Detection of icaA, icaD, and bap genes and biofilm production in Staphylococcus aureus and non-aureus staphylococci isolated from subclinical and clinical bovine mastitis

Salina, A.; Guimarães, F. F.; Richini-Pereira, Virgínia Bodelão; Menozzi, Benedito Donizete; Rall, Vera Lúcia Mores; Langoni, Hélio

doi:10.1590/1678-4162-11284

Cited by 8 publications

(4 citation statements)

References 11 publications

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“…The icaABCD operon is the most studied Staphylococcus biofilm forming genes and it is most frequently reported in mastitis isolates highlighting their potential to form biofilm 30 . In the current study, the icaC gene was the most prevalent in clinical and subclinical isolates, in contrast to a previous report of which icaA and icaD are the most prevalent 31 . Also, the finding of this study observed that coagulase negative Staphylococcus (CoNS) and S. aureus possessed the icaA and/or icaD gene in contrast with a previous findings that the icaA was only observed in CoNS strain while the icaD was found both in CoNS and S. aureus .…”

Section: Discussioncontrasting

confidence: 99%

Genomic comparisons and phylogenetic analysis of mastitis-related staphylococci with a focus on adhesion, biofilm, and related regulatory genes

Pizauro

Almeida

Silva

et al. 2021

Sci Rep

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Mastitis is a common and costly disease on dairy farms, commonly caused by Staphylococcus spp. though the various species are associated with different clinical outcomes. In the current study, we performed genomic analyses to determine the prevalence of adhesion, biofilm, and related regulatory genes in 478 staphylococcal species isolated from clinical and subclinical mastitis cases deposited in public databases. The most prevalent adhesin genes (ebpS, atl, pls, sasH and sasF) were found in both clinical and subclinical isolates. However, the ebpS gene was absent in subclinical isolates of Staphylococcus arlettae, S. succinus, S. sciuri, S. equorun, S. galinarum, and S. saprophyticus. In contrast, the coa, eap, emp, efb, and vWbp genes were present more frequently in clinical (vs. subclincal) mastitis isolates and were highly correlated with the presence of the biofim operon (icaABCD) and its transcriptional regulator, icaR. Co-phylogenetic analyses suggested that many of these adhesins, biofilm, and associated regulatory genes could have been horizontally disseminated between clinical and subclinical isolates. Our results further suggest that several adhesins, biofilm, and related regulatory genes, which have been overlooked in previous studies, may be of use for virulence profiling of mastitis-related Staphylococcus strains or as potential targets for vaccine development.

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Section: Discussioncontrasting

confidence: 99%

Genomic comparisons and phylogenetic analysis of mastitis-related staphylococci with a focus on adhesion, biofilm, and related regulatory genes

Pizauro

Almeida

Silva

et al. 2021

Sci Rep

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“…The icaADBC locus contains the most studied Staphylococcus bio lm forming genes and 26 it is most frequently reported in mastitis isolates. In the current study, the icaC gene was the most prevalent in subclinical isolates, in contrast to previous report of which icaA and icaD are the most prevalent 27 . The most prevalent bio lm regulatory genes were rbf, tcaR, sarA and sigB.…”

Section: Discussioncontrasting

confidence: 99%

Genome Comparison and Phylogenetic Analysis of Mastitis-Related Staphylococci with a Focus on Adhesion, Biofilm, and Regulatory-Related Genes

Pizauro

Almeida

Silva

et al. 2021

Preprint

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Bovine mastitis is the costliest diseases on dairy farms and is caused by different Staphylococcus species. However, staphylococci associated with clinical mastitis infections are different from subclinical ones, indicating a complex mechanism related to bovine mastitis pathogenesis. Here, we performed genomic analyses to determine the prevalence of adhesion, biofilm, and regulatory genes in 478 staphylococcal spp. associated with clinical and subclinical mastitis deposited in public databases. The most prevalent adhesin genes were the ebpS, atl, pls, sasH and sasF genes found in both clinical and subclinical isolates. However, the ebpS gene is absent in subclinical isolates of Staphylococcus arlettae, S. succinus, S. sciuri, S. equorun, S. galinarum, and S. saprophyticus. In constrast, the coa, eap, emp, efb, and vWbp genes were present more frequently in clinical mastitis isolates and highly correlated with the presence of the icaABCD and icaR biofilm genes. We also revealed that many adhesins, biofilm, and associated regulatory genes were potentially horizontally disseminated between clinical and subclinical isolates. Taken together, our results indicate that several adhesins, biofilm, and regulatory-related genes have been overlooked in previous studies and that these virulence factors may arise in staphylococcal species not generally associated with clinical mastitis by horizontal gene transfer.

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“…Singh et al (2013) also detected 98% staphylococci isolates able to produce biofilm whereas just 77.9% and 75.7% presented icaA and icaD genes, respectively. A recent study, developed by Salina et al (2020), there was no association between the presence of icaAD genes and biofilm formation, confirming that this correlation is not clear, and needs to be better understand.…”

Section: Discussionmentioning

confidence: 93%

Expression of icaA and icaD genes in biofilm formation in Staphylococcus aureus isolates from bovine subclinical mastitis

Marques

Santos

et al. 2021

Pesq. Vet. Bras.

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Staphylococcus spp. plays a significant role in the etiology of bovine mastitis. Staphylococcus aureus is considered the most important species due to the high prevalence and the difficulty of in vivo treatment that is related to the expression of virulence factors and biofilm formation. This study aimed to detect the phenotypic expression of the biofilm formation in 20 S. aureus isolated from bovine mastitis and to evaluate the expression and regulation of genes involved in its production. MALDI-TOF and phenogenotypic identification assays were performed to characterize the isolates. The phenotypic biofilm production and the presence of icaA and icaD and bap genes were evaluated. The Agr system was typified (agr I, agr II, agr III and agr IV) and its regulator (agr RNAIII) was detected. Furtherly, Real-time PCR (qPCR) was performed at chosen times to quantify the expression of icaA, icaD and hld genes in three selected isolates. All 20 strains were biofilm producers and most presented icaA and icaD genes. Only one isolate presented the bap gene. The agr gene type II showed a prevalence of 70%. Transcriptional analysis revealed increased expression of ica genes at eight hours of growth. These results confirm that polysaccharides production mediated by the icaADBC operon genes is an essential mechanism to the biofilm formation and contributes to the early stages of bacterial growth.

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Detection of icaA, icaD, and bap genes and biofilm production in Staphylococcus aureus and non-aureus staphylococci isolated from subclinical and clinical bovine mastitis

Cited by 8 publications

References 11 publications

Genomic comparisons and phylogenetic analysis of mastitis-related staphylococci with a focus on adhesion, biofilm, and related regulatory genes

Genomic comparisons and phylogenetic analysis of mastitis-related staphylococci with a focus on adhesion, biofilm, and related regulatory genes

Genome Comparison and Phylogenetic Analysis of Mastitis-Related Staphylococci with a Focus on Adhesion, Biofilm, and Regulatory-Related Genes

Expression of icaA and icaD genes in biofilm formation in Staphylococcus aureus isolates from bovine subclinical mastitis

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