Expressão de metaloproteinases de matriz e PCNA em úlceras de córnea profundas, induzidas em coelhos, tratadas com plasma rico em plaquetas

Perches, Cintia Sesso; Pellizzon, Cláudia Helena; Ranzani, José Joaquim Titton; Donatti, Camila I.; Padovani, Carlos Roberto; Merlini, Natalie Bertelis; Fonzar, Joice Furtado; Beserra, Hugo Enrique Orsini; Rocha, Noeme Sousa; Brandão, Cláudia Valéria Seullner

doi:10.1590/1678-4162-8142

Cited by 4 publications

(5 citation statements)

References 17 publications

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“…Evaluating vascularization in the GP group, a significant increase was noted between M1 and M3 (P<0.05), which can be due to growth factors in PRP, most notably among them vascular endothelial growth factor (VEGF), the main mediator during the corneal vascularization process. This current result was similar to that described by Perches et al (2015), who reported greater vascularization in animals at the end of 30 days of topical PRP treatment and attributed this finding to the VEGF concentration in PRP.…”

Section: Discussionsupporting

confidence: 92%

“…Another possibility would be that the PDGF-BB is not the main isotope present on the rat cornea, or even the main growth factor functioning in the healing process, considering the presence of PDGF-AA, PDGF-AB, PDGF-BB (Penn et al, 2008), and the fact there are two more new members, PDGF-C and PDGF-D, which have protein structures similar to other isoforms (Betsholtz et al, 2001). Despite the absence of changes detected in the PDGF dosed, we could verify positive clinical results in the repairing process after the PRP treatment, heated or not.…”

Section: Allogenic Platelet-rich…mentioning

confidence: 99%

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Allogenic platelet-rich plasma in induced ulcers in rat’s cornea

Gandolfi

Brandão

Pellizon

et al. 2021

Arq. Bras. Med. Vet. Zootec.

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The objective in this study was to evaluate the clinic effect of applying allogenic platelet-rich plasma (PRP) heated or not, for treating cornea ulcers, including the dosage of PDGF-BB in the cornea. The ulcers were induced, standardizing the left eye from 81 rats (Ratus norvegicus, albinus variety), assigned randomly into three groups (N=27): control group (CG) which did not receive any topic treatment; heated PRP group (GA) and PRP group (GP), which received topical treatment every eight hours for five days. Each group underwent evaluation at 24 hours (M1), three days (M3) and five days (M5). The clinical exam evaluated the opacity, vascularization and corneal repair. The corneal PDGF-BB was dosed through the ELISA method. The corneal opacity was decreased in PRP-treated animals (GA and GP) and corneal repair time reduced when compared to CG at M1 and M5. Furthermore, GP showed greater vascularization at M3 compared to M1. Applied allogenic PRP eye drops, heated or not, speed up corneal healing, and reduce corneal repair time. However, the corneal PDGF concentration was not altered in any of the treatments.

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Section: Discussionsupporting

confidence: 92%

Section: Allogenic Platelet-rich…mentioning

confidence: 99%

Allogenic platelet-rich plasma in induced ulcers in rat’s cornea

Gandolfi

Brandão

Pellizon

et al. 2021

Arq. Bras. Med. Vet. Zootec.

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“…Results from G1 presented remodeling and discrete to moderate acanthosis, besides discrete to mixed inflammatory infiltrate at 37 th POD. This result differed from Perches et al 17,18 , who used PPP and PRP for treating corneal ulcers in rabbits, and observed a larger inflammatory infiltrate in the group treated with PRP, comparing to the control (30 th POD), suggesting a positive answer on the expression of matrix metalloproteins.…”

Section: ■ Discussioncontrasting

confidence: 81%

“…Clinical uses of PRP in tissues other than skin are also being widely studied, as described by Anitua et al 19,20 , who used bone grafts to aid implantation process in dental and ophthalmic treatments, by Perches et al 17 , who treated corneal ulcers in rabbits, as well as studies in more complex areas such as cardiovascular treatments described by Mörschbacher et al 21 , who used PRP associated with stem cells to treat dilated cardiomyopathy in rabbits.…”

Section: ■ Discussionmentioning

confidence: 99%

Effects of homologous and heterologous rich platelets plasma, compared to poor platelets plasma, on cutaneous healing of rabbits

Meira

Braga

Pinheiro³

et al. 2020

Acta Cir. Bras.

