Clotting and fibrinogenolysis inhibition by essential oils from species of the Asteraceae family

Miranda, Cíntia Alvarenga Santos Fraga de; Cardoso, María das Graças; Marcussi, Silvana; Teixeira, Maria Luisa

doi:10.1590/1678-4324-2016150775

Cited by 9 publications

(8 citation statements)

References 30 publications

(35 reference statements)

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“…The only aromatic compound was p-cymene, and all of them are monoterpenes, also known as C10 compounds. Regarding the clotting time, similar results were observed by Miranda and collaborators [8] when evaluating the performance of the essential oils from the Asteraceae family plants. In their study, all EOs evaluated increased the time to form clots when compared to the same venoms tested in our study.…”

Section: Comet Assaysupporting

confidence: 82%

“…Fibrinogenolytic activities were evaluated according to Miranda and collaborators [8]. The first is the fragmentation profile analysis of fibrinogen molecules in polyacrylamide gel electrophoresis under reducing conditions (SDS-PAGE).…”

Section: Thrombolytic Fibrinogenolytic Clotting and Hemolysis Testsmentioning

confidence: 99%

“…In the present study, an EO dosage 24 times smaller than the one evaluated by Miranda and collaborators [8]. was tested to obtain partial or gradual enzymatic inhibition.…”

Section: Comet Assaymentioning

confidence: 99%

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Eucalyptus grandis and Eucalyptus microcorys Essential Oils: in vitro Modulation of Hemostasis and Genotoxic Activity

Espósito

César

Brandão

et al. 2021

Braz. arch. biol. technol.

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The genus Eucalyptus present high content of essential oil (EO). This study evaluated the pharmacological properties of Eucalyptus grandis (EG) and Eucalyptus microcorys (EM) EOs. The major component in both EOs was 1,8-Cineole. Both essential oils prevented thrombus dissolution and reduced clotting, hemolysis, and genotoxicity induced by snake venoms. 50% (EM) and 73% (EG) were the greatest inhibitions obtained in the thrombolytic assay -thrombolysis induced by B. moojeni venom. Increases in clotting time were also observed, with values considered significant between 10-27 seconds. Lysis values 50% lower than the negative control were observed in both EOs. The EOs also protected fibrinogenolysis induced by snake venom. EM EO was more effective in reducing venom-induced DNA fragmentation in the comet assay, with arbitrary unit values 66.15% lower than the positive control. These oils present wide application potential considering the pharmacological properties observed in this study.

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Section: Comet Assaysupporting

confidence: 82%

Section: Thrombolytic Fibrinogenolytic Clotting and Hemolysis Testsmentioning

confidence: 99%

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Eucalyptus grandis and Eucalyptus microcorys Essential Oils: in vitro Modulation of Hemostasis and Genotoxic Activity

Espósito

César

Brandão

et al. 2021

Braz. arch. biol. technol.

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“…In this research conducted in western Kenya, GC-MS analysis of essential oils from T. diversifolia showed that they constituted 54% monoterpenes and 46% sesquiterpenoids. Miranda et al (2016) confirm that limonene is one of the major constituents of essential oils. The findings of this research are in agreement with previous research findings.…”

Section: Fatty Alcoholmentioning

confidence: 60%

Contact toxicity of Essential Oils from Tithonia diversifolia against Aphis gosypii, Thrips tabaci and Bemisia tabaci

2022

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Plants and microbes provide naturally occurring chemicals that are often used for the control of pests all around the world. Biopesticides have been used as a comparatively safer replacement for synthetic pesticides for the past five decades. This study determined the contact toxicity of essential oils from the Tithonia diversifolia plant against Thrips tabaci, Bemisia tabaci, and Aphis gosypii. The essential oils (Eos) were extracted from dry leaves by hydrodistillation using the Clavenger apparatus for 8 hours. GC-MS was utilised to analyse the qualitative and quantitative composition of essential oils, whereas ATR-FTIR was employed to determine the functional groups. 3-Carene was the most abundant compound ion in the Eos. T. tabaci, B. tabaci, and A. gosypii were used in the bioassay of the crude extracts. T. tabaci, B. tabaci, and A. gosypii were tested for contact toxicity against mixed-sex adult pests. Five distinct concentrations were made, each of which was repeated five times. Permethrin, a commercial chemical pesticide was utilised as a positive control and acetone were used as a negative control. The essential oils’ LD50 was calculated using SPSS version 26.0 Probit analysis. After 24 hours, the pests’ response to the treatments was assessed using a blunt instrument probe, and those that did not respond were counted as dead. Essential oils from T. diversifolia had the lowest LD50 against T. tabaci, with a value of 0.085 µL. This suggests that T. diversifolia could be employed as a T. tabaci contact toxicant. (P < 0.05, α = 0.05) The outcomes were statistically significant. As a result, essential oils from T. diversifolia can be employed as a biopesticide against T. tabaci as a contact toxicant.

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“…The clotting test was performed with citrated human plasma, according to Miranda et al (2016), with modifications in the venoms used as controls and the volumes of oils evaluated. The difference of 10 seconds between times observed in controls and treatments was considered significant once the prothrombin activation occurs between 10 to 14 seconds.…”

Section: Thrombolytic and Clotting Activitiesmentioning

confidence: 99%

Thrombolysis, clotting, and genotoxic activities modulated by essential oils extracted from Lippia alba

Espósito

Trento

Braga

et al. 2022

Braz. J. Pharm. Sci.

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The composition and pharmacological properties of Lippia alba (Mill.) (L. alba) (Verbenaceae) flower and leaf essential oils (EO) were determined in this study. The major constituents in the flower EO were geranial (49.83%) and neral (32.75%), and in the leaf EO were geranial (38.06%), neral (31.02%), and limonene (18.03%). Flower EO inhibited thrombolysis induced by Bothrops moojeni (B. moojeni) and Lachesis muta muta (L. muta muta) venoms (0.05-1.2 µL mL -1 ). When tested against L. muta muta venom, the protective effect was smaller in both EO. The EOs prolonged the clotting time induced by L. muta muta venom and a procoagulant effect was observed on B. moojeni. In the comet assay, the flower EO presented anti-genotoxic action (damage frequency of only 11.6 -34.9%) against the L. muta muta venom. The positive control (Doxorubicin) and the venom alone presented a damage frequency of 80.3% and 70.7%, respectively. The flower EO protected DNA from damage induced by L. muta muta venom. L. alba leaf and flower EOs presented anti-genotoxic action.

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Clotting and fibrinogenolysis inhibition by essential oils from species of the Asteraceae family

Abstract: ABSTRACT

Cited by 9 publications

References 30 publications

Eucalyptus grandis and Eucalyptus microcorys Essential Oils: in vitro Modulation of Hemostasis and Genotoxic Activity

Eucalyptus grandis and Eucalyptus microcorys Essential Oils: in vitro Modulation of Hemostasis and Genotoxic Activity

Contact toxicity of Essential Oils from Tithonia diversifolia against Aphis gosypii, Thrips tabaci and Bemisia tabaci

Thrombolysis, clotting, and genotoxic activities modulated by essential oils extracted from Lippia alba

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