PI3K/Akt Signaling Involved with Osteoinductive Effects of Achatina fulica Mucus

Kantawong, Fahsai; Jearasakwattana, Thananat; Nira, Alisa; Chewae, Jumaila; Sajjamongkol, Phusanisa; Phothong, Pajaree; E-kobon, Teerasak; Chumnanpuen, Pramote

doi:10.1590/1678-4324-2020180501

Cited by 1 publication

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“…fulica mucus, which recently started gaining the attention of researchers for its potential anticancer properties, wound healing, and antibacterial properties. ,− The osteoinductive properties of A. fulica mucus have been reported . In their study, Kantawong and his group demonstrated that A.…”

Section: Introductionmentioning

confidence: 99%

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Snail Mucus from Achatina fulica as a Biomaterial Exhibits Pro-Survival Effects on Human Chondrocytes

Ajisafe,

Raichur

2023

ACS Biomater. Sci. Eng.

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This study reports the novel use of Achatina fulica (A. fulica) mucus as a potential therapeutic repair agent in osteoarthritis and cartilage tissue repair in vitro. Snail mucus was isolated, sterilized, and characterized using FTIR, XPS, rheology, and LC−MS/MS. The GAGs, sugar, phenol, and protein contents were estimated using standard assays. The LC−MS/MS identified 6-gingerol and some other small molecules. The effects of the sterilized mucus were studied on human chondrocytes using the C28/I2 cell as a model for the in vitro assays. The MTT assay indicates that mucus extracted from the pedal of A. fulica is biocompatible with the cells up to a concentration of 50 μg/mL. The mucus promoted cell migration and proliferation and completely closed the wound within 72 h, as indicated in the in vitro scratch assay. In addition, the snail mucus reduced apoptosis significantly (p < 0.05) in the treated cells by 74.6%. It preserved the cytoskeletal integrity of the C28/I2 cells, attributed mainly to GAGs and 6-gingerol content of the mucus. In conclusion, this present study suggests that GAGs and 6-gingerol conferred wound-healing and antiapoptotic properties on the mucus secretion from A. fulica and can be explored for therapeutic repair and cartilage tissue engineering.

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Section: Introductionmentioning

confidence: 99%

“…fulica mucus increased the expression of PI3K. When supplemented with polycaprolactone, the effect impacted human mesenchymal stem cells (hMSC) positively and increased the expressions of Runt-related transcription factor (RUNX) and osteopontin (OPN) . Meanwhile, Kantawong et al reported that A.…”

Section: Introductionmentioning

confidence: 99%

Snail Mucus from Achatina fulica as a Biomaterial Exhibits Pro-Survival Effects on Human Chondrocytes

Ajisafe,

Raichur

2023

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

show abstract

PI3K/Akt Signaling Involved with Osteoinductive Effects of Achatina fulica Mucus

Cited by 1 publication

References 28 publications

Snail Mucus from Achatina fulica as a Biomaterial Exhibits Pro-Survival Effects on Human Chondrocytes

Snail Mucus from Achatina fulica as a Biomaterial Exhibits Pro-Survival Effects on Human Chondrocytes

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