Apoptosis and Histopathology of the Heart after Renal Ischemia-Reperfusion in Male Rat Running title: Ischemia-Reperfusion Injury

Alihemmati, Alireza; Yousefi, Hadi; Ahmadiasl, Nasser; Habibi, Parisa

doi:10.1590/1678-4324-2017160244

Cited by 3 publications

(3 citation statements)

References 27 publications

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“…It is reported that the renal ischemia reperfusion could dramatically decrease expression of Bcl-2 and increase expression of Bax in the brain tissue of I/R rats [54]. Although reports that male rats subjected 45 min of kidney ischemia indicate increasing connective tissue in the heart muscles myofibrils as a sign of tissue injury and apoptosis of myofibrils after 24 h reperfusion following kidney I/R [55]. Losing mitochondrial outer membrane could release cytochrome C and other mitochondrial proteins in cytosol which can activate apoptotic caspases such as the main executive protease: Caspase 3 [56].…”

Section: Discussionmentioning

confidence: 99%

Prazosin Treatment Protects Brain and Heart by Diminishing Oxidative Stress and Apoptotic Pathways After Renal Ischemia Reperfusion

et al. 2022

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Acute kidney injury (AKI) is a major medical challenge caused from renal ischemia-reperfusion (IR) injury connected with different cellular events in other distant organs. Renal IR-related oxidative stress and inflammation followed by cell apoptosis play a crucial role in IR-induced distant organ pathological damages. Prazosin has shown protective effects against IR-injuries. Thus, the current study intended to investigate the possible protective role of prazosin against the consequents of renal IR in the heart and brain tissues. To reach this goal, rats were randomly divided into 3 groups (n=7): Sham, IR and prazosin pretreatment-IR animals (1 mg/kg intraperitoneally injection of prazosin 45 min before IR induction). After 6 h reperfusion, lipid peroxidation and antioxidant markers levels were evaluated in the both, brain and heart tissue. Moreover, apoptotic pathway in the heart and brain tissues were assessed by western blotting. Accordingly, prazosin pretreatment in IR model rats could significantly increase the antioxidant capacity and attenuate apoptotic pathways by increasing the bcl-2 levels and decreasing the expression of Bax and caspase 3 enzymes (P<0.05). Thus, prazosin suppressed cellular damages of heart and brain tissues post kidney IR by anti-oxidative and anti-apoptotic effects, which suggests the plausible use of prazosin in improving the clinical outcomes during AKI after further investigations.

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Section: Discussionmentioning

confidence: 99%

Prazosin Treatment Protects Brain and Heart by Diminishing Oxidative Stress and Apoptotic Pathways After Renal Ischemia Reperfusion

et al. 2022

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“…Assessment is based on observations of 10 view fields with ×400 magnification. Histological changes scored on a 4-point scale: (−) none, (+) mild, (++) moderate, and (+++) severe damage [11]. The morphological measurement was…”

Section: Histopathological Assessmentmentioning

confidence: 99%

Effect of Simvastatin on Histopathology of the Heart After 5/6 Subtotal Nephrectomy

Cahyawati

2019

Int J App Pharm

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Objective: This study aims to assess the condition of cardiac histopathology through hematoxylin-eosin staining in 5/6 subtotal nephrectomyconditions.Methods: Fifteen male Swiss mice aged 3–5 months will be grouped into 3 treatment groups, namely the nephrectomy group (JSN, n=5), shamoperation (JSO, n=5), and simvastatin 20 mg/kg body weight (JSIM, n=5). The histopathology of the heart will be assessed blindly. Severity is assessedbased on scoring using a scale (−) no damage, (+) mild, (++) medium, and (+++) heavy. Assessment of severity refers to the irregularity of the heartmuscle, increased amount of connective tissue, myofibril hypertrophy, myofibril swelling, sarcoplasmic fragmentation, sarcoplasmic vacuolization,bleeding in a myofibril, myofibril degeneration, cardiomyocyte damage, and the presence of acidophilic cytoplasm.Results: The results showed no morphological changes in heart muscle tissue in the JSO group except for fragmentation and vacuolization in minimalamounts of sarcoplasm (+), whereas in the JSN and JSIM groups, there was moderate damage to sarcoplasm (++) and minimal changes in myofibrils(hypertrophy and bleeding) (+). The JSN group also found severe damage (+++) to the irregularity of the heart muscle, whereas in JSIM, only moderatedamage was found (++) to the irregularity of the heart muscle.Conclusion: Simvastatin seems to be able to correct the irregularity of the heart muscle in the condition of 5/6 subtotal nephrectomy.

