Draft genome sequence of Wickerhamomyces anomalus LBCM1105, isolated from cachaça fermentation

Cunha, Aureliano Claret da; Santos, Renato Augusto Corrêa dos; Riaño-Pachón, Diego Mauricio; Squina, Fábio M.; Oliveira, Juliana Velasco Castro de; Goldman, Gustavo H.; Souza, Aline Tieppo de; Gomes, Lorena Soares; Godoy-Santos, Fernanda; Teixeira, J. A.; Faria-Oliveira, Fábio; Rosse, Izinara Cruz; Castro, Ieso Miranda; Lucas, Cândida; Brandão, Rogélio Lopes

doi:10.1590/1678-4685-gmb-2019-0122

Cited by 7 publications

(4 citation statements)

References 25 publications

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“…This temperature range is narrower than that exhibited by isolates of the yeast-like fungus Aureobasidium subglaciale , which grew from 0°C to 30°C, and even tolerated incubation at 50°C for 2 h ( Zajc et al, 2022 ). The sizes of draft genomes and GC contents ranged from 20.7 to 33.8 Mpb and 50.9% to 61.2%, respectively, in agreement with other yeasts isolated from cold habitats ( Brejová et al, 2017 ; Coleine et al, 2019 , 2020 ; Cunha et al, 2020 ; Baeza et al, 2021 ). Comparing the calculated protein flexibilities among yeasts, significant differences were found mainly for medium plus very flexible (M1 + 2, predicted by MEDUSA), which was higher for G. martinii than G. gilvescens and N. antarctica , and for P. glacialis than G. gilvescens and N. antarctica .…”

Section: Discussionsupporting

confidence: 83%

A genomic approach to analyze the cold adaptation of yeasts isolated from Italian Alps

Turchetti

Buzzini²,

Baeza³

2022

Front. Microbiol.

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Microorganisms including yeasts are responsible for mineralization of organic matter in cold regions, and their characterization is critical to elucidate the ecology of such environments on Earth. Strategies developed by yeasts to survive in cold environments have been increasingly studied in the last years and applied to different biotechnological applications, but their knowledge is still limited. Microbial adaptations to cold include the synthesis of cryoprotective compounds, as well as the presence of a high number of genes encoding the synthesis of proteins/enzymes characterized by a reduced proline content and highly flexible and large catalytic active sites. This study is a comparative genomic study on the adaptations of yeasts isolated from the Italian Alps, considering their growth kinetics. The optimal temperature for growth (OTG), growth rate (Gr), and draft genome sizes considerably varied (OTG, 10°C–20°C; Gr, 0.071–0.0726; genomes, 20.7–21.5 Mpb; %GC, 50.9–61.5). A direct relationship was observed between calculated protein flexibilities and OTG, but not for Gr. Putative genes encoding for cold stress response were found, as well as high numbers of genes encoding for general, oxidative, and osmotic stresses. The cold response genes found in the studied yeasts play roles in cell membrane adaptation, compatible solute accumulation, RNA structure changes, and protein folding, i.e., dihydrolipoamide dehydrogenase, glycogen synthase, omega-6 fatty acid, stearoyl-CoA desaturase, ATP-dependent RNA helicase, and elongation of very-long-chain fatty acids. A redundancy for several putative genes was found, higher for P-loop containing nucleoside triphosphate hydrolase, alpha/beta hydrolase, armadillo repeat-containing proteins, and the major facilitator superfamily protein. Hundreds of thousands of small open reading frames (SmORFs) were found in all studied yeasts, especially in Phenoliferia glacialis. Gene clusters encoding for the synthesis of secondary metabolites such as terpene, non-ribosomal peptide, and type III polyketide were predicted in four, three, and two studied yeasts, respectively.

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Section: Discussionsupporting

confidence: 83%

A genomic approach to analyze the cold adaptation of yeasts isolated from Italian Alps

Turchetti

Buzzini²,

Baeza³

2022

Front. Microbiol.

