Isolation, Identification and Molecular Characterization of Highly Pathogenic Newcastle Disease Virus From Field Outbreaks

Ashraf, Asma; Shah, Muhammad Arif; Habib, Md. Ahasan; Hussain, Mujahid; Mahboob, Shahid; Al‐Ghanim, Khalid A.

doi:10.1590/1678-4324-2016160301

Cited by 5 publications

(3 citation statements)

References 20 publications

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“…Newcastle disease virus (NDV) is a non-segmented, single-stranded, negative-sense, enveloped RNA virus belonging to the Paramyxoviridae family, the Paramyxovirinae subfamily and the genus Avula virus (Ashraf et al, 2016). The virus exists in ten serotypes APMV-1 to 10 and NDV is synonymous with avian paramyxovirus type 1 (APMV-1).…”

Section: Etiology Of Ndvmentioning

confidence: 99%

Status updates of Newcastle disease and amelioration effects of medicinal plants against Newcastle disease virus: A review

Ashraf¹,

Mahboob²,

Andleeb³

et al. 2018

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Recently, medicinal plants are achieving great interest because of their use in ethno medicine treatment of different common diseases and also other medicinal assertions are now reinforced by comprehensive scientific evidence. Almost 82 research articles and abstracts published, so far, were screened for evaluating antiviral efficiency of various plant samples and 23 different plants were found to be traditionally used against Newcastle disease (ND). ND is a most transmissible viral disease of avian species caused by virulent strain of Avula virus from the Paramyxoviridae family. The first epidemic of ND was perceived in Java, Indonesia and England in year 1926. ND causes great economic loses to the commercial poultry farmers around the world. Medicinal plants are traditionally used in the control of viral or other diseases and infections. Plants have been found useful in treating many microbial diseases in man and animals caused by bacteria and viruses. The ability to synthesize compounds retaining antiviral potential by secondary metabolism makes plants a vital source of pharmaceutical and therapeutic products, which can reduce chemotherapeutic load in birds. Current studies signify that the natural products posses a rich potential source of new antiviral compounds. Further ethnobotanical studies and laboratory investigations are established to identify species having potential to improve ND control.

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Section: Etiology Of Ndvmentioning

confidence: 99%

Status updates of Newcastle disease and amelioration effects of medicinal plants against Newcastle disease virus: A review

Ashraf¹,

Mahboob²,

Andleeb³

et al. 2018

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“…Moreover, a very large amount of biomass is needed for each dose of the vaccine which makes their products extremely difficult. Although live experimental Active Immunization with Pasteurella multocida Lysate Elicits Antibody that Protects Rabbits against Virulent Pasteurella.... vaccines have been shown to promote heterologous and homologous protections, they carry unknown attenuation markers and in some cases, have been associated with disease outbreaks (Ashraf, 2014;Ahmad et al 2014). Cross protection has been achieved with bacterins (Glisson et al, 1993;Sarangi et al, 2014)) as well as with OMP's extracts from P. multocida grown in iron-depleted mediums.…”

Section: Introductionmentioning

confidence: 99%

Active Immunization with Pasteurella multocida Lysate Elicits Antibody that Protects Rabbits against Virulent Pasteurella multocida and Protects Mice by Passive Immunization

Durairajan

Verma

Prajapati

et al. 2021

IJAR

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Background: Pasteurellosis is an economically important disease of livestock worldwide and vaccination is effective mean to control the disease outbreaks. One of alternative method for vaccine preparation could be the use of lytic phages as bacterial inactivating agents and employing “phage lysate immunogen” as an immunizing agent. Therefore, the study was undertaken to explore the protective immune response of novel phage lysate vaccine against Pasteurella multocida. Methods: A phage lysate vaccine of Pasteurella multocida was prepared using isolated lytic phage and subjected to vaccine response study in rabbits. In this experiment, two mouse fixed-dose of lysate was used and the prophylactic efficacies of lysates were evaluated in rabbits by passive mouse protection test (PMPT), western blotting and direct virulent challenge.Result: Passive transfer of antibodies to mice using post-immunization sera of rabbits protected the animals against the challenge with A: 1 strain (75%) and B: 2 strain (50%). In western blotting, a total number of 4 bands were observed in the region between 130 kDa to 25 kDa in the protein of the phage lysate. The study suggested the significance increase in humoral immunity by phage lysate in murine Pasteurellosis.

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“…ND viruses a negative sense, non-segmented, single- stranded encapsulated RNA virus from family paramyxoviridae (Ashraf et al, 2016). ND is prevalent to many regions of world.…”

Section: Introductionmentioning

confidence: 99%

Analysis of bioactive composites and antiviral activity of Iresine herbstii extracts against Newcastle disease virus in ovo

Andleeb

Ashraf

Muzammil

et al. 2020

Saudi Journal of Biological Sciences

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The study was implemented to actuate the qualitative and quantitative phyto constituents of Iresine herbstii extracts and its antiviral efficacy against avian ND virus. Among four tested solvents, the ethanolic extract of Iresine herbstii revealed the presence of highest quantity of all tested phytochemicals while petroleum ether extract showed the least. Folin-Ciocalteu method assessed the range of TPC extended from 81.01 ± 0.67 to 126.35 ± 0.45 µg GAE/mg. Acetonic extract showed the highest amount among all extracts and petroleum ether possessed the lower quantity. TFC ranged from 54.37 ± 0.45 to 88.12 ± 0.26 µg QE/mg followed by colorimetric method. From all extract ethanolic extract showed highest quantity and petroleum ether revealed the lower. HPLC analysis of ethanolic extract of I. herbstii confesses the presence six bioactive components by using the HP5-MS column. To check the antiviral potential of plants, different prepared treatments of plant extract and live virus were inoculated at 9 days old SPF embryonated chicken eggs. Results exposed that all plant extracts produce antiviral activity against NDV in ovo according to their potential and phytochemical profile. The highest survival rate was observed in the ethanolic extract at 400 µg/mL and acetonic extract at 300 µg/mL as it controls the NDV activity completely, evidence of absence of embryo death and HA titre. Dichloromethane and petroleum ether could not inhibit the virus completely. 600 µg/mL concentration was proved as toxic in all extracts except petroleum ether extract which showed a dose dependent pattern.

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Isolation, Identification and Molecular Characterization of Highly Pathogenic Newcastle Disease Virus From Field Outbreaks

Abstract: Newcastle disease (ND) is a major infectious disease of the poultry caused by a virulent strain of Avian

Cited by 5 publications

References 20 publications

Status updates of Newcastle disease and amelioration effects of medicinal plants against Newcastle disease virus: A review

Status updates of Newcastle disease and amelioration effects of medicinal plants against Newcastle disease virus: A review

Active Immunization with Pasteurella multocida Lysate Elicits Antibody that Protects Rabbits against Virulent Pasteurella multocida and Protects Mice by Passive Immunization

Analysis of bioactive composites and antiviral activity of Iresine herbstii extracts against Newcastle disease virus in ovo

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