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“…11 AKI-induced cardiac injury is mostly mediated by activation of the sympathetic nervous system (SNS) or unstable renin–angiotensin–aldosterone system (RAAS). 12 Cardiomyopathy and cell apoptosis are elevated in the heart tissue 24 h after IRI 13 and defective cardiac mitochondria may be important in heart injury of AKI patients. 14 Loss of memory and neural cell apoptosis along with blood-brain barrier (BBB) disruption were found during bilateral renal I/R as a result of kidney-brain crosstalk.…”

Section: Introductionmentioning

confidence: 99%

Protective effects of Gamma Oryzanol on distant organs after kidney ischemia-reperfusion in rats: A focus on liver protection

et al. 2020

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Background: Acute kidney injury (AKI) is the main clinical concern resulted from ischemia-reperfusion injury (IRI). Ample clinical data indicates that AKI is associated with distant organ dysfunctions and poor patients’ outcomes. Oxidative stress and inflammation have a critical role in the pathogenesis of organ injuries following IRI. The objectives of this study were to determine the impact of Gamma Oryzanol (GO), extracted from rice bran oil, on distant organs in rats after IRI. Methods: Twelve out of 24 Wistar rats were treated by one dosage of GO (100mg/kg) 1 h before I/R induction through both oral gavage and intraperitoneal injection. Then, the AKI model rats were induced by IRI. Oxidative stress and antioxidant protein levels were assessed in the brain, heart, and liver tissues in the experimental groups. Furthermore, the effects of GO on IRI-induced liver dysfunction, apoptosis, and inflammation were measured by Western blot. Results: GO pretreatment could significantly restore the levels and activity of antioxidant proteins in the brain, heart, and liver tissues (P < 0.05). Moreover, GO pretreatment could decrease the inflammatory cytokine (IL-1, IL-6, and TNF-α) in the liver (P < 0.01). By reducing Bax/Bcl-2 ratio and down-regulating caspase-3, GO could significantly diminish apoptosis in the liver tissue after the kidney I/R (P < 0.01). Additionally, GO could significantly diminish the deterioration of liver function in the kidney I/R model. Conclusion: GO protects distant organs against renal IRI-induced oxidative stress. Furthermore, it ameliorates liver function and remarkably exerts anti-oxidative, anti-inflammatory, and anti-apoptotic roles in the liver as an important detoxifying organ.

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Apoptosis and Histopathology of the Heart after Renal Ischemia-Reperfusion in Male Rat Running title: Ischemia-Reperfusion Injury

Abstract: Ischemia-reperfusion injury was seen in strokes, myocardial infarctions, acute kidney injury, mesenteric ischemia

Cited by 3 publications

References 27 publications

Prazosin Treatment Protects Brain and Heart by Diminishing Oxidative Stress and Apoptotic Pathways After Renal Ischemia Reperfusion

Prazosin Treatment Protects Brain and Heart by Diminishing Oxidative Stress and Apoptotic Pathways After Renal Ischemia Reperfusion

Effect of Simvastatin on Histopathology of the Heart After 5/6 Subtotal Nephrectomy

Protective effects of Gamma Oryzanol on distant organs after kidney ischemia-reperfusion in rats: A focus on liver protection

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