Assessment of serum lactate levels, blood glucose values and blood gas values in sheep, newborn lambs and placenta

Silva, Letícia Peternelli; Lourenço, Maria Lúcia Gomes; Paula, Renata Alves de; Verdugo, Mirela Ribeiro; Pereira, Keylla Helena Nobre Pacífico; Chiacchio, Simone Biagio

doi:10.1590/1678-5150-pvb-5689

Cited by 5 publications

(2 citation statements)

References 20 publications

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“…In this trial, the glycaemia values recorded in the lambs within 1 h of parturition agreed with those reported by Vannucchi et al (2012), who considered concentrations of 46.86±21.87 mg/dL as physiological. Furthermore, the glycaemia concentrations recorded in the lambs of the four experimental groups from 24 to 72 h after birth coincided with those reported by Silva et al (2018), who suggested that the elevation of glucose was the result of the contribution of this metabolite through colostrum suckling.…”

Section: Discussionsupporting

confidence: 87%

Subclinical ovine gestational toxaemia at parturition: Influence on determinant variables in lamb survival

Abreu-Palermo,

Neimaur,

Rodríguez-Gamarra

et al. 2023

Revista Brasileira De Zootecnia

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The objective of this study was to determine the impact of metabolic changes occurring in induced subclinical gestation toxaemia in ewes on metabolic parameters and vigour in the first 72 h of their lambs' life. Fifty-one adult Corriedale ewes of known gestation date and fed on a natural pasture were randomly divided into four groups at day 145 of gestation. Group A (ewes with single pregnancies) and group C (ewes with twin pregnancies) were fed on a natural pasture throughout the trial; group B (ewes with single pregnancies) and group D (ewes with twin pregnancies) were subjected to 75% feed restriction for three days until they reached glycaemia and β-hydroxybutyrate (BHB) values indicative of subclinical gestational toxaemia. The birth-to-first station and birth-to-first suckling times of the born lambs were studied. Within the first hour after parturition and at 24, 48, and 72 h, glycaemia and BHB concentrations were determined in the blood of lambs, and body temperature and body weight were assessed at the same time. Relative weight gain at 72 h of age was calculated for all lambs. The BHB concentration determined at all times studied showed no difference among the groups. Subclinical gestation toxaemia induced in ewes subjected to feed restriction causes a decrease in the glycaemia of their lambs in the first hour of life; however, it does not cause an increase in ketone bodies. The metabolic changes induced in the mothers do not have negative effects on the vigour of the lambs at birth, as the weight, temperature, and the time it took for lambs to stand and suckle are not affected. However, they have a negative effect on relative weight gain in the critical period of the first 72 h of life.

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Section: Discussionsupporting

confidence: 87%

Subclinical ovine gestational toxaemia at parturition: Influence on determinant variables in lamb survival

Abreu-Palermo,

Neimaur,

Rodríguez-Gamarra

et al. 2023

Revista Brasileira De Zootecnia

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“…Monitoring the inflammatory response is a challenge because the signs of inflammation are not always clear [27]. Hemogasometry is an important technique for assessing acid-base balance in various diseases, capable of clarifying severity and predicting clinical evolution [15,26,28]. However, in the acute phase of disease, it may not detect changes suggestive of inflammatory process [1].…”

Section: Introductionmentioning

confidence: 99%

Predictive Elements of Obstructive Urolithiasis in Sheep*

Maciel

Oliveira

Afonso

et al. 2019

Acta Scientiae. Vet.

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Background: Urolithiasis is an economically important disease that has considerable significance for sheep farming. With the tissue and biochemical changes resulting from the development of this disease, metabolic disorders and immune response are established. Hemogasometric evaluation allows the identification of systemic acid-base imbalances quickly. Acute phase proteins (AFP) have in the last two decades become the biomarkers of choice in human and veterinary medicine. To date, no biomarker studies have been published for sheep with obstructive urolithiasis. Thus, this study aimed to analyze the hemogasometric kinetics in obstructive urolithiasis in sheep and the AFP that can be used as early biomarkers in this disease.Materials, Methods & Results: In this study, 14 healthy male Santa Inês sheep, aged approximately 90 days, fed on calculogenic diet for 120 days. The animals were examined weekly, including observation of clinical signs, and blood and urine analysis were performed. For comparative analysis purposes, at the end of the experiment, sheep that developed obstructive urolithiasis were extracted from the initial experimental group D1 (without urolithiasis) and became part of the second experimental group D2 (with urolithiasis). In the pre-experimental period and on the day of slaughter, venous blood samples were harvested for hemogasometric tests, with a maximum time of 15 minutes between collection and analysis, to ensure the reliability of the results obtained. The pH, pCO2, pO2, EB, tCO2, HCO3-, stHCO3-, tHb, sO2 and Hct, Na+, K+ and Ca2+ ions were quantified. To identify and measure immunoglobulins (A and G) and AFP, samples from sheep that developed obstructive urolithiasis (D2) were analyzed. Blood samples were harvested weekly until the clinical manifestation of the disease, totaling 16 samples, when IgA, IgG, ceruloplasmin, transferrin, albumin, α1-antitrypsin, haptoglobin and α1-acid glycoprotein concentrations were measured. Elevation of pCO2 was observed between D1 and D2, but there was a significant difference (P < 0.05) only in the final moments (FMs). Although EB, tCO2, HCO3-, stHCO3- increased between moments in the same group and between groups at the same time, significant differences were recorded only in the FMs. Higher values were observed for Na+, K+ in the FMs. The AFP of sheep that developed the disease oscillated between moments, however, significant difference (P < 0.05) over time was observed only in haptoglobin and transferrin.Discussion: The disease occurred in five of the 14 animals studied, demonstrating the effectiveness of the formulated diet in inducing the disease. Through the analysis of blood gases, plasma bicarbonate concentration and excess base or deficit it was possible to diagnose disturbances in acid base balance, characterizing a picture of metabolic alkalosis in animals with urolithiasis. Mean pH was not significantly different between groups, but sheep that developed urolithiasis had alkalosis. Final values of tCO2 and HCO3- indicate the compensatory organic response, which, together with the analysis of the averages of HCO3- and EB, reflect the metabolic alkalosis picture. The AFP have different responsiveness among them. Haptoglobin and transferrin were the most reliable biomarkers among the studied AFP to predict obstructive urolithiasis, with transferrin showing atypical behavior, characteristic of positive AFP.

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Impact of different dexamethasone treatment protocols in goats for preterm neonatal outcomes

Narciso

Alcindo

Bovino³

et al. 2022

Small Ruminant Research

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Assessment of serum lactate levels, blood glucose values and blood gas values in sheep, newborn lambs and placenta

Cited by 5 publications

References 20 publications

Subclinical ovine gestational toxaemia at parturition: Influence on determinant variables in lamb survival

Subclinical ovine gestational toxaemia at parturition: Influence on determinant variables in lamb survival

Predictive Elements of Obstructive Urolithiasis in Sheep*

Impact of different dexamethasone treatment protocols in goats for preterm neonatal outcomes

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