Cytotoxicity Assessment of a Poly(methyl methacrylate) Synthesized for the Direct Fabrication of Bone Tissues

Liñan, Lamia Zuñiga; Lima, Nádson Murilo Nascimento; Benatti, Carol; Xavier, Michelle da Cunha Abreu; Rodrigues, Ana Amélia; Manenti, Flavio; Jardini, André Luiz; Filho, Rubens Maciel; Gilioli, Rovílson

doi:10.1590/1678-4324-2018160526

Cited by 6 publications

(4 citation statements)

References 17 publications

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“…Therefore, both qualitative and quantitative cytotoxicity tests carried out in direct and indirect contact with L929 cells suggested that the investigated samples (PMMA/ZnO NPs and PMMA) did not cause cytotoxicity. The absence of cytotoxicity of our samples of PMMA is in accordance with published results [41]. In contrast, ZnO NPs are cytotoxic [19,20,42].…”

Section: Cytotoxicitysupporting

confidence: 93%

Microstructure Characterisation and Identification of the Mechanical and Functional Properties of a New PMMA-ZnO Composite

et al. 2020

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In this research work, we synthesised poly(methyl methacrylate) (PMMA) enriched with 2 wt.% zinc oxide nanoparticles (ZnO) through conventional heat polymerisation and characterised its microstructure. It was found that the distribution of ZnO nanoparticles was homogeneous through the volume of the PMMA. The mechanical testing of the PMMA-ZnO composite primarily included the determination of the compressive properties on real dentures, while density measurements were performed using a pycnometer. The testing of functional properties involved the identification of the colour of the new PMMA-ZnO composite, where pure PMMA acted as a control. In the second step, the PMMA-ZnO cytotoxicity assays were measured in vitro, which were shown to be similar to the control PMMA. Based on this, it could be concluded that the newly formed PMMA-ZnO composite did not induce direct or indirect cytotoxic effects in L929 cell cultures; therefore, according to ISO/DIN 10993-5:2009, this composite was categorised as non-cytotoxic.

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Section: Cytotoxicitysupporting

confidence: 93%

Microstructure Characterisation and Identification of the Mechanical and Functional Properties of a New PMMA-ZnO Composite

et al. 2020

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“…The results obtained in this assay shows an intense red fluorescence for positive toxicity control confirming the death of the cells present for both cell lines, and an intense and similar green fluorescence for negative toxicity control and for the cells that were in contact with the membranes (PLLA). These results can be compared with literature [17], where the results for 24 hours of contact of the cells in controls and in contact with material have a similar behavior. In both cases, the materials and the negative controls are intense green colored indicating live cells and the positive controls are red colored what indicates cell death.…”

Section: Discussionsupporting

confidence: 53%

Viability Assays of PLLA Fibrous Membranes Produced by Rotary Jet Spinning for Application in Tissue Engineering

Ciocca

Jardini

Cardoso

et al. 2019

Braz. arch. biol. technol.

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Tissue engineering suggests different forms to reconstruct tissues and organs. One of the ways is through the use of polymeric biomaterials such as poly(L-lactic acid) (PLLA). PLLA is a recognized material in tissue engineering due to its characteristics as biocompatibility and bioresorbability. In this work PLLA fibrous membranes were produced HIGHLIGHTS • PLLA fibrous membranes were produced by rotary jet spinning. • Membranes were subjected to preliminary in vitro assays to verify cytotoxicity. • Direct cytotoxicity assays performed with fibroblastic and osteoblastic cells. • PLLA membranes produced by rotary jet spinning showed promising results. Ciocca, B.E.; et al.

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“…Nevertheless, it was found that the photopolymers were highly toxic due to the presence of UFP, nano-sized particles less than 100 nm in diameter, known to be released from ABS materials. PMMA biocompatibility has been proven by results of a recent 72 h cytotoxicity assessment of samples, as determined through growth monitoring, adherence, and morphology of L-929 cells in which a similar ethanol-based protocol was used for sterilization [ 119 ]. However, the above-mentioned samples were not photopolymerized, unlike the custom-printed SLA-fabricated piece in the current study.…”

Section: Discussionmentioning

confidence: 99%

Three-Dimensional (3D) Printed Microneedles for Microencapsulated Cell Extrusion

et al. 2018

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Cell-hydrogel based therapies offer great promise for wound healing. The specific aim of this study was to assess the viability of human hepatocellular carcinoma (HepG2) cells immobilized in atomized alginate capsules (3.5% (w/v) alginate, d = 225 µm ± 24.5 µm) post-extrusion through a three-dimensional (3D) printed methacrylate-based custom hollow microneedle assembly (circular array of 13 conical frusta) fabricated using stereolithography. With a jetting reliability of 80%, the solvent-sterilized device with a root mean square roughness of 158 nm at the extrusion nozzle tip (d = 325 μm) was operated at a flowrate of 12 mL/min. There was no significant difference between the viability of the sheared and control samples for extrusion times of 2 h (p = 0.14, α = 0.05) and 24 h (p = 0.5, α = 0.05) post-atomization. Factoring the increase in extrusion yield from 21.2% to 56.4% attributed to hydrogel bioerosion quantifiable by a loss in resilience from 5470 (J/m3) to 3250 (J/m3), there was no significant difference in percentage relative payload (p = 0.2628, α = 0.05) when extrusion occurred 24 h (12.2 ± 4.9%) when compared to 2 h (9.9 ± 2.8%) post-atomization. Results from this paper highlight the feasibility of encapsulated cell extrusion, specifically protection from shear, through a hollow microneedle assembly reported for the first time in literature.

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Cytotoxicity Assessment of a Poly(methyl methacrylate) Synthesized for the Direct Fabrication of Bone Tissues

Cited by 6 publications

References 17 publications

Microstructure Characterisation and Identification of the Mechanical and Functional Properties of a New PMMA-ZnO Composite

Microstructure Characterisation and Identification of the Mechanical and Functional Properties of a New PMMA-ZnO Composite

Viability Assays of PLLA Fibrous Membranes Produced by Rotary Jet Spinning for Application in Tissue Engineering

Three-Dimensional (3D) Printed Microneedles for Microencapsulated Cell Extrusion

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