Identification of subclinical mastitis caused by Mycoplasma spp. from screenings of bulk tanks

Joaquim, Sâmea Fernandes; Guimarães, F. F.; Salina, A.; Junqueira, N. B.; Gomes, Eduardo Nardini; Langoni, Hélio

doi:10.1590/1678-4162-9982

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…The detection of 16S rRNA in bacteria has been made possible by various primer systems, although each has a different focus on clinical samples and pathogens. Many studies in BM diagnostics with the help of universal 16S bacterial rRNA-based nested PCR methods were published by Joaquim et al [ 25 ] with the help of universal 16S bacterial rRNA-based nested PCR methods.…”

Section: Discussionmentioning

confidence: 99%

Expressions of IL4, IL10, and IFN"&#".ord($0).";""&#".ord($0).";" cytokines genes during bacterial mastitis

Faaz¹,

Abdullah²

2022

J Adv Vet Anim Res

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Objective: Many bacteria are involved in causing mastitis in dairy cows. Perfect identification of bacteria is crucial for the appropriate choice of drug for treatment. This study aims to find out the various bacteria that cause mastitis through the 16S ribosomal ribonucleic acid ( 16S rRNA ) gene. Materials and Methods: A total of 150 mastitis somatic cell samples were tested with bacterial nested polymerase chain reaction (PCR) universal primers, targeting the 16S rRNA gene. The primers had both Gram-positive and Gram-negative bacterial specificities. Inflammatory cytokine interleukin (IL-10), IL-4, and interferon-gamma (IFNγ) expression genes were measured and compared in mastitis-free and mastitis-affected animals. Results: Based on the PCR, 70 (46.7%) samples showed positive results. The expression of the IL-10 gene was significantly higher ( p < 0.001) in mastitis-affected cows than noninfected animals. Compared to cows diagnosed with clinical mastitis, the IL-4 and IFNγ genes were expressed more strongly in healthy cows ( p > < 0.0001). Conclusion: Mastitis has been linked to both Gram-positive and Gram-negative bacteria. These genes are strong predictors of mastitis in the states analyzed, as evidenced by the differential expression in mastitis and healthy conditions of the IL-4, IL-10, and IFNγ genes. The genes examined here and others will be the subject of additional research.

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Section: Discussionmentioning

confidence: 99%

Expressions of IL4, IL10, and IFN"&#".ord($0).";""&#".ord($0).";" cytokines genes during bacterial mastitis

Faaz¹,

Abdullah²

2022

J Adv Vet Anim Res

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“…Clinical signs in cows such as arthritis and pneumonia may suggest the presence of M. bovis in the herd [12]. In Brazil, the presence of the pathogen has been detected in dairy herds, in cases of clinical mastitis, and in bulk tanks [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

The Efficiency of Centrifugation in the Detection of Mollicutes in Bovine Milk

Salina,

Lopes,

Barone

et al. 2023

Ruminants

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Mastitis is one of the main diseases that affects dairy cattle. It is characterized by a multifactorial disease with multiple etiologies including microorganisms such as bacteria, viruses, fungi, yeasts, and algae. Mastitis caused by Mycoplasma spp. results in a contagious nature of infection and has been studied much in Brazil. The objectives of this research were (1) to investigate in clinical mastitis milk samples, by conventional PCR, the presence of the following mycoplasma species: Mycoplasma (M.) bovis, M. bovirhinis, M. bovigenitalium, M. californicum, and M. alkalescens and (2) compare the PCR Mollicutes detection rates using previous concentration and homogenization steps of the milk samples. Of the 993 clinical mastitis milk samples analyzed, 13.7% (136/993) and 8.0% (80/993) were positive for the Mollicutes class after concentration and homogenization, respectively, and all of them were PCR-negative for the five species investigated. Of the 70 expansion milk tanks analyzed, all of them were PCR-negative for the Mollicutes class. The importance of the previous concentration of milk in the routine of molecular analysis was evidenced when compared to the results obtained only with homogenization.

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“…Thus, it is difficult to compare the occurrence of the most prevalent species of these agents in the country (SALINA et al, 2020). The first isolation of M. bovis from bovine milk occurred in a mastitis outbreak in Paraná (METTIFOGO et al, 1996) and, since then, few studies have been conducted, restricting the identification of the agent in herds in Paraná, São Paulo, Minas Gerais and Goiás, with occurence in animals ranging from 1 to 3% (PRETTO et al, 2001;FRANCHESCHINI et al, 2008;JUNQUEIRA, 2017;JOAQUIM et al, 2018;MANZI et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Risk factors associated with intramammary colonization with Mollicutes in dairy cattle from Southeast Brazil

et al. 2021

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Bacteria of Mollicutes Class are associated with intramammary infection and decrease in milk production. This study investigated the occurrence of Mollicutes and elucidated their risk factors in dairy herds from Southeast Brazil. For this, milk samples from 387 lactation cows from Minas Gerais, Rio de Janeiro and São Paulo States were subjected to the polymerase chain reaction (PCR) to detect Mollicutes. Species of Mycoplasma were investigated in Mollicutes positive samples by PCR, including Mycoplasma bovis, M. alkalescens, M. bovigenitalium, M. bovirhinis, M. arginini and A. laidlawii. An epidemiological questionnaire was applied to collect data on possible risk factors, which were assessed using Pearson’s Chi-square test followed by odds ratio (P≤0.05). Mollicutes were reported in 21% (4/19) of the herds and 4% (16/387) of the animals, while 1% (5/387) were positive for M. bovis and 3% (11/387) for M. arginini. All samples were negative to the other agents. Herds with more than 150 animals [OR=3.51 (95% CI 1.11-11.08)], manual milking [OR=9.97 (95% CI 2.80-35.49)] and not-milking animals with mastitis last [OR=6.54 (95% CI 1.92-22.29)] were risk factors. The presence of these conditions may favor intramammary infection by Mollicutes in dairy herds from Southeast Brazil. This is the first report of M. bovis in Rio de Janeiro and M. arginini in the studied states.

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Identification of subclinical mastitis caused by Mycoplasma spp. from screenings of bulk tanks

Cited by 3 publications

References 14 publications

Expressions of IL4, IL10, and IFN"&#".ord($0).";""&#".ord($0).";" cytokines genes during bacterial mastitis

Expressions of IL4, IL10, and IFN"&#".ord($0).";""&#".ord($0).";" cytokines genes during bacterial mastitis

The Efficiency of Centrifugation in the Detection of Mollicutes in Bovine Milk

Risk factors associated with intramammary colonization with Mollicutes in dairy cattle from Southeast Brazil

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