Comparative study of direct and indirect immunofluorescence for diagnosis of canine pemphigus foliaceus

Severo, Julia So; Aoki, Valéria; Santana, Aline Elisa; Mantovani, Matheus Matioli; Michalany, Nílceo Schwery; Larsson, Carlos Eduardo

doi:10.1590/1678-4162-9917

Cited by 3 publications

(9 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thirty‐four manuscripts were classified as technique papers, encompassing 5 organ systems: rheumatic (n = 10), 125 , 126 , 127 , 128 , 129 , 130 , 131 , 132 , 133 , 134 hematologic (n = 9), 135 , 136 , 137 , 138 , 139 , 140 , 141 , 142 , 143 dermatologic (n = 8), 144 , 145 , 146 , 147 , 148 , 149 , 150 , 151 endocrine (n = 5), 152 , 153 , 154 , 155 , 156 and gastrointestinal (n = 2; Tables S4 and S6 ). 157 , 158 Each comparison between techniques in a single study occupies a single row in Table S4 .…”

Section: Resultsmentioning

confidence: 99%

“…Eight dermatologic studies were included in our analysis. Six related to pemphigus or pemphigus complex, 144 , 145 , 146 , 147 , 149 , 151 and 2 evaluated subepidermal blistering diseases including EBA. 148 , 150 Consistent with the biomarker studies, specific antigens were mostly unidentified.…”

Section: Resultsmentioning

confidence: 99%

“… 148 , 150 Consistent with the biomarker studies, specific antigens were mostly unidentified. 146 , 147 , 149 Autoantibodies were defined by the morphological features of the skin that they targeted, such as epidermal stratum spinosum, 151 and intercellular or basement membrane. 144 , 145 However 1 study identified anticollagen IV autoantibodies.…”

Section: Resultsmentioning

confidence: 99%

“… 148 All pemphigus complex studies either made comparisons between IHC and IFA, 144 , 145 , 151 or compared modifications to IFAs. 146 , 147 , 149 Most comparisons between IFA and IHC showed correlation between the test results. 144 , 151 However, 1 study showed that immunobridge peroxidase IHC was slightly more sensitive at detecting autoantibodies in pemphigus foliaceus but not in pemphigus vulgaris.…”

Section: Resultsmentioning

confidence: 99%

“…Indirect IFA had higher correlation with histopathology results than did direct IFA. 149 The 2 studies on subepidermal blistering disease both studied EBA, 148,150 1 of which also included cases of mucous membrane pemphigoid and bullous pemphigoid. 150 In the study on EBA only, immunoperoxidase IHC collagen IV staining on formalin-fixed, paraffin-embedded skin substrate was introduced to augment standard histopathologic analysis owing to its low cost and relatively high sensitivity.…”

Section: Dermatologic (N = 8)mentioning

confidence: 99%

See 4 more Smart Citations

A scoping review of autoantibodies as biomarkers for canine autoimmune disease

Treeful

Coffey

Friedenberg

2022

Veterinary Internal Medicne

View full text Add to dashboard Cite

Background Autoantibody biomarkers are valuable tools used to diagnose and manage autoimmune diseases in dogs. However, prior publications have raised concerns over a lack of standardization and sufficient validation for the use of biomarkers in veterinary medicine. Objectives Systematically compile primary research on autoantibody biomarkers for autoimmune disease in dogs, summarize their methodological features, and evaluate their quality; synthesize data supporting their use into a resource for veterinarians and researchers. Animals Not used. Methods Five indices were searched to identify studies for evaluation: PubMed, CAB Abstracts, Web of Science, Agricola, and SCOPUS. Two independent reviewers (AET and ELC) screened titles and abstracts for exclusion criteria followed by full‐text review of remaining articles. Relevant studies were classified based on study objectives (biomarker, epitope, technique). Data on study characteristics and outcomes were synthesized in independent data tables for each classification. Results Ninety‐two studies qualified for final analysis (n = 49 biomarker, n = 9 epitope, and n = 34 technique studies). A high degree of heterogeneity in study characteristics and outcomes reporting was observed. Opportunities to strengthen future studies could include: (1) routine use of negative controls, (2) power analyses to inform sample sizes, (3) statistical analyses when appropriate, and (4) multiple detection techniques to confirm results. Conclusions These findings provide a resource that will allow veterinary clinicians to efficiently evaluate the evidence supporting the use of autoantibody biomarkers, along with the varied methodological approaches used in their development.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Dermatologic (N = 8)mentioning

confidence: 99%

See 3 more Smart Citations

A scoping review of autoantibodies as biomarkers for canine autoimmune disease

Treeful

Coffey

Friedenberg

2022

Veterinary Internal Medicne

View full text Add to dashboard Cite

show abstract

New Diagnostic Tools and Tests for Dermatology

White¹

2021

Diagnostics and Therapy in Veterinary Dermatology

View full text Add to dashboard Cite

Pênfigo Dermatofítico - Relato de Caso

Costa¹,

Carvalho²

2022

Rev.S.Dig.

View full text Add to dashboard Cite

Introdução: O Pênfigo dermatofítico é uma doença cutânea extremamente rara, com baixas casuísticas no Brasil, essa enfermidade apresenta-se da mesma forma que o pênfigo superficial, acometendo a barreira mucocutânea de cães e gatos. Objetivo: relatar um caso de pênfigo dermatofítico em um canino, fêmea, SRD, de 4 anos, de 26 Kg, o qual foi atendido no Hospital Veterinário Escola do UNIFAA. Relato de caso: O animal apresentava inicialmente alopecia, eritema, erosão, crostas melicéricas, descamação e colarinho epidérmico. Foi realizada uma cultura fúngica e uma biópsia, onde foi possível observar a presença do fungo Trichophyton spp. No exame histopatológico o resultado compatível com pênfigo foliáceo, com isso foi possível diagnosticar que o animal apresentava um quadro incomum de dermatofitose e pênfigo ocorrendo de forma simultânea, não sabendo exatamente qual enfermidade causou a outra. Foi instituído o tratamento com mupriocina tópica e diprogenta® creme nas lesões, deflazacort na dose de 0,15 mg/Kg SID até a remissão das lesões no tratamento reativo, no tratamento pró-ativo duas vezes por semana e depois substituindo por azatioprina 2,0 mg/kg SID 30 dias, depois reduzindo para 1,0 mg/kg SID e ração light. Resultados: O animal apresentou melhora total, sem apresentar novamente os sinais clínicos e com o exame de cultura fúngica negativa, porém na ausência do imunossupressor as lesões alopecias ulcero-crostosas retornavam concluindo diagnóstico de pênfigo superficial. Conclusão: Por ser uma doença crônica e autoimune, o paciente precisara realizar o controle dela pelo resto de sua vida, com o uso de medicação diária a base de imunossupressores.

show abstract

Comparative study of direct and indirect immunofluorescence for diagnosis of canine pemphigus foliaceus

Cited by 3 publications

References 19 publications

A scoping review of autoantibodies as biomarkers for canine autoimmune disease

A scoping review of autoantibodies as biomarkers for canine autoimmune disease

New Diagnostic Tools and Tests for Dermatology

Pênfigo Dermatofítico - Relato de Caso

Contact Info

Product

Resources

About