Effects of transrectal medicinal ozone in horses - clinical and laboratory aspects

Abstract

Ozone (O3) therapy has been used for medical procedures for centuries; however, there are no extensive studies on its utilization in horses. This study aimed to evaluate the application of transrectal O3 on horses by physical and laboratorial evaluation, and production of reactive oxygen species (ROS). Sixteen healthy horses were separated in two groups: a control group (CG) and a group treated with O3 (TG). The TG animals received 1L of an oxygen and O3 mixture transrectally. The initial dose was 10µg/ml for the first two applications, 15μg/ml for the following two applications, and 20μg/ml for the next six applications. The CG animals received 1L of oxygen transrectally. In TG animals no variations in the physical examination were detected; furthermore, TG animals did not exhibit changes in biochemical evaluation results, fibrinogen concentrations, or ROS production. TG animals had increased red blood cell counts, hemoglobin concentrations, and packet cell volume values in comparison to the baseline and CG values. We could infer that O3 affected the red blood cell counts and improved rhetological properties of the blood. The transrectal application of O3 in horses is safe and can indirectly improve the oxygenation and metabolism of tissues.

Ozone and its derivatives in veterinary medicine: A careful appraisal

“…Morever, gaseous ozone is also used by rectal insufflation to achieve a systemic effect ( Jaramillo et al., 2020 ). While the human colon is sacculated, canine colon does not have sacculation.…”

“… Jaramillo et al. (2020) divided 16 healthy horses into two groups: a control group, where the horses received only oxygen; and the treated group, where the horses received 1L of gaseous intrarectal ozone three times a week.…”

Multimodal therapy for treatment of equine back pain: a report of 15 cases

“…The objective of ozone therapy is to control acute, adequate, and transitional oxidative stress without causing chronic oxidative stress. Repeated small oxidative shocks activate and stimulate the antioxidant system, consequently creating resistance to oxidative stress by inducing the antioxidant system ( Jaramillo et al, 2020 ).…”

Uso de ozonioterapia no tratamento de feridas em cão: Relato de caso

“…Tais resultados podem auxiliar na cicatrização das feridas, bem como relatado no presente caso, no qual após o início do tratamento o animal apresentou melhora expressiva a cada sessão, utilizando-se tratamentos alopáticos e integrativos que culminaram na redução das feridas. Tusat et al (2017) apontam o uso de ozônio como um excelente anti-inflamatório, corroborando com Jaramillo et al (2020) que afirmam a ocorrência do efeito anti-inflamatório pela diminuição do nível de determinados mediadores pró-inflamatórios, oxidando mediadores de dor e, consequentemente, melhorando a microcirculação local com melhor oxigenação tecidual. Gonçalves et al (2020) propuseram um estudo envolvendo Leishmaniose Visceral canina, realizando o tratamento com a ozonioterapia de diversas formas, sendo elas por via retal, peri-lesional e autohemoterapia, observando-se melhora clínica do paciente, com diminuição dos sinais clínicos, como dor e febre, e diminuição das demais alterações hematológicas e tegumentares.…”