Kinematic and spermatic recovery after selection by centrifugation in colloid solutions of ovine cryopreserved semen

Bergstein, T.G.; Bicudo, L. C.; Rodello, Leandro; Weiss, Romildo Romualdo; Bicudo, Sony Dimas

doi:10.1590/1678-4162-8912

Cited by 4 publications

(4 citation statements)

References 26 publications

(17 reference statements)

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“…The method that used 1 ml colloidal silica–silane was the most effective in selecting semen with desirable characteristics, and reduced the incidence of spermatozoa with undesirable characteristics, such as damaged plasma membranes or reacted acrosomes. The method that used 1 ml colloidal silica–silane was the best method identified in this study corroborating the results reported by (Bergstein, Bicudo, Rodello, Weiss, & Bicudo, ) who studied the kinematics of ovine cryopreserved semen selected by colloidal silica–silane.…”

Section: Discussionsupporting

confidence: 90%

Sperm membrane integrity and stability after selection of cryopreserved ovine semen on colloidal solutions

et al. 2017

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The aim of this study was to evaluate the effect of four methods of sperm selection, on the integrity and stability of the plasma membrane, integrity of the acrosomal membrane and spermatic morphology in frozen/thawed ovine semen. Two types of colloidal silica: colloidal silica-silane and colloidal silica-polyvinylpyrrolidone (PVP), and two aliquots: 1 and 4 ml, were used for sperm selection. Probes FITC-PSA and PI were used to measure the integrity of the plasma and acrosomal membranes. Plasma membrane stability was measured, using fluorescent probes M540 and YOPRO1. Effective reduction in the incidence of spermatozoa with acrosomal pathologies was only achieved using 1 ml colloidal silica-silane. All methods were efficient in select viable and unreacted spermatozoa. Only methods using 1 ml of silica were efficient in decrease spermatozoa stained by PI (death). Methods using silica colloidal-silane were more efficient to decrease apoptotic cells after selection when compared to silica colloidal-PVP. In conclusion, sperm selection in colloidal silica-silane and colloidal silica-PVP improved sperm quality when compared to the controls. The method using 1 ml of colloidal silica-silane is the preferred method because its effectiveness and lower cost.

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Section: Discussionsupporting

confidence: 90%

Sperm membrane integrity and stability after selection of cryopreserved ovine semen on colloidal solutions

et al. 2017

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“…The results obtained after centrifugation and different fractions isolation suggest that SLC does not separate the best motility quality subpopulation, whether in C800 or in C300 sperm samples, not being SP2 the most abundant subpopulation in F3, as expected. It was observed that many spermatozoa belonging to SP2 (considered as the best subpopulation) were retained in the second interface; one possible explanation for this observation is that the high number of spermatozoa included in the sample may impede other optimal spermatozoa from swimming to the bottom of the tubes (30) . As previously recommended (31) , SLC it is better to use for processing of large volumes of semen, suggesting that the increment of the sperm concentration was an impediment to the efficient operation of this method.…”

Section: Discussionmentioning

confidence: 99%

Las subpoblaciones de espermatozoides y su calidad en fracciones producidas por la centrifugación de una sola capa en muestras frescas y normospérmicas de esperma de cordero

Marı́n¹,

Arbulu

Maroto-Molina

et al. 2021

Rev. Mex. Cienc. Pecu.

