Avaliação das técnicas de imunodifusão em gel de ágar, ensaio imunoenzimático indireto e reação em cadeia da polimerase no diagnóstico da brucelose ovina

Teixeira, L.S.A.; Mineiro, Ana; Batista, Joilson Ferreira; Santana, Mercedes Vernetta; Soares, Fernando Antônio Ribeiro; Paula, Ney Rômulo de Oliveira; Lima, Dijaci Santos de; Damasceno, Tuanny Creusa Medeiros; Porfirio, Kessia; Lustosa, M.S.C.

doi:10.1590/1678-4162-9948

Cited by 1 publication

(1 citation statement)

References 11 publications

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“…Bovine brucellosis is a zoonotic disease caused by a gram-negative bacterium called Brucella abortus and presents a worldwide distribution [1,2,3] and generates great economic losses in livestock bovine production [4,5] due to abortions, reduction in calf production, increase in calving intervals from 11.5 to 20 months, reduction in milk and meat production by 25% infertility and sterility in animals [6,7]. The presence of this agent in the cattle herd has economic implications as barriers to importing markets and losses such as loss of milk production and carcass condemnations of slaughtered cattle [8,9].…”

Section: Introductionmentioning

confidence: 99%

Sensitivity of acidified tamponed antigens in quality control test validation methodologies

Dias

Galvão

Faria

et al. 2022

Preprint

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Brucellosis is responsible for great economic losses and because it is a zoonotic, chronic, infectious, contagious disease, there is a great interest in its eradication in the cattle rearing in Brazil. One of the most effective measures to combat it is the vaccination of the animals and the diagnosis of positive animals, which contributes to the sanitation of the rearing. For this, it is necessary to use a practical, accessible, rapid and sensitive diagnostic methodology. The objective of this study was to evaluate the uncertainty of the test of relative activity (sensitivity) for acidified tamponed antigen (ATA). This antigen is used for the diagnosis of Brucellosis caused by smooth strains of Brucella ssp. Thus, we verified the efficacy of some parameters such as selectivity, precision (limit of detection and intermediate accuracy) and reproducibility. In the analyzes performed in the tests, the performances of standard antigens were considered. It was verified that the acidified tamponed antigen quality control method showed adequate selectivity, limit of detection, intermediate accuracy, repeatability and reproducibility in the verification of sensitivity tests for brucellosis antigens in the LFDA-MG. No difference was verified in the results of the various replicates. The ATA test, using a stable and sensitive antigen that shows high agreement with the confirmatory test, has a high potential to contribute to the identification of positive animals and sanitation of the herd, as well as to reduce the frequency of brucellosis and its subsequent eradication of the bovine herd.

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Section: Introductionmentioning

confidence: 99%

Sensitivity of acidified tamponed antigens in quality control test validation methodologies

Dias

Galvão

Faria

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

Avaliação das técnicas de imunodifusão em gel de ágar, ensaio imunoenzimático indireto e reação em cadeia da polimerase no diagnóstico da brucelose ovina

Abstract: Avaliação das técnicas de imunodifusão em gel de ágar, ensaio imunoenzimático indireto e reação em cadeia da polimerase no diagnóstico da brucelose ovina

Cited by 1 publication

References 11 publications

Sensitivity of acidified tamponed antigens in quality control test validation methodologies

Sensitivity of acidified tamponed antigens in quality control test validation methodologies

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