Micropropagation of Endangered and Decorative Species Dianthus pinifolius Sibth. et Sm.

Abstract

The aim of this study was to establish an efficient protocol for the in vitro propagation of the endangered, endemic and decorative species Dianthus pinifolius Sibth. et Sm. The effects of different concentrations of 6-benzylaminopurine (BAP) and naphtalenacetic acid (NAA), and different explant types (single-node cuttings, terminal buds and shoot cuttings) on shoot multiplication were examined on Murashige and Skoog (MS) and halfstrength MS media. The best results were obtained for shoot cuttings on the MS medium supplemented with 0.5 mg/L BAP and 0.1 mg/L NAA, achieving a maximum rate of regeneration (100%) and a total of 15.4 newlydeveloped shoots per explant. The highest rooting rate (96.7%) was obtained on MS medium containing 1 mg/L NAA, while the acclimatization of the microplants obtained to ex vitro conditions was successful (88.9%).

An efficient in vitro propagation protocol of Dianthus giganteiformis Borbas subsp. kladovanus (Degen) Soo

“…The seeds successfully germinated in the sterile culture with a high germination percentage (88%), which is an important factor for preserving a population variability after propagation. Although this value is lower than the germination rate achieved by D. pinifolius -92% or D. carthusianorum -95% (Marković et al 2016; Mu szyń ska & H a n u s -Fa j e rs ka , 2017), it is significantly higher than the germination percentage in vitro of some other Dianthus species, such as D. glacialis -31%, D. giganteus ssp. croaticus -42%, D. ingoldbyi -65%, or D. henteri -75% (Colombo et al, 2004;Radojević et al, 2010;Pop & Pamfil, 2011;Arda et al, 2016).…”

“…The impact of the concentration of MS salts is not significant, but generally longer shoots develop on MS media. The impact of the concentration of MS salts on shoot length was recorded for D. serotinus and D. pinifolius (Marković et al, 2013(Marković et al, , 2016, with longer shoots developed on full strength MS media. Nevertheless, half strength MS media can have a favourable effect on shoot regeneration (D e s i l et s et al.…”

“…The concentration of MS salts significantly influenced rooting, and the percentage was considerably higher on half strength MS media (table 3), which was also obtained for D. mainensis (Erst et al 2014). However, during the micropropagation of D. pinifolius, the rooting percentage was higher on MS media than on half-strength MS media (Marković et al 2016), and, in some cases (e.g. D. nardiformis), the concentration of MS salts (MS, 1/2MS or 1/4MS) had no significant effect on the percentage of rooting (Holobiuc et al 2010).…”

“…Since micropropagation is an important method for ex situ and in situ conservation of endangered taxa (Fay, 1992; Pe n c e , 1999), numerous research papers, investigating the possibilities of successful micropropagation of the endangered or endemic Dianthus spp. in the Balkans and Romania, have been published (C r i s t e a , 2010; J a rd a et al, Cristea et al, 2013aCristea et al, , 2013bCristea et al, , 2014Tsoktouri dis et al 2013;Jarda et al, 2014;Marković et al, 2016). Micropropagation protocols obtained in those studies for more than 20 Dianthus taxa differ depending on the species or even a genotype of the same species.…”

In Vitro Regeneration and ISSR-Based Genetic Fidelity Analysis of Orthosiphon stamineus Benth

“…Other than that, one of the main aims of mass propagation of plants through tissue culture is to generate clonal plantlets with superior quality. Thus, ensuring the genetic uniformity of tissue culture-raised plants is important for mass production of elite crops or cultivars such as rice [44], cotton [45], and sugarcane [46], as well as for conservation of endangered plant species [47][48][49]. Furthermore, plant tissue culture also allows for uniform production of clonal plants to be used as plant factories for production of novel and important bioactive compounds with medicinal properties [50,51].…”