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Methods: Twenty-one male adult rabbits were used; two for preparing homologous PRP, with the rest of them separated randomly in three groups, according to the treatment received: PPP-control (n=5), homologous PRP (n=7), heterologous (n=7). Excisional skin wounds were made on the back of the animals, for the application of homologous and heterologous PPP and PRP. At the 14 th post-operative day (POD), the animals were subjected to a new wound, and the treatments were inverted. The wounds were evaluated macroscopically and histologically. Results: A larger percentage of scar retraction was observed on the group treated with heterologous PRP, compared to homologous PRP, at the third POD, an increase of 25.03% (p=0.01). No other statistically significant differences among treatments were observed. Among every group, skin healing was efficient, without local adverse effects. Conclusions: Heterologous PRP contributed with more tissue retraction at the beginning of the wound healing process. After this, there were no differences on the wound healing skin process treated with PRP or PPP. However, our findings suggest the presence of others plasmatic factors, besides platelets, which could also contribute to the wound healing process, and thus, should be further investigated.

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“…To date, the study on the effects of PRP on metalloproteinases is limited and the results are controversial. Perches et al [13], in research regarding corneal ulcers treated with PRP, demonstrated that the use of eye drops of platelet-rich and poor plasma affects the expression of matrix metalloproteinases participating in the corneal repair process.…”

Section: Introductionmentioning

confidence: 99%

Effects of platelet-rich plasma on corneal re-epithelization and metalloproteinase expression in the cornea of sheep with experimentally-induced infectious keratoconjunctivitis

et al. 2023

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Background and Aim: Infectious bovine keratoconjunctivitis is the most crucial ophthalmic disease among ruminants worldwide. Moraxella is the bacteria generally associated with this disease and leads to keratitis, conjunctivitis, corneal ulcers, or blindness. Platelet-rich plasma (PRP) effects in corneal ulcers and different ocular superficial diseases in animals and humans are beneficial and enhance rapid healing and improvement, but the effects in infectious keratoconjunctivitis in ruminants are uncertain. This study aimed to examine the effect of PRP on re-epithelization, corneal tissue, clinical signs, and matrix metalloproteinase (MMP) expression in sheep with infectious keratoconjunctivitis. Materials and Methods: Eighteen sheep were divided into three groups and subjected to a disease-induction experiment. Group 1 (G1) was administered 1.0 mL PRP subconjunctivally, Group 2 (G2) was administered 1.0 mL PRP subconjunctivally and 50 μL gentamicin drops, and the control group (CG) was administered 50 μL saline solution topically every 12 h. Clinical ophthalmologic examination, fluorescein staining, and photography were carried out. Ulcerated areas were measured employing J-Image software. Five and eleven days following the procedure, half of the animals from each group were euthanized, and their corneas were evaluated by histopathology and zymography. Results: Control Group and G2 epithelialized more rapidly. The CG exhibited fewer clinical signs of ocular disease. In histopathological analysis, in G2, alterations were observed only in the epithelium. The CG and G1 exhibited alterations in the epithelium, stroma, and Descemet’s membrane. In zymography, a decline in MMP-2 expression in the animals treated with PRP was detected. Matrix metalloproteinase-9 was significantly expressed in the animals treated with PRP monotherapy, whereas PRP + gentamicin and CG caused a decrease. Conclusion: Platelet-rich plasma alone did not demonstrate any beneficial effect on re-epithelialization, a decline in clinical signs, tissue alterations, and expression of metalloproteinases. Platelet-rich plasma combined with gentamicin was capable of suppressing MMPs, primarily MMP-9, but do not display positive effects in re-epithelization, reduction of clinical signs, or tissue effects. These outcomes are similar to those discovered in untreated animals, so the use of PRP in patients with infectious keratoconjunctivitis does not offer greater benefits in sheep. Additional research is required to validate the results of PRP use in natural disease presentation. Keywords: corneal ulcers, experimental model, matrix metalloproteinase, Moraxella spp., pinkeye, platelet-rich plasma.

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Expressão de metaloproteinases de matriz e PCNA em úlceras de córnea profundas, induzidas em coelhos, tratadas com plasma rico em plaquetas

Cited by 4 publications

References 17 publications

Allogenic platelet-rich plasma in induced ulcers in rat’s cornea

Allogenic platelet-rich plasma in induced ulcers in rat’s cornea

Effects of homologous and heterologous rich platelets plasma, compared to poor platelets plasma, on cutaneous healing of rabbits

Effects of platelet-rich plasma on corneal re-epithelization and metalloproteinase expression in the cornea of sheep with experimentally-induced infectious keratoconjunctivitis

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