In vitro Antiproliferative and inhibition of oxidative DNA damage activities of n-butanol extract of Limonium bonduelli from Algeria

Abstract

Plants are the main sources of natural antioxidants in the form of phenolic compounds, which help human beings to deal with oxidative stress, caused by free radical damage. For this reason, the present study was carried out to evaluate the antiproliferative, antioxidant and inhibition of oxidative DNA damage activities of n-butanol extract obtained from aerial parts of Limonium bonduelli. The antioxidant potential was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and inhibition of lipid peroxidation assay. Antiproliferative activity was evaluated using xCELLigence RTCA instrument on two tumor cell lines; HT-29 (human colon adenocarcinoma) and HeLa (human cervix carcinoma). DNA damage inhibition was evaluated using photolyzing 46966 plasmid. Also, total phenolic and total flavonoid contents were determined using a spectrophotometric method. Total phenolic (343 ± 0.05 µg/mg) and flavonoid (220.5 ± 0.04 µg/mg) were indicated as gallic acid and quercetin equivalents respectively. The extract exhibited significant IC50 values in lipid peroxidation (IC50= 181.18 ± 0.65 µg/mL) and DPPH radical scavenging assays (IC50= 14.92 ± 0.032 µg/mL). The extract also partially protected 46966 plasmid DNA from free radical-mediated oxidative stress in a DNA damage inhibition assay and showed concentration-dependent antiproliferative effects. n-butanol extract of L. bonduelli is a rich source of natural antioxidants and anticancer agents.

Chemical Constituents, Anticancer and Anti-Proliferative Potential of Limonium Species: A Systematic Review

“…Of the 33 articles read in full, 16 were eligible for inclusion. One additional record was found during manual searches, resulting in 17 studies for synthesis as shown in Figure 1 [ 18 , 22 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 ]. See the list of the studies excluded after full-text reading in the Supplementary Materials (Board S3) .…”

“…The anti-proliferative activity of an n -butanol extract from aerial parts of Limonium bonduellei (T.Lestib.) Kuntze against two human cancer cell lines (HT-29 and HeLa) was evaluated by Amrani et al [ 36 ]. The extract showed a concentration-dependent anti-proliferative effect.…”

“…Low concentrations showed better activity against the HeLa cell line at 15 h and HT-29 cell line at 30 h, after treatment. The highest concentration of an n -butanol extract of L. bonduellei (250 μg/mL) showed the highest proliferation inhibition in all cell lines (92.6% in HT-29 and 98.9% in HeLa) [ 36 ].…”

“…In addition, several studies demonstrated the antioxidant potential of Limonium species associated with their high polyphenol content [ 18 , 36 , 65 ]. Overall, studies suggest that oxidative stress, chronic inflammation, and cancer are closely related [ 66 ].…”

Antioxidant activity and chemical constituents of Anthriscus vulgaris Bernh. (Apiaceae) from Algeria

“…Lipid peroxidation in egg vitellus is evaluated by the malondialdehyde assay (MDA) according to the methods described by Lizcano et al [20] and Amrani et al [21]. The fresh vitellus was homogenized in ice cold sodium phosphate buffer (20 mM, pH=7.4) to produce a 10% homogenate (v/v) and centrifuged at 4000 rpm for 20 min.…”