Establishment of an anesthetic protocol for semen collection by electroejaculation in six-banded armadillos (Euphractus sexcinctus Linnaeus, 1758)

Sousa, Patrícia Cunha; Amorim, Raul; Lima, Gabriela Liberalino; Paiva, Ariana Lopes Corrêia de; Paula, Valéria Veras de; Freitas, Carlos Iberê Alves; Silva, Alexandre Rodrigues

doi:10.1590/1678-4162-8811

Cited by 10 publications

(7 citation statements)

References 20 publications

(8 reference statements)

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“…As the latter was alone at the moment it leaned against the corner and started to vocalize, we assume that the subject was probably manifesting high-stress levels. This has already been reported for individuals of Euphractus sexcinctus (six-banded armadillo) submitted to electroejaculation (EEJ) to obtain semen, after which they showed intense vocalization (Sousa et al, 2016). This vocalization is not explained nor described, but these armadillos are known to produce a low, continuous, purr-like sound while vibrating when handled (Costa, pers.…”

Section: Discussionmentioning

confidence: 88%

“…Members of the Dasypodidae (the only living family of cingulates) are reported to vocalize in different forms and contexts: while foraging (grunting sound), pairing (chuck sound), communicating with mother/young (buzz sound), or being disturbed (scream or growl sound) (Talmage & Buchanan, 1954;Anderson & Jones-Jr., 1984;Nowak, 1999;Feldhamer et al, 2003). However, despite much of these sounds having been reported in previous studies (e.g., Superina, 2008;Smith, 2011;Abba & Superina, 2015;Sousa et al, 2016), very few studies have actually described them. Except for the classical paper of Christensen & Waring (1980) who provided a sound spectrogram to detail the "chuck" sound of Dasypus novemcinctus, and the more recent study of Amaya et al (2019) who presented a detailed description of the weeping vocalization of Chaetophractus vellerosus, studies of this kind are lacking.…”

Section: Introductionmentioning

confidence: 95%

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Acoustic analysis of vocalization and the behavioral response associated to sound production of the nine banded armadillo Dasypus novemcinctus (Mammalia, Cingulata, Dasypodidae) in an agonistic context

et al. 2022

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Although communication capabilities are displayed by many vertebrate groups, some repertoires are poorly known, such as the case of xenarthrans, particularly armadillos, for which vocalization as a source of communicating to others remains poorly understood and relies on punctual reports of sounds. Here we provide the first description of a behavioral response associated with sound emission of two subjects of Dasypus novemcinctus. Both audio and visual registration was performed to subsequent analyses of expressed behaviors and emitted calls, which accounted for 76 vocalizations from a total of eight video recordings randomly collected from 2017 to 2019. Sound is acoustically characterized by both inhale and exhale phases composed of two vocal units, and no harmonic structure was observed. Once the subjects have always produced these vocalizations while cornered and exhibiting defensive behavior against another subject/human disturbance, these vocalizations were termed as distress. Subjects produced a hiss-purr-like sound while trying to avoid contact with another by bowing or lowering their bodies, humping, or even moving elsewhere when sound production ceased. This shows that the sound repertoire of armadillos is still to be unveiled and seems to be much more complex than previously thought.

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Section: Discussionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 95%

Acoustic analysis of vocalization and the behavioral response associated to sound production of the nine banded armadillo Dasypus novemcinctus (Mammalia, Cingulata, Dasypodidae) in an agonistic context

et al. 2022

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“…The lower sperm concentration found in the detomidine group compared to medetomidine resulted in a greater amount of seminal fluid. This can lead to osmotic changes, which negatively affect the sperm membrane and interfere with sperm viability, as reported by (Sousa et al, 2016) in six-banded armadillos. The only parameter that did not show any statistical difference was the semen volume.…”

Section: Discussionmentioning

confidence: 94%

Can detomidine replace medetomidine for pharmacological semen collection in domestic cats?

