Involvement of mast cells, CD68+ and VEGF+ expressions in response to Himatanthus drasticus commercial latex in mice wound healing model

Abstract

This study aimed to evaluate Himatanthus drasticus latex in a mice wound healing experimental model. Animals were divided into four groups (n=7) according to the treatments: GI -saline 0.9% (control), GIImineral oil (vehicle), GIII -H. drasticus commercial latex (HdCL) and GIV -H. drasticus mixed isolated fraction (MIF, 1 mg/mL). The treatments were applied topically once daily, 50 µL for 14 consecutive days. Macroscopic lesions were evaluated, considering parameters such as swelling, redness, granulation tissue and reepithelialization. VEGF+, CD68+ expressions and mast cells (Toluidin blue stain) were evaluated. HdCL induced higher contraction and exuberant granulation tissue (P > 0.05). HdCL showed a mild inflammatory process while MIF induced intense infiltrate inflammatory predominantly by lymphocytes, vascular congestion, bleeding and did not presented full reepithelialization. Reorganization of collagen fibers (red picrosirius stain) was observed. CD68+ expression and mast cells were presented as moderate, intense and mild in GI, GIII and GIV, respectively. Neovascularization occurred in all groups, while VEGF+ expression was intense in MIF in relation to HdCL. We concluded that HdCL presents wound healing potential, through modulation of mast cells, CD68+ and VEGF+ expressions that can be associated to triterpenes presence according MIF isolated from HdCL.Keywords: latex, Apocinaceae, wound healing, angiogenesis, inflammatory infiltrate RESUMO Objetivou-se avaliar o látex de Himatanthus drasticus em feridas induzidas experimentalmente em camundongos. Os animais foram divididos em quatro grupos (n=7): GI -salina 0,9% (controle), GIIóleo mineral (veículo), GIII -látex comercial de H. drasticus (HdCL) e GIV -fração isolada mista de H. drasticus (MIF, 1mg/mL). Os tratamentos foram aplicados topicamente uma vez ao dia (50µL), durante 14 dias consecutivos. Lesões macroscópicas, as expressões de VEGF+, CD68+ e a participação dos mastócitos (coloração azul de toluidina) foram avaliadas. HdCL induziu maior contração e tecido de granulação exuberante (P >0,05). HdCL induziu leve processo inflamatório enquanto MIF promoveu intenso infiltrado inflamatório predominantemente linfocítico, congestão vascular, hemorragia e reepitelização parcial. Observou-se reorganização das fibras colágenas (coloração picrosírius

Phytochemical prospection and larvicidal bioactivity of the janaguba (Himatanthus drasticus) Mart. Plumel (Apocynaceae) latex against Aedes aegypti L. (Diptera: Culicidae)

“…Flavonoids were also found by Santos et al (2017) in photochemical screening studies and evaluation of the healing activity of ethyl acetate extract of H. drasticus latex on mice. In addition, Santos et al (2018) evaluated the antitumor activity of ethyl acetate extract of H. drasticus latex on mice and in photochemical screening, these authors also verified the presence of flavonoids in the tested extract.…”

“…This species is highly sought after by the population due to the popular use of its latex in folk medicine for the treatment of gastritis, hemorrhoids, anemia, inflammation, and even cancer (Morais et al, 2020). Scientifically it is proven that the janaguba latex has antibacterial (Nascimento et al, 2018), gastroprotective (Colares et al, 2008;Leite et al, 2009), anti-inflammatory (Almeida et al, 2019), healing (Santos et al, 2017), and antitumor activity (Santos et al, 2018).…”

Revisão da literatura científica de Himatanthus drasticus (Mart.) Plumel

“…O látex de H. drasticus apresenta ainda potencial no processo de cicatrização de feridas em camundongos, corroborando com sua indicação na medicina popular tradicional (Santos et al, 2017). Esse efeito ocorre pela modulação positiva da atividade de mastócitos e das expressões de CD68+ e VEGF+.…”