Degradation of monoaromatics by Bacillus pumilus MVSV3

Surendra, Sheeba Varma; Mahalingam, Brinda Lakshmi; Velan, M.

doi:10.1590/1678-4324-2017160319

Cited by 11 publications

(6 citation statements)

References 36 publications

(23 reference statements)

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“…The isolated bacterial strain, B. pumilus , could degrade dibenzothiophene and various metabolites [ 47 ]. Catechol 1,2 dioxygenase (C12D) synthesized by B. pumilus MVSV3 was found to effectively degrade hydrocarbons [ 48 ]. These analyses clearly revealed the involvement of biosurfactants and other gene products in hydrocarbon degradation.…”

Section: Resultsmentioning

confidence: 99%

Enhanced Production of Biosurfactant from Bacillus subtilis Strain Al-Dhabi-130 under Solid-State Fermentation Using Date Molasses from Saudi Arabia for Bioremediation of Crude-Oil-Contaminated Soils

Al-Dhabi

Esmail

Arasu

2020

IJERPH

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Crude oil and its derivatives are the most important pollutants in natural environments. Bioremediation of crude oil using bacteria has emerged as a green cleanup approach in recent years. In this study, biosurfactant-producing Bacillus subtilis strain Al-Dhabi-130 was isolated from the marine soil sediment. This organism was cultured in solid-state fermentation using agro-residues to produce cost-effective biosurfactants for the bioremediation of crude-oil contaminated environments. Date molasses improved biosurfactant production and were used for further optimization studies. The traditional “one-variable-at-a-time approach”, “two-level full factorial designs”, and a response surface methodology were used to optimize the concentrations of date molasses and nutrient supplements for surfactant production. The optimum bioprocess conditions were 79.3% (v/w) moisture, 34 h incubation period, and 8.3% (v/v) glucose in date molasses. To validate the quadratic model, the production of biosurfactant was performed in triplicate experiments, with yields of 74 mg/g substrate. These findings support the applications of date molasses for the production of biosurfactants by B. subtilis strain Al-Dhabi-130. Analytical experiments revealed that the bacterial strain degraded various aromatic hydrocarbons and n-alkanes within two weeks of culture with 1% crude oil. The crude biosurfactant produced by the B. subtilis strain Al-Dhabi-130 desorbed 89% of applied crude oil from the soil sample. To conclude, biosurfactant-producing bacterial strains can increase emulsification of crude oil and support the degradation of crude oil.

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Section: Resultsmentioning

confidence: 99%

Enhanced Production of Biosurfactant from Bacillus subtilis Strain Al-Dhabi-130 under Solid-State Fermentation Using Date Molasses from Saudi Arabia for Bioremediation of Crude-Oil-Contaminated Soils

Al-Dhabi

Esmail

Arasu

2020

IJERPH

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“…Only a few Bacillus strains were reported as BTEX degraders, for example, B. subtilis DM-04 (able to degrade benzene, toluene and m- xylene) 50 , B. stratosphericus FLU-5 (capable of growing on toluene, ethylbenzene, o- xylene, m- xylene and p- xylene) 51 and B. cereus ATHH39 (able to degrade toluene) 52 . Thus far, only Bacillus pumilus MVSV3 has been reported for its mono-oxidation (for toluene and ethylbenzene) and di-oxidation (for benzene and o- xylene) 22 . In this study, biodegradation pathways of all six BTEX compounds in B. amyloliquefaciens were proposed for the first time (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Most Bacillus were, however, reported as a potent crude oil degrader 21 . Although Bacillus pumilus MVSV3 was reported to completely degrade 150 mg/L (or ppm) of BTEX within 48 h 22 , B. pumilus has been reported to cause a cutaneous infection similar to an anthrax lesion 23 . Therefore, the strain may not be suitable for an application in an open system .…”

Section: Introductionmentioning

confidence: 99%

BTEX biodegradation by Bacillus amyloliquefaciens subsp. plantarum W1 and its proposed BTEX biodegradation pathways

