Aspectos citoquímicos e morfológicos de elementos sanguíneos das serpentes dos gêneros Bothrops e Crotalus mantidas em cativeiro no serpentário do Instituto Vital Brasil

Abstract

Aspectos citoquímicos e morfológicos de elementos sanguíneos das serpentes dos gênerosBothrops e Crotalus mantidas em cativeiro no serpentário do Instituto Vital Brasil

Hematologic parameters of captive Bothrops atrox (Squamata: viperidae)

“…In Brazil, Kindlovits et al (2017) investigated the cytochemical and morphological aspects of blood cells of snakes of the genus Bothrops and Crotalus. Blood was collected from 50 captive animals from the Instituto Vital Brasil, and eight specimens of the species B. atrox showed parasites of the genus Hepatozoon, which did not occur in this study.…”

“…Blood cells found in reptiles include erythrocytes and nucleated thrombocytes, heterophils, eosinophils, basophils, monocytes, lymphocytes, and azurophils (Campbell 2014;Heatley & Russel, 2019). According to Heatley & Russel (2019), eosinophils are rare or absent in most snakes and their presence is controversial, although it has been previously described in recent work such as that of Kindlovits et al (2017) with the genus Bothrops (B. atrox and B. jararacussu) and Corallus hortulanus, and the research of Quadrini et al (2018), with Python bivittaus. Reptile erythrocytes are ellipsoid, permanently nucleated, and larger than those of birds and mammals (Campbell 2014).…”

“…However, specialized veterinarians often encounter difficulties in obtaining laboratory reference values for the prophylactic management of diseases in these reptiles. In the literature, there is little information on the hematological, biochemical and clinical parameters of the species B. atrox, and the characterization of such data is important in the evaluation of the health of the breeding population, diagnosis and therapeutic follow-up (Trujillo et al 2016;Kindlovits et al 2017;Troiano, 2018).…”

Hematologic and biochemical reference intervals and blood cell morphology in South American pit vipers (Bothrops pubescens)

“…19 In our study, eosinophilic-like cells similar to eosinophils in stained blood smears were not observed, as in other hematologic studies for snakes. 12 Previous studies reported eosinophils in some snake species, 16,19,20 although researchers have classified them as differentiated heterophils based on specific stains. 12,21 To confirm WBC types and differentiate eosinophils and heterophils in some snake species, further studies using flow cytometry or cytochemical staining techniques should be considered.…”