Outbreak of avian aspergillosis in colonial-bred chicks (Isa Brown) in southern Rio Grande do Sul - case report

Zamboni, Rosimeri; Alberti, Taina dos Santos; Scheid, Haide Valeska; Venancio, Fabiano da Rosa; Brunner, Carolina Buss; Martins, O.A.; Raffi, Margarida Buss; Sallis, Eliza Simone Viégas

doi:10.1590/1678-4162-11878

Cited by 5 publications

(7 citation statements)

References 10 publications

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“…Conidiophores were short, smooth-walled, and had conical-shaped terminal vesicles in the case of A. fumigatus , with a dome-shaped vesicle and blue-green heads. Aspergillus niger showed large, globose, dark brown conidial heads, and conidiophores were smooth-walled, hyaline, or turned dark toward the vesicle after lactophenol cotton blue staining [ 16 , 17 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of Aspergilli from commercial chicken in selected areas of Bangladesh

Arafat¹,

Islam²,

Ahamed³

et al. 2022

J Adv Vet Anim Res

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Objectives: This study was designed to isolate, identify, and determine the prevalence of Aspergilli in commercial chicken in selected areas of Bangladesh. Materials and Methods: A total of 50 lung samples from suspected dead chickens, comprising broilers (n = 32) and layers (n = 18), aged between 5 days and 45 weeks, were collected from poultry farms located in the Gazipur district in Bangladesh. Fungi were primarily identified based on the colony morphology using potato dextrose agar (PDA). DNA was extracted from the suspected colonies. Aspegillus spp. was detected by genus-specific ASAP-1 and ASAP-2. Aspergillus spp. were then screened by polymerase chain reaction targeting Aspergillus flavus (FLA-1 and FLA-2), Aspergillus fumigatus (ASPU and Af3r), and Aspergillus niger (ASPU and Nilr). Results: The overall prevalence of Aspergillus spp. was 44% (n = 22/50; p < 0.05). Among the Aspergilli, A. flavus was detected in 10% (n = 5/50) of the samples. Similarly, A. fumigatus and A. niger were detected at 26% (n = 13/50) and 8% (n = 4/50) respectively. Three samples were associated with more than one fungus; two fungi (A. flavus and A. niger) were in two samples, and three fungi (A. flavus, A. fumigatus, and A. niger) were in one sample. Conclusion: Isolation and prevalence of Aspergillus spp. in commercial chicken were studied for the first time in Bangladesh.

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Section: Discussionmentioning

confidence: 99%

Molecular detection of Aspergilli from commercial chicken in selected areas of Bangladesh

Arafat¹,

Islam²,

Ahamed³

et al. 2022

J Adv Vet Anim Res

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“…A aspergilose aviária foi à afecção fúngica mais frequente observada nos casos analisados com 11/12 diagnósticos. Aspergilose é uma causa frequente de pneumonia e aerossaculite granulomatosa em aves, principalmente jovens, com maior importância econômica para o setor avicultura industrial (Arné et al, 2011;Dutta et al, 2017), sendo poucos os casos descritos em criações semi-intensivas ou extensivas (Zamboni et al, 2020). Os fatores predisponentes relacionados ao desenvolvimento e altas concentrações do fungo Aspergillus spp.…”

Section: Discussionunclassified

Estudo retrospectivo de doenças diagnosticadas em galinhas coloniais (Gallus gallus domesticus) na região sul do Rio Grande do Sul, em um período de 20 anos (2000 - 2020)

Zamboni

Alberti

Venancio

et al. 2021

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O objetivo desse estudo foi identificar e descrever as principais doenças que acometeram galinhas criadas em sistemas alternativos, encaminhadas ao Laboratório Regional de Diagnóstico da Faculdade de Veterinária da Universidade Federal de Pelotas (LRD/UFPel), entre os anos de 2000 e 2020. Foram revisados os protocolos de necropsia de galinhas domésticas encaminhadas ao LRD/UFPel, nos últimos 20 anos. Nesse período foram recebidas 565 amostras correspondentes a cadáveres e órgãos de galinhas domésticas, sendo 229 de criações alternativas. Dos 229 casos, 203 (88,64%) tinham diagnóstico conclusivo. As principais doenças diagnosticadas foram bacterianas com 79/203 (38,92%) casos, seguidas das parasitárias 59/203 (29,06%). Dentre as doenças bacterianas, as salmoneloses foram as mais frequente com 26/79 casos, sendo isolados 22/26 biovares Gallinarum e 4/26 Pullorum. Das doenças parasitárias, as parasitoses mistas foram as mais prevalentes com 37/59 casos, associadas principalmente ao nematódeo intestinal Ascaridia galli. As principais causas de morte nas aves desse estudo foram as parasitoses mistas, seguidas das salmoneloses aviárias (tifo aviário e pulorose). O sistema de criação e o manejo sanitário inadequado, tanto das aves quanto do ambiente, foram os principais fatores relacionados à ocorrência das doenças infecciosas diagnosticadas no LRD/UFPel.