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Single layer centrifugation (SLC) technique has been developed to select the best sperm population in the ejaculate in order to increase the fertilization rates by artificial insemination or in vitro fertilization. Normospermic ram semen samples containing 800 and 3,000 × 106 sperms/ml (C800 and C3000, respectively) were processed by SLC. Three sperm fractions were separated in each sample following silica-coloidal sperm centrifugation and sperm yield, quality and subpopulations were analyzed in each one. In C800 group, the sperm recovery rate did not vary in any studied fraction, but when samples were highly concentrated (C3000) the top fraction (F1) contained significantly higher spermatozoa than bottom fraction (F3). Also, it was observed that F1 in C3000 had got a significantly higher percentage of spermatozoa (53.2 %) than in C800, while the quantity of spermatozoa recovered in fraction 2 was lower (25.2 % vs 45.4 %). Based on the sperm motility parameters, three sperm subpopulations were identified: SP1, low velocity spermatozoa showing no progressive movement (19.1 %); SP2, rapid and progressive spermatozoa (43.7 %); and SP3, rapid spermatozoa but non-linear movement (37.2 %). While SLC has been implemented for sperm separation in suboptimal and/or low concentrated sperm samples, this trial demonstrates that SLC is not efficient to separate different sperm populations in normospermic ram sperm samples containing high concentrations of spermatozoa.

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“…Ovine ejaculate is composed of a heterogeneous population of sperm cells (Bergstein et al, 2016;García-Álvarez et al, 2010). The…”

Section: Discussionmentioning

confidence: 99%

Sperm subpopulations in ejaculated sperm and spermatozoa recovered from ovine epididymides up to 48 h after death

Bergstein-Galan

Weiss

Kozicki

et al. 2017

Animal Reproduction Science

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The objectives of this study were threefold: to identify subpopulations of sperm based on the kinetics of frozen/thawed sheep epididymal spermatozoa or semen collected with an artificial vagina; to evaluate the effects on sperm subpopulations in the thawed samples of post mortem storage at room temperature and the addition of 20% of seminal plasma to the freezing extender and to correlate the percentage of subpopulations with gestation rate following artificial intrauterine insemination. The categorization of the subpopulations was based on sperm kinetic data from Computer Assisted Sperm Analysis (CASA). A hundred ewes were inseminated with thawed spermatozoa and gestation rate was correlated with the proportions of each subpopulation using Pearson correlation matrix and linear regression. Three distinct subpopulations were identified in the thawed samples of either ovine ejaculate collected in artificial vaginas (AV) or ovine spermatozoa retrieved from the cauda epididymis. Subpopulation 1 (SP1) was characterized by spermatozoa with slow and non-linear motion, subpopulation 2 (SP2) was classified as hyperactived spermatozoa and subpopulation 3 (SP3) was composed of spermatozoa with fast, linear motion. The largest subpopulation in all groups was SP1. The semen collected in an artificial vagina had a higher (P<0.05) percentage of SP2 and lower (P<0.05) percentage of SP1 when compared to spermatozoa recovered after death. Increasing time of storage after death had a detrimental effect on sperm samples, increasing (P<0.05) the percentage of SP1 and decreasing (P<0.05) SP2. Length of storage after death was the only variable that influenced, with an inversely proportional relationship, SP3. In samples stored for 48h after death no SP3 spermatozoa were present. The addition of seminal plasma to the cryopreservative decreased (P<0.05) the subpopulation of hyperactived spermatozoa (SP2). We conclude that, after thawing there are three sperm subpopulations in the spermatozoa obtained from the cauda epididymides and the semen collected in AVs and that the relative proportions of these subpopulations varies with the time of storage post mortem and the presence of 20% of seminal plasma in the extender. However, we conclude that these subpopulations do not correlate with fertility after intrauterine artificial insemination.

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Kinematic and spermatic recovery after selection by centrifugation in colloid solutions of ovine cryopreserved semen

Cited by 4 publications

References 26 publications

Sperm membrane integrity and stability after selection of cryopreserved ovine semen on colloidal solutions

Sperm membrane integrity and stability after selection of cryopreserved ovine semen on colloidal solutions

Las subpoblaciones de espermatozoides y su calidad en fracciones producidas por la centrifugación de una sola capa en muestras frescas y normospérmicas de esperma de cordero

Sperm subpopulations in ejaculated sperm and spermatozoa recovered from ovine epididymides up to 48 h after death

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