Enhance of Cellulase Production and Biomass Degradation by Transformation of the Trichoderma reesei RUT-C30∆zface1 Strain

Abstract

The second-generation bioethanol employs lignocellulosic materials degraded by microbial cellulases in their production. The fungus Trichoderma reesei is one of the main microorganisms producing cellulases, and its genetic modification can lead to the optimization in obtaining hydrolytic enzymes. This work carried out the deletion of the sequence that encodes the zinc finger motif of the transcription factor ACE1 (cellulase expression repressor I) of the fungus T. reesei RUT-C30. The transformation of the RUT-C30 lineage was confirmed by amplification of the 989 bp fragment relative to the selection marker, and by the absence of the zinc finger region amplification in mutants, named T. reesei RUT-C30Δzface1. The production of cellulases by mutants was compared to RUT-C30 and measured with substrates carboxymethylcellulose (CMC), microcrystalline cellulose (Avicel ® ) and Whatman filter paper (PF). The results demonstrated that RUT-C30Δzface1 has cellulolytic activity increased 3.2-fold in Avicel and 2.1-fold in CMC and PF. The mutants presented 1.4-fold higher sugar released in the hydrolysis of the biomass HIGHLIGHTS  Enhance of Trichoderma reesei RUT-C30∆zface1. Deletion of zinc finger of the repressor transcription factor cellulase ACE1. Optimized fungal strain for the production of cellulase. Greater efficiency in the enzymatic activity and sugarcane hydrolysis. 2 Dudek, D.N.; et al.

Determination of Bioethanol Production from Apricot (Prunus armeniaca) Pomace

“…These researches mainly focus on sustainable, renewable and cheap sources such as biofuels. Among these biofuels, bioethanol is considered one of the most promising ones [1,2]. Because bioethanol production from renewable sources can reduce CO2 emissions up to 90% and reduce the dependence on fossil fuels, its usage in certain areas is a reasonable option for sustainable HIGHLIGHTS  Bioethanol production from apricot pomace was reported for the first time.…”