Confirmation and Sequence analysis of N gene of PPRV in South Xinjiang, China

Liu, Yonghong; Yang, Bo; Li, Xianqiang; Wang, Xuefeng; Zhang, Luyao; Li, Fēi; Zhao, Li

doi:10.1590/1678-4162-9277

Cited by 3 publications

(2 citation statements)

References 17 publications

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“…Sequence comparison showed a high level of homology (99.18%) of the circulating viruses suggesting that these viruses do not undergo rapid genetic changes in the N gene. This finding is in agreement with other studies (Güler, Şevik, & Hasöksüz, 2014;Liu et al, 2017). The phylogenetic tree constructed from N gene sequences available in GenBank and the partial sequence of the PPRV isolates from Palestine (Figure 2) clearly shows that the circulating PPRV in Palestine belongs to lineage IV which is most abundant PPRV lineage circulating in the Middle East, Asia and recently in Africa (Abd El-Rahim, Sharawi, Barakat, & El-Nahas, 2010;Fakri et al, 2016;Wang et al, 2009).…”

Section: Gross and Histopathologysupporting

confidence: 91%

A molecular and pathological study of peste des petits ruminants virus (PPRV) from field outbreaks in Palestine, 2017‐2019

Fayyad

Alzuheir

Jalboush

2020

Transbound Emerg Dis

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Peste des petits ruminants (PPR) is a severe, highly contagious and fatal viral disease of small ruminants causing high economic losses. Therefore, the disease was targeted for eradication by 2030. The aim of this study was to investigate for the first time the molecular and pathological characterization of the circulating PPR virus (PPRV) in sheep and goat in Palestine. Samples were collected from suspected necropsy cases of sheep and goats during recent outbreaks in two provinces in Palestine between 2017 and 2019. In this study, severe PPR outbreaks occurred in sheep and goats causing typical lesions which include erosive and ulcerative stomatitis, bronchointerstitial pneumonia and severe enteritis. For the molecular investigation of PPRV, suspected animals were examined for the presence of PPRV by RT‐PCR. PPRV genome was detected in all samples. Subsequently, two samples were used for N gene sequencing and phylogenetic analysis of PPRV isolates. The nucleotide sequence and phylogenetic analysis indicated that the Palestinian PPRV isolates were genetically clustered within the lineage IV isolates of the virus among populations of sheep and goats which most prevalent in Asia, the Middle East and recently Africa. Further analysis showed that the Palestinian isolates were closely related to those described in Turkey and Iraq, suggesting a common origin of PPRV isolates in the region. This information is critical to understand the molecular epidemiology of this disease in the region and helps to develop appropriate control measures for eradication of this disease.

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Section: Gross and Histopathologysupporting

confidence: 91%

A molecular and pathological study of peste des petits ruminants virus (PPRV) from field outbreaks in Palestine, 2017‐2019

Fayyad

Alzuheir

Jalboush

2020

Transbound Emerg Dis

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“…However, eight and two strains detected in the present study were classified to subtype lineage IV-I and IV-II, respectively. Lineage IV was associated with previous outbreaks reported from neighboring Asian countries [ 22 , 33 , 34 ].…”

Section: Discussionmentioning

confidence: 85%

Pheno- and genotypic characterization and identification of novel subtypes of Peste des Petits Ruminants virus in domestic and captive wild goats in Northern Iraq

Khoran¹,

Candlan²,

Hassan

et al. 2021

BMC Microbiol

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Background Peste des Petits Ruminants (PPR) is an acute or peracute contagious transboundary viral disease that mainly affects caprine and ovine and causes significant economic impact in developing countries. After two PPR virus outbreaks in 2011 and 2014, an investigation, from August 2015 to September 2016, was carried out in Northern Iraq when an increased morbidity and mortality rates were reported in the domestic and captive wild goats. In the present study, ten domestic goat farms and seven captive wild goat herds located in seven geographical areas of Northern Iraq were clinically, pathologically, serologically and genotypically characterized to determine the prevalence and potential cause of PPR virus outbreak. Results The outbreak occurred with rate of morbidity (26.1%) and mortality (11.1%) in domestic goat farms as compared to captive wild goat herds where relatively high mortality (42.9%) and low morbidity (10.9%) rates were recorded. Based on the clinical symptoms (mucopurulent nasal discharges, ulceration and erosion of oral mucosa, profuse watery diarrhea) and necropsy (hemorrhage and congestion on mucous membranes of the colon and rectum with zebra stripes lesions) results, overall, the serological test findings revealed a high frequency (47.9%) of positive samples for anti-PPRV nucleoprotein antibodies. Furthermore, the nucleoprotein (N) gene was detected in 63.2 and 89.1% of samples using conventional and reverse transcription real-time quantitative PCR assays. A phylogenetic analysis of N gene amino acid sequences clustered with the reference strain revealed lineage IV similar to the strains isolated in 2011 and 2014, respectively. However, two sub-types of lineage IV (I and II), significantly distinct from the previous strains, were also observed. Conclusion The phylogenetic analysis suggests that movements of goats are possible cause and one of the important factors responsible for the spread of virus across the region. The study results would help in improving farm management practices by establishing a PPR virus eradication program using regular monitoring and vaccination program to control and mitigate the risk of re-emergence of PPR virus infection in domestic and captive wild goats in Iraq.

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Neuropathology mediated through caspase dependent extrinsic pathway in goat kids naturally infected with PPRV

Sahoo

Dinesh

Thakor

et al. 2020

Microbial Pathogenesis

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Confirmation and Sequence analysis of N gene of PPRV in South Xinjiang, China

Cited by 3 publications

References 17 publications

A molecular and pathological study of peste des petits ruminants virus (PPRV) from field outbreaks in Palestine, 2017‐2019

A molecular and pathological study of peste des petits ruminants virus (PPRV) from field outbreaks in Palestine, 2017‐2019

Pheno- and genotypic characterization and identification of novel subtypes of Peste des Petits Ruminants virus in domestic and captive wild goats in Northern Iraq

Neuropathology mediated through caspase dependent extrinsic pathway in goat kids naturally infected with PPRV

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