Resposta imunológica a antígenos de Hysterothylacium deardorffoverstreetorum de peixes teleósteos

Abstract

RESUMOAnisaquidose é uma doença provocada por parasitos da família Anisakidae e se caracteriza por manifestações gastrointestinais e alérgicas. O Anisakis simplex é o parasito mais patogênico ao homem e altamente alergênico. Porém, outros anisaquídeos também são danosos aos humanos, mas é desconhecida a imunogenicidade dessas larvas. O objetivo deste trabalho foi avaliar o potencial imunogênico do parasito Hysterothylacium deardorffoverestreetorum (HD) em modelo murino. Camundongos da linhagem BALB/c foram divididos em três grupos experimentais e receberam as preparações antigênicas obtidas de larvas de HD: extrato bruto de larvas (EBH), extrato secretado/ excretado de larvas (ESH) e extrato bruto de larvas após excreção/secreção (EEH). Amostras séricas foram obtidas em diferentes dias após imunização para determinação dos níveis de anticorpos específicos pelo ensaio imunoenzimático (ELISA). Os resultados demonstram aumento na produção de imunoglobulina (Ig) G após a segunda imunização, com aumento progressivo após a terceira imunização. Já em relação à IgE, a reatividade foi mais tardia, demonstrando aumento progressivo após a terceira imunização. Foi avaliada a imunidade celular por meio da intradermorreação, como resultado estatisticamente significativo em relação ao controle utilizado. Este experimento é a primeira descrição da potencialidade patogênica desse parasito em mamíferos e representa um avanço no diagnóstico da anisaquidose humana.Palavras-chave: anisaquidose, Hysterothylacium, infecção experimental, modelo murino ABSTRACT Anisaquidosis is a disease caused by parasites of Anisakidae family and is characterized by gastrointestinal and allergic reactions. The Anisakis simplex is a more pathogenic Anisakidae to humans and is highly allergenic. However, other species of this family also have characteristics that are harmful to humans, but little is known about the immunogenicity this parasites. The objective of this study was to experimentally assess the immunogenic potential of the parasite Hysterothylacium deardorffoverestreetorum (H.D) in mice. Mice of inbred BALB/c strain were divided into three groups and received three immunizations of the following antigenic preparations obtained from L3 larvae H.D: Crude larval extract of H.D (CEH) Extract secreted / excreted larvae H.D. (ESH) and crude extract of larvae after excretion / secretion (EEH). Serum samples were obtained on different days after immunization to determine the levels of circulating specific antibodies by enzyme-linked immunosorbent assay (ELISA). The results show increased production of immunoglobulin (Ig

Cestode and nematode larvae of hygienic-sanitary importance parasitizing Percophis brasiliensis (Actinopterygii) collected from fish markets of the municipality of Niterói, RJ, Brazil

“…Larvae of the genus Hysterothylacium should be given consideration as they have been reported to cause gastrointestinal discomfort in humans (Yagi et al, 1996). In addition, the allergenic potential of H. deardorffoverestreetorum and C. multipapillatum, collected from other Brazilian marine fish hosts, was recently demonstrated using a murine model (Ribeiro et al, 2017;Fontenelle et al, 2018). Preventive measures can reduce risks to consumer health, such as the evisceration of fish immediately after capture, which reduces the risk of migration by anisakid and raphidascaridid larvae to host musculature, as suggested by Knoff et al (2007).…”

“…The first clinical case of anisakidosis in Brazil reported the presence of an Anisakis-like larva in the stomach of an adult man (Cruz et al, 2010). Studies using a murine model have reported the allergenic potential of H. deardorffoverestreetorum Knoff, Felizardo, Iñiguez, Maldonado Jr, Torres, Pinto & Gomes, 2012and C. multipapillatum (Drasche, 1882) Baylis, 1920(Ribeiro et al, 2017Fontenelle et al, 2018).…”

Cestode and nematode larvae of hygienic-sanitary importance parasitizing Balistes capriscus Gmelin, 1789, collected from fish markets of the state of Rio de Janeiro, Brazil

“…However, even the larvae of Hysterothylacium should be given consideration as they have been reported to cause gastrointestinal discomfort in humans (Yagi et al, 1996). The allergenic potential of H. deardorffoverestreetorum collected from other Brazilian marine fish hosts, was recently demonstrated using a murine model (Ribeiro et al, 2017). Preventive measures can reduce risks to consumer health, such as the evisceration of fish immediately after capture to reduce the risk of migration by raphidascaridid larvae to host musculature, as suggested by Diniz et al (2021).…”

“…An autochthonous case causing human anisakidosis in Brazil was reported by Cruz et al (2010). The allergenic potentials of H. deardorffoverestreetorum and C. multipapillatum (Drasche, 1882) Baylis, 1920 have been showed using a murine model (Ribeiro et al, 2017;Fontenelle et al, 2018).…”

Sensitization with Eustrongylides sp. (Nematoda: Dioctophymatidae) antigens induce production of specific IgG and IgE in murine model

“…are known to be capable of sensitizing the immune system, resulting in severe allergic and gastrointestinal reactions (Hochberg & Hamer, 2010;Cho et al, 2014;Morozińska-Gogol, 2019). Recent studies have demonstrated the potential of immunological sensitization in murine model by Contracaecum multipapillatum (Fontenelle et al, 2018) and Hysterothylacium deardorffoverstreetorum (Ribeiro et al, 2017) larvae. Skin sensitization by food allergens have been reported (Perkin et al, 2020), and antigens applied by the epicutaneous route have been used as potent inducers of food allergies (Wang et al, 1996;Hsieh et al, 2003;Dunkin et al, 2011), suggesting that epicutaneous exposure plays an important role in the cause of atopic allergic dermatitis.…”