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“…Згідно із дослідженнями R. Zamboni, захворювання діагностували у курчат від 5 до 10-добового віку, за повідомленнями інших авторів [6] гострий аспергільоз виявляють у курчат у віці від 3-х діб до 20 тижнів, що призводить до летальності 4,5-90 % птиці. Виникнення гострої форми аспергільозу виникає внаслідок потрапляння значної кількості конідій у дихальні шляхи, хронічна -за імуносупресії [7].…”

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“…Зараження відбувається інгаляційно, через потрапляння дрібних гідрофобних спор грибів (конідій) у дихальні шляхи птиці. Після потрапляння конідій на слизову оболонку дихальних шляхів (гортані, трахеї, повітросних мішків та парабронхів) вони починають розмножуватись прямим поділом, утворюючи гіфи які формують міцелій [6]. Запальний процес характеризується місцевою клітинною інфільтрацією, формуванням гігантських клітин і ексудативними явищами, утворенням вузликів або гранульом [5][6][7][8].…”

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“…Аспергільоз виникає внаслідок потрапляння значної кількості дрібних гідрофобних спор грибів (конідій) у дихальні шляхи [5,6,8,20]. Спори грибів проникають через дихальні шляхи та осідають на слизовій оболонці трахеї, в бронхах, легенях, зумовлюючи запальний процес [21].…”

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Conducting a comprehensive mycological study to prevent the occurrence of aspergillosis in chickens

Tyshkivska

Lyasota

Tyshkivska

et al. 2021

Nauk. vìsn. vet. med.

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Diseases of poultry aspergillosis are recorded in many poultry farms, the causes of the disease are the violation of the sanitary and hygienic conditions of hatcheries and poultry houses (unfavorable microclimate, affected feed and litter by microscopic fungi). To prevent the development of infection, it is necessary to control air pollution and equipment in hatcheries; determine the safety indicators of feed and water (the level of their mycological contamination) in poultry houses; monitor the status of feed storage facilities and water supply systems. According to the results of our research, in 15.1% of dead chickens at the age of 3 to 5 days and 6.6% at the age of 7-10 days, according to the results of mycological examination of pathological material, the diagnosis of aspergillosis was established. Aspergillus flavus (47%) Aspergillus fumigatus (34.5%), Aspergillus niger (10%) were isolated from the lungs of dead chickens. Macroscopically revealed edema and hyperemia of the lungs in chickens of 3-5 days of age. The formation of granulomas in the lungs and serous membranes was not detected, which may indicate an acute form of the pathological process. Chickens 7-10 days old showed a strong thickening of the air sacs, lungs with multiple spherical granulomas up to 2 mm in diameter. Spores of fungi entering the lungs cause a local inflammatory reaction - acute pneumonia. Histologically, in chickens of 3-5 days of age, a thickening of the parabronchial wall due to hyperplasia of local lymphoid formations (providing local immunity), signs of the development of interstitial pneumonia were revealed. Pathognomonic signs were found in the parenchyma - the formation of giant multinucleated cells, which is a characteristic sign of mycotic infections in poultry. The fusion of local pulmonary macrophages, histiocytes, into multinucleated cells is a prerequisite for the formation of granulomas, being a specific immune response in birds to the penetration of a pathogen. According to the development of the infectious process, caseous granulomas and fungal hyphae can be detected microscopically in the lungs. According to the results of histological examination, the formation of mycotic granulomas was found in the lung parenchyma in 7-10 days old chickens, which was accompanied by the formation of a zone of coagulation necrosis, in which the fungal hyphae were found. On the periphery, giant multinucleated epithelioid cells are located, the detection of which by histological examination is the basis for suspecting bird infection with microscopic fungi. Microscopic fungi Aspergillus flavus and Aspergillus fumigatus have been found in washes from hatchery ventilation cabinets, and Aspergillus flavus was found in the air from the chick sorting room and in washes from the feed mixer. Key words: aspergillosis, Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, micromycete, mold fungi, histiocytes, granulomas, phylaids, conidia, hyphae.

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Outbreak of avian aspergillosis in colonial-bred chicks (Isa Brown) in southern Rio Grande do Sul - case report

Cited by 5 publications

References 10 publications

Molecular detection of Aspergilli from commercial chicken in selected areas of Bangladesh

Molecular detection of Aspergilli from commercial chicken in selected areas of Bangladesh

Estudo retrospectivo de doenças diagnosticadas em galinhas coloniais (Gallus gallus domesticus) na região sul do Rio Grande do Sul, em um período de 20 anos (2000 - 2020)

Conducting a comprehensive mycological study to prevent the occurrence of aspergillosis in chickens

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