Spatiotemporal expression of MYD88 gene in pigs from birth to adulthood

Abstract

MYD88 plays an important role in the immune response against infections. To analyze MYD88 gene expression during different stages of pig development, we used real-time PCR. MYD88 was seen expressed in all tissues examined. MYD88 expression in spleen, lungs, and thymus reached its highest value from 7 to 14 days of age and decreased thereafter. Expression in lymph nodes was high until 28 days of age and then it declined after weaning, with stable low levels in adult pigs. MYD88 expression was high before 35 days of age in the small intestine (duodenum, jejunum, and ileum), where it reached its highest value from 7 to 14 days of age. MYD88 expression in the small intestine declined post-weaning and remained relatively low during adulthood. The results of this study suggest that weaning stress and development of the immune system might be positively correlated with MYD88 expression regulation. Moreover, this study provided evidence that the high expression of MYD88 may diminish weaning stress and increase disease resistance in Meishan pigs.

Developmental Changes of Immunity and Different Responses to Weaning Stress of Chinese Indigenous Piglets and Duroc Piglets during Suckling and Weaning Periods

“…The present study showed that ileal IRAK1, CD14, MyD88, NF-κB, and RIP2 expression levels of DR piglets were up-regulated at 24 days of age in comparison with the TB and XB piglets. These results are in agreement with previous studies that reported that weaning stress up-regulates the expression levels of MyD88 and NF-κB, which are associated with immunity and inflammation of piglets during weaning, and these expressions declined after weaning [40,41], which also coincides with the changes in immune cytokines levels in the present study. These findings suggest that the weaning stress in Chinese indigenous pigs is stronger than that in DR pigs, and this different mechanism may be related to the inhibition of the MyD88/NF-κB signaling pathway.…”

Cardiac MyD88 Mediates Inflammatory Injury and Adverse Remodeling in Diabetes

“…In addition, MyD88 is expressed in the heart, liver, spleen, lungs, kidney, thymus, lymph nodes and digestive systems at different developmental stages, indicating its important role. [34] However, the systemic MyD88 knockout mice were viable without any overt phenotype, [7] indicating that MyD88 is dispensable physiologically and strategies to inhibit MyD88 at any stage of life are expected not to show strong side-effects in other cell-type or organ systems. This study validates this deduction by good e ciency and high safety of LM8 in both type 1 and type 2 diabetic mice.…”