2017
DOI: 10.1590/1678-4685-gmb-2016-0107
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Abstract: Urease catalyzes the hydrolysis of urea to ammonia and carbon dioxide. The ammonia (nitrogen (N) product of urease activity) is incorporated into organic compounds. Thus, urease is involved in N remobilization, as well as in primary N assimilation. Two urease isoforms have been described for soybean: the embryo-specific, encoded by the Eu1 gene, and the ubiquitous urease, encoded by Eu4. A third urease-encoding gene was recently identified, designated Eu5, which encodes the putative protein product SBU-III. Th… Show more

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Cited by 15 publications
(9 citation statements)
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References 46 publications
(74 reference statements)
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“…An important finding from this taxonomic survey was that BH was not detected in land plants or animals. In contrast, urease is active in some invertebrates and nearly all plants (McDonald and Campbell, ; Hanlon, ; Rechenmacher et al ., ). In Arabidopsis , soybeans, and other crop plants, urease is essential for plant development, nitrogen remobilization and primary nitrogen assimilation (Cao et al ., ; Rechenmacher et al ., ).…”
Section: Resultsmentioning
confidence: 97%
See 1 more Smart Citation
“…An important finding from this taxonomic survey was that BH was not detected in land plants or animals. In contrast, urease is active in some invertebrates and nearly all plants (McDonald and Campbell, ; Hanlon, ; Rechenmacher et al ., ). In Arabidopsis , soybeans, and other crop plants, urease is essential for plant development, nitrogen remobilization and primary nitrogen assimilation (Cao et al ., ; Rechenmacher et al ., ).…”
Section: Resultsmentioning
confidence: 97%
“…In contrast, urease is active in some invertebrates and nearly all plants (McDonald and Campbell, ; Hanlon, ; Rechenmacher et al ., ). In Arabidopsis , soybeans, and other crop plants, urease is essential for plant development, nitrogen remobilization and primary nitrogen assimilation (Cao et al ., ; Rechenmacher et al ., ). We speculate biuret is an inaccessible nitrogen source to plants and represents a nutrient pool that can only be tapped by BH‐mediated metabolism by soil‐ and plant‐associated microbes.…”
Section: Resultsmentioning
confidence: 97%
“…Concanavalin and soybean contain urease [13], which catalyzes the hydrolysis process of urea. Rechenmacher et al [14] has reported that there are three urease isoforms in soybean: the embryospecific encoded by the Eu1 gene, as well as the ubiquitous urease encoded by Eu4 and Eu5.Pure urease extracted from legumes can produce a kit for clinical diagnosis of urea ammonia in serum, regeneration of the dialysate solution in the wearable kidney machine [15].When urease and urea are present at the same time, it provides N nutrition of high concentration, which is continually beneficial to seed germination, plant development [14] and bioregenerative life support systems [16]. Moreover, ureases play a role in defense against insect and phytopathogenic fungi [17] .It was reported that it can increase the hydrolysis rate of urea up to 10 14 times [15].…”
Section: Introductionmentioning
confidence: 99%
“…Urease, as the earliest discovered nickel metalloenzyme, has attracted extensive research interest for a long time since being separated from the crystalline protein. , Urease plays a critical role in various metabolisms of organisms, which can catalyze the decomposition of urea into ammonia and CO 2 . Many urinary and gastrointestinal diseases including cancer and pyelonephritis are closely related to urease activity, and the sensing of urease activity has been used to diagnose some diseases . Noteworthily, the urease activity in the oral cavity is recognized as an important diagnostic biomarker for early disease diagnosis .…”
Section: Introductionmentioning
confidence: 99%
“…3 Many urinary and gastrointestinal diseases including cancer and pyelonephritis are closely related to urease activity, 4 and the sensing of urease activity has been used to diagnose some diseases. 5 Noteworthily, the urease activity in the oral cavity is recognized as an important diagnostic biomarker for early disease diagnosis. 6 Up to date, various methods such as fluorescence, 7 ELISA, 8 electrochemistry, 9 and colorimetric 10,11 were applied for the analysis of urease.…”
Section: ■ Introductionmentioning
confidence: 99%