Expression analysis of three immune genes Interferon-gamma, Mx and Interferon regulatory factor-1 of Japanese flounder ( Paralichthys olivaceus )

Abstract

IFN-γ (Interferon-gamma), Mx and IRF-1 (Interferon regulatory factor-1) are main immune-related genes and they play important roles in the innate immune system of vertebrates. In this study, the expression level of the three immune-related genes in twelve tissues of normal adult Japanese flounder was detected using semi-quantitative RT-PCR. Thirteen time points (3h, 6h, 9h, 12h, 24h, 36h, 48h, 60h, 72h, 84h, 96h, 108h, 120h)

Molecular Mechanism Involved in Carotenoid Metabolism in Post-Smolt Atlantic Salmon: Astaxanthin Metabolism During Flesh Pigmentation and Its Antioxidant Properties

“…The Ax supplementation could therefore reflect a positive effect as the lower expression of the gbp1 might decrease inflammation through reduced IFNg production, which seems to be also correlated with the lower expression of the transcription factor IRF1 closely related to ifng expression. These genes encode proteins that have already been described as important key molecules in immune response (Zhang et al 2017). Also closely related to IFNs, MHC class II genes are up-regulated by IFNg by activation of the class II transactivator factor (CIITA) (Jørgensen et al 2007).…”