et al. 2021

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Among the different methods used for semen collection from domestic cats, the pharmacological collection by urethral catheterization becomes disruptive. Medetomidine is the elected α 2 -adrenoceptor agonist for that, but in several countries, it is not commercially available. This study aimed to evaluate the efficacy of detomidine compared to medetomidine in collecting semen by urethral catheterization in domestic cats. Urethral catheterization was performed on 13 mongrel cats using a disposable semi-rigid tomcat urinary catheter. Of the 19 semen collections performed with medetomidine induction, 94.7% were successful, while with detomidine induction, only 56.3% of 16 were successful. The values semen samples variables were as follows for volume -10.56 ± 0.4 vs 8.88 ± 0.5 mL, motility -171.67 ± 0.79 vs 49.77 ± 3.45%, vigor -4.1 ± 0.03 vs 3.10 ± 0.1 and concentration -3.24 ± 0.19 vs 2.15 ± 0.13 ×10 9 sperm/mL respectively for medetomidine and detomidine group. The failure in semen collections with detomidine was mainly due to azoospermic samples, poor urethral relaxation, insufficient volume, or contamination of urine. The sperm concentration was also lower in the detomidine group (P <0.05) when compared to medetomidine. However, when the volume of semen collected was compared, we found no statistical differences. Despite its low performance in collecting semen from cats, detomidine may be an alternative when medetomidine is not accessible.

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“…In this study, general anesthesia provides better welfare to the animal as it is painless and less stressful [46]. Apart from that, the drug also has an alpha-adrenergic effect on the smooth muscle involved with semen release, allowing the flow of semen in the reproductive tract to the penile tip [47]. The findings were similar in the use of anesthesia in other wild mammals, using the xylazine combination under general anesthesia, in which the semen can sometimes be collected readily on the penile tip with the manipulation and positioning of the electroejaculator probe as an effect of immobilization [48].…”

Section: Discussionmentioning

confidence: 99%

The Comparison of Semen Collection in Electroejaculation, Rectal Massage and Combination of Both Methods in the Critically Endangered Malayan Pangolin, Manis javanica

Tarmizi

Chee

Sipangkui

et al. 2020

Animals

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This article describes the semen characteristics from different collection methods between captive and confiscated Malayan pangolins, Manis javanica. Semen was collected from 15 pangolins; two captive and 13 confiscated individuals at the mean weight of 9.36 ± 1.94 kg. The three semen collection methods employed were electroejaculation, rectal massage and a combination of both techniques. The semen characteristics (mean ± standard deviation) of the Malayan pangolin are volume (73.75 ± 144.57 µL), pH (7.63 ± 0.53), spermatozoa concentration (997.19 ± 728.98 × 106 /mL), total motility (59.60% ± 30.00%), progressive motility (48.95% ± 30.93%), mass motility (3.50 ± 1.50) and live spermatozoa (80.25% ± 13.45%). There was no significant difference in semen characteristics between the three collection methods. The percentages of live spermatozoa were significantly different, suggesting better samples from captive compared to confiscated animals. However, there was no significant difference in spermatozoa kinetics between the captive and confiscated samples, suggesting the potential of utilizing confiscated individuals for gamete recovery to conserve the genetic pool of pangolins. All three methods of semen collection were successfully performed in pangolins and should be considered; however, electroejaculation remains the most consistent method of obtaining semen from the species.

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Establishment of an anesthetic protocol for semen collection by electroejaculation in six-banded armadillos (Euphractus sexcinctus Linnaeus, 1758)

Cited by 10 publications

References 20 publications

Acoustic analysis of vocalization and the behavioral response associated to sound production of the nine banded armadillo Dasypus novemcinctus (Mammalia, Cingulata, Dasypodidae) in an agonistic context

Acoustic analysis of vocalization and the behavioral response associated to sound production of the nine banded armadillo Dasypus novemcinctus (Mammalia, Cingulata, Dasypodidae) in an agonistic context

Can detomidine replace medetomidine for pharmacological semen collection in domestic cats?

The Comparison of Semen Collection in Electroejaculation, Rectal Massage and Combination of Both Methods in the Critically Endangered Malayan Pangolin, Manis javanica

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