2020
DOI: 10.1590/1678-4162-11140
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Abstract: RESUMO O objetivo foi avaliar protocolos de maturação in vitro (MIV) para oócitos de cutias, seguida de fertilização in vitro (FIV) e ativação partenogenética (AP). Os oócitos imaturos (CCOs) foram obtidos por fatiamento do ovário, após OSH, e submetidos a três grupos: MAT - 16 (16 horas de maturação), MAT - 20 (20 horas de maturação) e MAT - 24 (24 horas de maturação), em incubadora de cultivo a 38,8°C, com atmosfera de 5% de CO2 e 95% de umidade relativa. A maturação foi analisada pela presença do primeiro c… Show more

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“…In general, IVM success depends on suitable temperature (Wang et al, 2009), time (Ferraz et al, 2020), and composition of the medium (Lin et al, 2011). Considering that culture systems can decrease oocyte quality, using medium supplements that guarantee meiotic competence is a crucial step in achieving good rates of initial embryonic development (Borges et al, 2020).…”
Section: Introductionmentioning
confidence: 99%
“…In general, IVM success depends on suitable temperature (Wang et al, 2009), time (Ferraz et al, 2020), and composition of the medium (Lin et al, 2011). Considering that culture systems can decrease oocyte quality, using medium supplements that guarantee meiotic competence is a crucial step in achieving good rates of initial embryonic development (Borges et al, 2020).…”
Section: Introductionmentioning
confidence: 99%