Wongbunmak

Khiawjan

Suphantharika

et al. 2020

Sci Rep

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Benzene, toluene, ethylbenzene and (p-, m- and o-) xylene (BTEX) are classified as main pollutants by several environmental protection agencies. In this study, a non-pathogenic, Gram-positive rod-shape bacterium with an ability to degrade all six BTEX compounds, employed as an individual substrate or as a mixture, was isolated. The bacterial isolate was identified as Bacillus amyloliquefaciens subsp. plantarum strain W1. An overall BTEX biodegradation (as individual substrates) by strain W1 could be ranked as: toluene > benzene, ethylbenzene, p-xylene > m-xylene > o-xylene. When presented in a BTEX mixture, m-xylene and o-xylene biodegradation was slightly improved suggesting an induction effect by other BTEX components. BTEX biodegradation pathways of strain W1 were proposed based on analyses of its metabolic intermediates identified by LC–MS/MS. Detected activity of several putative monooxygenases and dioxygenases suggested the versatility of strain W1. Thus far, this is the first report of biodegradation pathways for all of the six BTEX compounds by a unique bacterium of the genus Bacillus. Moreover, B. amyloliquefaciens subsp. plantarum W1 could be a good candidate for an in situ bioremediation considering its Generally Recognized as Safe (GRAS) status and a possibility to serve as a plant growth-promoting rhizobacterium (PGPR).

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“…Many chemical and physical methods can be used for degradation and breakup into nontoxic products. But, use of microorganism is considered to be the best suitable way for the degradation of BTEX [6,34,35]. Use of microorganisms and their enzymes for degradation can overcome the difficulties with other conventional methods.…”

Section: Resultsmentioning

confidence: 99%

Isolation of Novel Toluene Degrading Bacteria from Waste Water Treatment Plants and Determination of their Toluene Tolerance and other Biotechnological Potential

Poyraz¹

2020

Pol. J. Environ. Stud.

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Toluene is an aromatic hydrocarbon and spreads to the environment by the release of petroleum products, agricultural and industrial activities and toluene can cause serious social and health problems. Since the removal in toluene is considered an important environmental issue, there is an increasing interest of toluene biodegradation. In this comprehensive study, novel toluene degrading bacteria were isolated and identified both from municipal and industrial wastewater treatment plants. Isolates' toluene tolerance, enzyme production, benzene, ethyl benzene, xylene and phenol degradation potentials and biofilm formation were examined. Totally 109 bacterial isolates were obtained and most of them were determined as belong to Stenotrophomonas, Acinetobacter and Pseudomonas species.Isolates' toluene tolerance, enzyme production, benzene, ethyl benzene, xylene and phenol degradation potentials and biofilm formation were examined. Most of isolates showed lipase, DNase and protease activity. Most of isolates showed growth in 300 mg/l -1 concentrations of benzene, ethyl benzene, xylene and phenol. Strains E4T16, 3AT2 and 3ET5 showed maximum growth in high toluene concentrations and also strain E4T16 showed growth up to 2100mg/l -1 toluene. Examination of biofilm formation at different toluene concentrations at the end of the 96 hours incubation revealed that strains E4T16, 4ET21 and 3ET5 had highly biofilm production potential. In conclusion, these bacteria and results could be considered as a powerful new approach for the removal of hydrocarbons from wastewater.

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Degradation of monoaromatics by Bacillus pumilus MVSV3

Cited by 11 publications

References 36 publications

Enhanced Production of Biosurfactant from Bacillus subtilis Strain Al-Dhabi-130 under Solid-State Fermentation Using Date Molasses from Saudi Arabia for Bioremediation of Crude-Oil-Contaminated Soils

Enhanced Production of Biosurfactant from Bacillus subtilis Strain Al-Dhabi-130 under Solid-State Fermentation Using Date Molasses from Saudi Arabia for Bioremediation of Crude-Oil-Contaminated Soils

BTEX biodegradation by Bacillus amyloliquefaciens subsp. plantarum W1 and its proposed BTEX biodegradation pathways

Isolation of Novel Toluene Degrading Bacteria from Waste Water Treatment Plants and Determination of their Toluene Tolerance and other Biotechnological Potential

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