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(5 citation statements)
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“…Several previous studies that compared the PCR-PARR technique with conventional immunohistochemistry revealed an agreement of >85% [10,14]. Therefore, the application of the real-time PCR-PARR technique in the veterinary medical routine proves to be promising, helping the veterinarian in the diagnosis and establishment of the prognosis of canine lymphomas, as well as in retrospective studies to confirm the clonality of lymphomas.…”
Section: Discussionmentioning
confidence: 92%
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“…Several previous studies that compared the PCR-PARR technique with conventional immunohistochemistry revealed an agreement of >85% [10,14]. Therefore, the application of the real-time PCR-PARR technique in the veterinary medical routine proves to be promising, helping the veterinarian in the diagnosis and establishment of the prognosis of canine lymphomas, as well as in retrospective studies to confirm the clonality of lymphomas.…”
Section: Discussionmentioning
confidence: 92%
“…It was observed that some samples had a low amount of DNA, which is expected, since the histopathological processing to which the samples are submitted, using xylol and formalin, for example, favors the occurrence of DNA lesions, impairs its quality [12], and interferes with the amount of DNA present, generally lower, with no good amplification in conventional PCR [8,10,15].…”
Section: Discussionmentioning
confidence: 99%
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