Enrichment of nutritional value of Phyllanthus emblica fruit juice using the probiotic bacterium, Lactobacillus paracasei HII01 mediated fermentation

Kesika

et al. 2017

Asian J Pharm Clin Res

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Objective: Plants are the vital source of pharmaceutically important compounds with less or no adverse side effects. The current study was conducted to catalog the commonly used indigenous and medicinal plants of Thailand based on their phenolic acid content and antioxidant activity. Methods:The herbs were collected from Chiang Mai province, Thailand. The plants were extracted with 70% ethanol. The total phenolic acid content and antioxidant activity were evaluated. Results:The ethanolic extract of plant samples was prepared. Among the tested plant samples, Phyllanthus emblica Linn. and Terminalia belerica Roxb. showed highest phenolic content (Gallic acid equivalent [GAE]; 764.81 mg GAE/g sample) and antioxidant activity (trolox equivalent antioxidant capacity [TEAC]; 394.20 mg/g sample), respectively. About 94-97% of inhibition of free radical was detected in 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay tested against the plant samples. The ethanolic extract of Anaxagorea luzonensis Gray., Terminalia sp., T. belerica Roxb, Terminalia chebula Retz., Albizia procera Benth., Harrisonia perforata Merr., and P. emblica Linn. exhibited 97.87, 96.08, 92.26, 86.74, 86.08, 84.47, and 83.13% of superoxide radical inhibition, respectively. Conclusion:The results suggested that T. belerica Roxb. possessed high TEAC ability and DPPH radical scavenging capacity and A. luzonensis Gray. exhibited high superoxide scavenging activity, when compared to that of the other tested samples. The additional detailed study is desirable to understand the complexity and distribution of bioactive compounds present in the commonly used plant species of Thailand.

Section: Resultsmentioning

confidence: 99%

Total Phenolic Content and Free Radical Scavenging Activity of Representative Medicinal Plants of Thailand

Kesika

et al. 2017

Asian J Pharm Clin Res

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“…Total antioxidant capacity (TAC) [13], reducing power [14], and hydrogen peroxide scavenging activity [15] of M. tinctoria aqueous extract were assessed as prescribed earlier. The free radical scavenging assays such as 1, 1-diphenyl 2-picrylhyorazyl (DPPH) and 2, 2-Azinobis-(3 ethylbenzothiazoline-6-sulfonic acids) (ABTS) were performed to determine the free radical scavenging property of M. tinctoria extract as detailed in the previous studies [15][16][17].…”

Section: Assessment Of Antioxidant Activitiesmentioning

confidence: 99%

“…The free radical scavenging assays such as 1, 1-diphenyl 2-picrylhyorazyl (DPPH) and 2, 2-Azinobis-(3 ethylbenzothiazoline-6-sulfonic acids) (ABTS) were performed to determine the free radical scavenging property of M. tinctoria extract as detailed in the previous studies [15][16][17].…”

Section: Assessment Of Antioxidant Activitiesmentioning

confidence: 99%

Evaluation of Bioactivities of Morinda Tinctoria Leaves Extract for Pharmacological Applications.

Sivakumar¹,

Sivamaruthi

Priya³

et al. 2018

Asian J Pharm Clin Res

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Objective: The present study was aimed to prepare Morinda tinctoria leaves extracts with the different solvent system and to evaluate the bioactivities. Methods:The extracts of M. tinctoria were qualitatively analyzed for the primary phytochemical content. The functional groups of extract were determined by Fourier-transform infrared spectroscopy (FT-IR) analysis. The antimicrobial properties were determined by plate assays. The antioxidant and in vitro antiinflammatory properties and membrane stabilizing nature of aqueous extract of M. tinctoria (AEM) were measured using a spectrophotometer. Results:The aqueous, ethanolic, and acetone extracts of M. tinctoria were prepared. AEM contains quinones, steroids, terpenoids, phenols, glycosides, and tannins. FTIR result showed that AEM comprises of alkyl halides, 1°, 2° amines, aromatics, aliphatic amines, alcohols, carboxylic acids, esters, ethers, and alkanes, saturated aliphatic, and phenolic groups. The antimicrobial property of M. tinctoria varied based on the solvent used for the extraction. About 86.90±0.36, 78.58±0.13, and 80.33±0.09% of total antioxidant capacity, reducing power, and hydrogen peroxide scavenging activity were observed in AEM, respectively. The 1, 1-diphenyl 2-picrylhyorazyl and 2, 2-Azinobis-(3 ethylbenzothiazoline-6-sulfonic acids) assay results indicated 85.20±0.50 and 52.41±0.60% of free radical scavenging activity in AEM. The protease activity (44.10±0.26%) and protein degradation (44.38±0.58%) were proscribed by AEM. AEM prevents 69.36±0.20% of cell lysis. Conclusion:The results revealed that the AEM leaves were harmless and enriched with potent bioactive principles, which is further used for food and pharmacological applications.

Natl J Physiol Pharm Pharmacol

“…The quantity of the lactic acid during the fermentation process was measured by (High-Performance Liquid Chromatography; Advanced Chromatography Technologies, Scotland), [21] and the pH value of the sample was determined by pH meter (Metrohm 691). [21] …”

Section: Quantification Of Lactic Acid and Ph In Fsmmentioning

confidence: 99%

Screening of lactic acid bacteria with immune modulating property, and the production of lactic acid bacteria mediated fermented soymilk

Sirilun¹,

Chaiyasut²,

Kesika³

et al. 2017

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Background: Lactic acid bacteria (LAB) are reported to have immune activating property. The genetically modified LAB strains producing interleukin (IL) -10 were under consideration for the treatment of inflammatory bowel diseases (IBD). Soybean is rich in necessary nutrients, and the fermented soybean products are known for its health benefits. Aims and Objectives: The current study was aimed to screen LAB with the immune activating property, and production and evaluation of LAB mediated fermented soymilk product. Materials and Methods: Screening of LAB from the fermented foods collected in Chiang Mai. To select a potent LAB strain, the LAB isolates were scrutinized for acid tolerant, bile tolerant, growth rate, antimicrobial ability, adhesion property, and induction of IL-10 production. The physical properties of fermented soymilk (FSM) were evaluated, and in vitro activation of IL-10 by FSM was assessed using Caco-2 cell line. Results: About 742 LAB strains were isolated from 335 fermented food samples. Based on the tested properties, LAB33 was selected as a potent LAB strain, and used for the fermentation of soymilk for 30 days. The LAB33 FSM was superior in quality regarding probiotic nature and microbiological safety. Conclusion: The results of the study suggested that LAB33 is the potent probiotic strain for the production of soymilk-based fermented functional food supplements for the betterment of IBD patients. Further, strain identification is required to register the strain in a public repository, and sustainable utilization for the benefit of mankind.