In this study, ethanol (EtOH), methanol (MeOH) and dichloromethane (DCM) extracts of the plant samples were obtained. The total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI) and antimicrobial activity of the different extracts of Satureja hortensis L. collected from Erzincan (Turkey) province were determined. Rel Assay Diagnostics kits are used to determine TAS, TOS and OSI values. Antimicrobial activity was determined with 9 different bacteria and fungi strain (Staphylococcus aureus, Staphylococcus aureus MRSA, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, Candida albicans, Candida krusei and Candida glabrata) using modified agar dilution method. The plant extracts in this study were antimicrobially effective between 25-800 µg/mL concentrations level on 9 different microorganisms strain. Also the plant extracts showed the highest activity against A. baumannii. As a result, S. hortensis plant from Erzincan province could be a good natural antioxidant and antimicrobial source with its high antioxidant and low oxidant capacity