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“…(size 19 Mb, GC content 60%) ( Coleine et al, 2020 ), and Exophiala mesophila (size 30 Mb, GC content 50%) ( Coleine et al, 2019 ). Although the percentages of predicted CDSs that were annotated ranged from 10 to 28%, the worst results were obtained for W. anomalus (genome size of 19.8 Mb, and 23,034 predicted CDSs), in which only 2,488 CDSs were annotated, which was smaller than that of W. anomalus isolated from fermentations (6,766 to 7,444 annotated CDSs) ( Schneider et al, 2012 ; Cunha et al, 2020 ), but furthermore, the GC content differed by approximately 1.5-fold. In all genomes, putative genes responsible for secondary metabolite production and gene clusters for type III PKS, NRPS and terpenes were found, with the last two being commonly observed in fungi.…”

Section: Discussionmentioning

confidence: 93%

Identification of Stress-Related Genes and a Comparative Analysis of the Amino Acid Compositions of Translated Coding Sequences Based on Draft Genome Sequences of Antarctic Yeasts

et al. 2021

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Microorganisms inhabiting cold environments have evolved strategies to tolerate and thrive in those extreme conditions, mainly the low temperature that slow down reaction rates. Among described molecular and metabolic adaptations to enable functioning in the cold, there is the synthesis of cold-active proteins/enzymes. In bacterial cold-active proteins, reduced proline content and highly flexible and larger catalytic active sites than mesophylls counterparts have been described. However, beyond the low temperature, microorganisms’ physiological requirements may differ according to their growth velocities, influencing their global protein compositions. This hypothesis was tested in this work using eight cold-adapted yeasts isolated from Antarctica, for which their growth parameters were measured and their draft genomes determined and bioinformatically analyzed. The optimal temperature for yeasts’ growth ranged from 10 to 22°C, and yeasts having similar or same optimal temperature for growth displayed significative different growth rates. The sizes of the draft genomes ranged from 10.7 (Tetracladium sp.) to 30.7 Mb (Leucosporidium creatinivorum), and the GC contents from 37 (Candida sake) to 60% (L. creatinivorum). Putative genes related to various kinds of stress were identified and were especially numerous for oxidative and cold stress responses. The putative proteins were classified according to predicted cellular function and subcellular localization. The amino acid composition was compared among yeasts considering their optimal temperature for growth and growth rates. In several groups of predicted proteins, correlations were observed between their contents of flexible amino acids and both the yeasts’ optimal temperatures for growth and their growth rates. In general, the contents of flexible amino acids were higher in yeasts growing more rapidly as their optimal temperature for growth was lower. The contents of flexible amino acids became lower among yeasts with higher optimal temperatures for growth as their growth rates increased.

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“…The biotechnological potential of this yeast and its use in different industrial applications derive from capability of W. anomalus to secrete antimicrobials factors acting on a broad spectrum of pathogens ( Walker, 2011 ). In the last decade, the yeast has drawn special attention and the genome sequences of two environmental strains have been published ( Schneider et al, 2012 ; Cunha et al, 2020 ). The European Food Safety Authority (EFSA) classifies W. anomalus at biosafety level one and different “killer strains” (yeast isolates that produce KTs) are used as biocontrol agents with inhibitory effects against mold and bacteria in the agro-food sector ( Sundh and Melin, 2011 ).…”

Section: General Features Of Wickerhamomyces Anomalusmentioning

confidence: 99%

Wickerhamomyces anomalus in Mosquitoes: A Promising Yeast-Based Tool for the “Symbiotic Control” of Mosquito-Borne Diseases

2021

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The ascomycete yeast Wickerhamomyces anomalus is a mutualistic symbiont of different insects, including diptera vectors of diseases. Although fungal symbioses have been so far poorly characterized, the topic is gaining attention as yeast-insect interactions can provide pivotal information on insect biology, such as their environmental adaptation or vectorial capability. We review the symbiosis between W. anomalus and mosquitoes, which implies nutritional and protective functions. Furthermore, we focus on antiplasmodial effects of W. anomalus in malaria vectors and discuss the yeast potential for the “symbiotic control” (SC) of mosquito-borne diseases (MBDs).

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Draft genome sequence of Wickerhamomyces anomalus LBCM1105, isolated from cachaça fermentation

Cited by 7 publications

References 25 publications

A genomic approach to analyze the cold adaptation of yeasts isolated from Italian Alps

A genomic approach to analyze the cold adaptation of yeasts isolated from Italian Alps

Identification of Stress-Related Genes and a Comparative Analysis of the Amino Acid Compositions of Translated Coding Sequences Based on Draft Genome Sequences of Antarctic Yeasts

Wickerhamomyces anomalus in Mosquitoes: A Promising Yeast-Based Tool for the “Symbiotic Control” of Mosquito-Borne Diseases

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