Detecção de Theileria equi e Babesia caballi e anticorpos anti-Ehrlichia spp. em equídeos do Pantanal Mato-Grossense, Brasil

Abstract

RESUMOO presente estudo avaliou equídeos de 19 fazendas da região do Pantanal Mato-Grossense, sendo 121 equídeos testados pela reação em cadeia pela polimerase (PCR), para detectar fragmentos dos genes dos seguintes gêneros: Babesia, Theileria, Anaplasma, Ehrlichia, e Neorickettsia, e pela reação de imunofluorescência indireta (RIFI), para detectar anticorpos anti-Ehrlichia spp. (Wise et al., 2013). A piroplasmose se manifesta clinicamente desde uma forma aguda, em que os animais apresentam anemia hemolítica aguda, formação de trombos e coagulação intravascular http://dx

Molecular survey and genetic diversity of piroplasmids in equids from Midwestern Brazil

“…Despite the fact that B. caballi and T. equi have previously been detected in the Pantanal ecoregion (BARROS et al, 2015), this is the first study, to our knowledge, to show the prevalence of piroplasmids in Equidae in the entire state. Herein, we confirm that infection is widespread in the studied region, with higher prevalence of T. equi (25.91%) than B. caballi (2.74%) among equids.…”

“…The amplification conditions for B. caballi and T. equi were as follows: 40 cycles with an initial enzyme activation at 96 °C for 10 min, denaturation at 96 °C for 1 min, primer annealing at 60.5 °C for 1 min, extension at 72 °C for 1 min and final extension at 72 °C for 10 min. For all PCR assay, B. caballi and T. equi DNA samples obtained from naturally infected horse (BARROS et al, 2015) and ultra-pure sterile water were used as positive and negative controls, respectively. PCR product sizes were analyzed on 1.5% agarose gel stained with GelRed™ Nucleic Acid Gel Stain, and visualized in a ChemiDoc XRS system.…”

“…Despite the endemicity of piroplasmosis among equids in Brazil, studies involving the molecular detection of the parasite in carrier animals (HEIM et al, 2007;BALDANI et al, 2010;PECKLE et al, 2013;MACHADO et al, 2012;BARROS et al, 2015;FERREIRA et al, 2016;BRAGA et al, 2017) that have also examined genetic diversity (BRAGA et al, 2017;PECKLE et al, 2018) are scarce. The aim of this study is to survey the distribution of piroplasmid (T. equi and B. caballi) parasites using molecular methods in equids from the Mato Grosso state and evaluate the interspecific genetic diversity.…”

First Evidence of Ehrlichia minasensis Infection in Horses from Brazil

“…Sera samples were tested by indirect fluorescent antibody test (IFA) using E. canis Cuiabá # 1 strain antigens. The bacterium was cultured in DH82 cells as previously described [21]. Dilutions of the sera samples at 1:40 to 1:5120 were prepared in PBS (pH 7.2) and applied to the IFA slides containing the antigen previously fixed using Acetone.…”

“…Despite studies on Ehrlichia infection in equine being scarce in Brazil, seropositivity to Ehrlichia antigens in horses was observed in the States of Paraná [20] and Mato Grosso [21], and the bacterial DNA was also detected in horses of Paraná [8]. Subsequent molecular surveys in Brazil suggest that the Brazilian isolate of Ehrlichia detected in horses may potentially belong to the same group as that detected in Nicaragua [5,22].…”