results revealed that Salmonella presented high rates of resistance to tetracycline and sulfonamide, and the most frequent samples used to isolate Salmonella were poultry and pork. The tetracycline resistance genes most frequently detected from Salmonella spp. were tetA followed by tetB. The gene sul1 followed by sul2 were the most frequently sulfonamide resistance genes present in Salmonella. These genes are associated with plasmids, transposons, or both, and are often conjugative, highlighting the transference potential of these genes to other bacteria, environments, animals, and humans.

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Section: Resultsmentioning

confidence: 99%

Presence of Tetracycline and Sulfonamide Resistance Genes in Salmonella spp.: Literature Review

et al. 2021

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“…The Brazilian legislation that supports the surveillance at food-selling points established zero tolerance for S. Enteritidis, S. Typhimurium in meat products (BRASIL, 2019). Studies in Brazil have reported occurrence rates for these pathogens in meat cuts and fresh sausages at retail between 5.80% and 31.50% (BRIZIO et al, 2015;RISTORI et al 2017;BIER et al, 2018;CAVALIN et al, 2018;PERIN et al, 2019). The grinding process used to make ground beef and the ground meat to use in fresh sausages can cause contamination through interactions with remnants from previous processes or dirt, due to hygiene failures (MOLLER et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

Identification of Salmonella spp., Listeria monocytogenes, and indicator microorganisms in commercialized raw meats and fresh sausages from Uruguaiana, Rio Grande do Sul, Brazil

et al. 2021

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This study evaluated the sanitary hygienic quality and the presence of pathogenic microorganisms in raw meats and fresh sausages marketed in the city of Uruguaiana, Rio Grande do Sul (RS), Brazil. We analyzed 238 samples of fresh sausages, beef, pork, and chicken from 18 commercial establishments (butchers, supermarkets, and groceries). Samples were subjected to enumerate hygiene indicator microorganisms (mesophilic aerobes and enterobacteria) and detection of Salmonella spp. and Listeria monocytogenes. The mean counts of mesophilic aerobes and enterobacteria were 5.09 and 3.54 log CFU/g, respectively. Beef samples presented the highest frequency of Salmonella spp. (7.93%) and fresh sausages present the highest frequency of L. monocytogenes (19.04%). Among the analyzed samples, 43.70% did not comply with the microbiological parameters established by the Brazilian Ministry of Health. The presence of Salmonella spp. and L. monocytogenes in different samples and commercial establishments demonstrate the failures of good manufacturing practices in industrial environmental and retails points and the need to train food handlers to reduce the exposure of consumers to potential risks.

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“…In three Brazilian slaughterhouses, 6.7% of the 90 samples collected at three different points in the slaughter line (stunning, washing, and cooling) were positive for Salmonella spp. [ 45 ]. Besides, a study in Mexico isolated Salmonella spp.…”

Section: Discussionmentioning

confidence: 99%

Association between animal welfare indicators and microbiological quality of beef carcasses, including Salmonella spp., from a slaughterhouse in Ecuador

et al. 2021

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Background and Aim: Pre-slaughter management and slaughter operations are considered critical factors for animal welfare and meat quality. Previous studies have found poor animal welfare management at municipal slaughterhouses in Ecuador, and little is known about how this affects the microbiological quality of the meat. Therefore, the aim of the study was to analyze the association of the microbiological quality of beef carcasses and animal welfare indicators in a municipal slaughterhouse in Ecuador. Materials and Methods: Data for 6 months were collected from a municipal slaughterhouse in Ecuador. Five trained researchers were strategically located along the slaughter process. A total of 351 animals were observed with regard to welfare indicators, and their carcasses were sampled to evaluate microbiological quality. Antemortem (slipping, falling, and vocalization) and postmortem animal welfare indicators (bleed interval, pH, temperature, and bruises) were measured. To determine the total aerobic bacteria (TAB) and Escherichia coli counts and the presence of Salmonella spp., we collected samples by swabbing four different points of each carcass. The association between microbiological quality and animal welfare indicators was studied using univariate and multivariate logistic regressions. Results: The mean TAB count was 5.3 log CFU/cm2, and the mean total count of E. coli was 2.4 log CFU/cm2. Salmonella spp. were isolated in 3.1% of the carcasses. An electric goad was used in all animals, 19.1% slipped at least once, and 19.9% vocalized. The mean pH of the carcasses was 7.2, and 79.2% of carcasses had bruises. Multivariate analysis showed that Salmonella spp. and the TAB count were associated with pH and the number of bruises (p = 0.01 in both cases). Conclusion: Although there was non-significant association between the majority of animal welfare indicators and microbiological quality, the poor management affecting animal welfare and carcass hygiene are worrisome.

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Survey of Salmonella spp. in beef meat for export at slaughterhouses in Brazil

Cited by 7 publications

References 38 publications

Presence of Tetracycline and Sulfonamide Resistance Genes in Salmonella spp.: Literature Review

Presence of Tetracycline and Sulfonamide Resistance Genes in Salmonella spp.: Literature Review

Identification of Salmonella spp., Listeria monocytogenes, and indicator microorganisms in commercialized raw meats and fresh sausages from Uruguaiana, Rio Grande do Sul, Brazil

Association between animal welfare indicators and microbiological quality of beef carcasses, including Salmonella spp., from a slaughterhouse in Ecuador

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