Distribution of the stx1 and stx2 genes in Escherichia coli isolated from milk cattle according to season, age, and production scale in southwestern region of Goiás, Brazil

Ferreira, Marcos Roberto Alves; Stella, Ariel Eurides; Filho, Edismauro Garcia Freitas; Silva, T.S.; Nascimento, Karla Alvarenga; Pinto, Jefferson Fernando Naves; Dias, Moacir de Souza; Moreira, Cecília Nunes

doi:10.1590/1678-4162-9861

Cited by 10 publications

(8 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This farm had a high occurrence of isolates containing the gene stx2 in milk and feces samples, and one isolate in water samples. In this property, there might have been cross-contamination among animal feces, milking parlor water, and raw milk due to high environmental contamination, demonstrating the need for hygienic practices (36) .…”

Section: Resultsmentioning

confidence: 99%

DIARRHEAGENIC Escherichia coli IN RAW MILK, WATER, AND CATTLE FECES IN NON-TECHNIFIED DAIRY FARMS

Ribeiro¹,

Barbosa

Pinto

et al. 2019

Ciênc. anim. bras.

View full text Add to dashboard Cite

This study focused on detecting diarrheagenic Escherichia coli, enteropathogenic E. coli (EPEC), Shiga-toxin-producing E. coli (STEC), enterohemorrhagic E. coli (EHEC or STEC:EPEC), enterotoxigenic E. coli (ETEC), and enteroaggregative E. coli (EAEC) in raw milk, water, and cattle feces sampled from non-technified dairy farms located in the northeastern São Paulo State, Brazil. Thirty-six water samples were collected at different points, namely, water wells (8 samples), water intended for human consumption (8 samples), water from milking parlor (8 samples), and water intended for animal consumption (7 samples), headwaters (1 sample), rivers (3 samples), and reservoirs (1 sample). Three raw milk samples were taken directly from bulk tanks in each farm, totalizing 24 samples. Feces samples were collected using rectal swabs from 160 bovines (20 animals per farm). E. coli was detected in 128 feces samples (80%), 16 raw milk samples (66.67%), and 20 water samples (55.56%). STEC (26 samples, 16.25%), EPEC (10 samples, 6.25%), STEC: EPEC (5 samples, 3.13%), and STEC: ETEC (1 sample, 0.63%) were the most prevalent strains detected in samples from cattle feces. EPEC, STEC, and STEC: EPEC strains were detected in 4.17% (1 sample), 16.67% (4 samples), and 4.17% (1 sample) of raw milk samples, respectively. STEC strains were detected in water used in the milking parlor, while no EAEC strain was detected. As a conclusion, cattle feces are important contamination sources of pathogenic E. coli in non-technified dairy farms and, consequently, cross-contamination among feces, water, and/or raw milk can occur. The use of quality water and hygienic practices during milking are recommended to avoid contamination since pathogens can be transmitted to humans via raw milk or raw milk cheese ingestion.

show abstract

Section: Resultsmentioning

confidence: 99%

DIARRHEAGENIC Escherichia coli IN RAW MILK, WATER, AND CATTLE FECES IN NON-TECHNIFIED DAIRY FARMS

Ribeiro¹,

Barbosa

Pinto

et al. 2019

Ciênc. anim. bras.

View full text Add to dashboard Cite

show abstract

“…Cattle are the major STEC reservoir, and beef has been essentially identified as the main vehicle associated with the transmission of this group of microorganisms [52]. The mean stx prevalence in cattle estimated by the QMRA (25.1%, 6.2-64.4%, 95.0% CI) was in the range of that reported in Brazil, USA, Italy and Spain (21.3-36.2%) [87][88][89][90][91]. The STEC prevalence reported in studies conducted in Argentina is also within the same range (11.8-38.9%) [38,39,52,53].…”

Section: Cattle Primary Productionmentioning

confidence: 87%

Quantitative risk assessment of haemolytic uremic syndrome associated with beef consumption in Argentina

et al. 2020

View full text Add to dashboard Cite

We developed a quantitative microbiological risk assessment (QMRA) of haemolytic uremic syndrome (HUS) associated with Shiga toxin-producing Escherichia coli (STEC)-contaminated beef (intact beef cuts, ground beef and commercial hamburgers) in children under 15 years of age from Argentina. The QMRA was used to characterize STEC prevalence and concentration levels in each product through the Argentinean beef supply chain, including cattle primary production, cattle transport, processing and storage in the abattoir, retail and home preparation, and consumption. Median HUS probability from beef cut, ground beef and commercial hamburger consumption was <10−15, 5.4x10-8 and 3.5x10-8, respectively. The expected average annual number of HUS cases was 0, 28 and 4, respectively. Risk of infection and HUS probability were sensitive to the type of abattoir, the application or not of Hazard Analysis and Critical Control Points (HACCP) for STEC (HACCP-STEC), stx prevalence in carcasses and trimmings, storage conditions from the abattoir to retailers and home, the joint consumption of salads and beef products, and cooking preference. The QMRA results showed that the probability of HUS was higher if beef cuts (1.7x) and ground beef (1.2x) were from carcasses provided by abattoirs not applying HACCP-STEC. Thus, the use of a single sanitary standard that included the application of HACCP-STEC in all Argentinean abattoirs would greatly reduce HUS incidence. The average number of annual HUS cases estimated by the QMRA (n = 32) would explain about 10.0% of cases in children under 15 years per year in Argentina. Since other routes of contamination can be involved, including those not related to food, further research on the beef production chain, other food chains, person-to-person transmission and outbreak studies should be conducted to reduce the impact of HUS on the child population of Argentina.

show abstract

“…Pervez et al, [32] reported that 10% of the isolates of calves were positive for VT1 gene while eaeA not detected. The VT1 gene was detected more frequently in calves than in adult animals [40].…”

Section: Discussionmentioning

confidence: 95%

Untitled

2019

VRV

View full text Add to dashboard Cite

Distribution of the stx1 and stx2 genes in Escherichia coli isolated from milk cattle according to season, age, and production scale in southwestern region of Goiás, Brazil

Cited by 10 publications

References 36 publications

DIARRHEAGENIC Escherichia coli IN RAW MILK, WATER, AND CATTLE FECES IN NON-TECHNIFIED DAIRY FARMS

DIARRHEAGENIC Escherichia coli IN RAW MILK, WATER, AND CATTLE FECES IN NON-TECHNIFIED DAIRY FARMS

Quantitative risk assessment of haemolytic uremic syndrome associated with beef consumption in Argentina

Untitled

Contact Info

Product

Resources

About