Salmonella Minnesota de origem avícola apresenta fatores de virulência e risco potencial aos humanos

Melo, Roberta Torres de; Resende, Anselmo; Mendonça, Eliane Pereira; Nalevaiko, Priscila Christen; Monteiro, Guilherme Paz; Buiatte, Ana Beatriz Garcez; Rossi, Daise Aparecida

doi:10.1590/1678-4162-10884

Cited by 7 publications

(9 citation statements)

References 20 publications

(6 reference statements)

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“…In addition to the possibility of the same supplier, according to a set of failures in hygienic conditions, such as The presence of Salmonella spp. in poultry meat was also observed in the studies done by (BAPTISTA et al, 2018;MELO et al, 2020;YIN et al, 2021). However, our findings differ from these studies due to the higher prevalence of Salmonella spp isolates.…”

Section: Discussioncontrasting

confidence: 57%

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Salmonella spp. virulent and resistant multidrug recovered from chicken carcasses in Brazil

Silva¹,

Almeida²,

Medeiros³

et al. 2023

Revista Científica Multidisciplinar Núcleo do Conhecimento

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The aim of this study was to evaluate the biofilm production, the susceptibility profile and the detection of resistance genes present in Salmonella spp isolates from fresh chicken carcasses sold in a Brazilian metropolis. From a total of 61 samples of fresh poultry carcasses, 21 were positive for the presence of Salmonella spp. Regarding the antimicrobial susceptibility test, (13/21) isolates tested were resistant to at least one antibiotic, corresponding to 61.9%, and 38% (08/21) were Resistant to Multiple Drugs. At least two resistance genes were identified in all isolates, especially the genes related to β-lactamases and Quinolones resistance. It was also observed that some Salmonella spp isolates showed identical genetic patterns. And all 21 isolates were able to form biofilm. The identification of Salmonella spp. biofilm forming and carrying different β-lactamase genes and determinants of resistance to quinolones demonstrates the capacity of these bacteria to accumulate various mechanisms of virulence and resistance to antimicrobials. Therefore, the spread of different clonal groups of Salmonella spp. MDR in poultry meat carcasses expressed in this attest to the need for effective controls to contain this microorganism, which besides being a risk to public health, is also responsible for considerable economic losses.

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Section: Discussioncontrasting

confidence: 57%

“…Hence, the occurrence of Salmonella MDR strains in food animal origin is increasing in Brazil (BAPTISTA et al, 2018;MELO et al, 2020;VOSS-RECH et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Salmonella spp. virulent and resistant multidrug recovered from chicken carcasses in Brazil

Silva¹,

Almeida²,

Medeiros³

et al. 2023

Revista Científica Multidisciplinar Núcleo do Conhecimento

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“…Similar studies with different Salmonella serovars have shown the presence of the agf A gene in over 91.4%, the lfp A gene in 80.63%, and the three genes together in more than 73.34% of strains isolated from birds. The frequency of virulence determinants and the establishment of genetic profiles of isolates help to determine effective control protocols and prevention measures in the industry [ 15 , 23 , 24 ].…”

Section: Resultsmentioning

confidence: 99%

“…Six BFI profiles were identified (A–F), the most prevalent being those classified as nonexistent at the three temperatures (9/20–45.0%; Table 1 ). The BFI classification for this serovar is strain-dependent, and there are probably other factors determining this variability, such as the presence and expression of genes linked to biofilm formation ( agf A, lpf A, and lux S) that encode aggregative fimbriae and quorum sensing systems, whose functions favor the biofilm formation [ 24 ]. All strains had at least one of these genes, indicating the potential for sessile life that was expressed differently and according to external factors and the expression of this potential.…”

Section: Resultsmentioning

confidence: 99%

Salmonella enterica Serovar Minnesota Biofilms, Susceptibility to Biocides, and Molecular Characterization

et al. 2021

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The presence of virulence genes, phylogenetic relationships, biofilm formation index (BFI), and ultrastructure in S. Minnesota at different temperatures (4, 25, and 36 °C) were analyzed. In addition, the ability of biocidal agents (chlorhexidine1%, sodium hypochlorite 1%, and peracetic acid 0.8%) to inhibit biofilms formed by 20 strains isolated from broiler slaughter plants from two Brazilian companies in 2009, 2010, and 2014 was determined. The presence of specific genes was evaluated by PCR and phylogeny between strains by pulsed-field gel electrophoresis. The BFI was determined using tryptone soy broth with 5% of chicken juice, and its structure was observed by scanning electron microscopy. The presence of specific genes indicated that S. Minnesota has the potential to cause disease in humans, adapting to adverse conditions. Temperatures of 25 and 36 °C favored biofilm formation, although at 4 °C, there was still biomass that could contaminate the final product. Tolerance to all biocides was identified in 12/20 (60%), representing a real risk of adaptation mechanisms development, especially regarding to resistance to sodium hypochlorite. Phylogenetic analysis indicated cross-contamination and spread among companies, which was probably related to biofilms formation. Results show the necessity of attention to this serovar considering its resistance to sodium hypochlorite, including the need for rigorous control, adopting low temperatures to prevent biofilms formation in the poultry industry.

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“…The exception was pulsotype IV, whose strains had all the common epidemiological and molecular characteristics. In this case, the local maintenance of the microorganism in different samples can be linked to cross contamination (Melo et al, 2020).…”

Section: Genetic Similarity Between Strainsmentioning

confidence: 99%

Molecular Characterization and Survive Abilities of Salmonella Heidelberg Strains of Poultry Origin in Brazil

et al. 2021

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The aim of the study was to evaluate the genotypic and phenotypic characteristics of 20 strains of S. Heidelberg (SH) isolated from broilers produced in southern Brazil. The similarity and presence of genetic determinants linked to virulence, antimicrobial resistance, biofilm formation, and in silico-predicted metabolic interactions revealed this serovar as a threat to public health. The presence of the ompC, invA, sodC, avrA, lpfA, and agfA genes was detected in 100% of the strains and the luxS gene in 70% of them. None of the strains carries the blaSHV, mcr-1, qnrA, qnrB, and qnrS genes. All strains showed a multidrug-resistant profile to at least three non-β-lactam drugs, which include colistin, sulfamethoxazole, and tetracycline. Resistance to penicillin, ceftriaxone (90%), meropenem (25%), and cefoxitin (25%) were associated with the presence of blaCTX–M and blaCMY–2 genes. Biofilm formation reached a mature stage at 25 and 37°C, especially with chicken juice (CJ) addition. The sodium hypochlorite 1% was the least efficient in controlling the sessile cells. Genomic analysis of two strains identified more than 100 virulence genes and the presence of resistance to 24 classes of antibiotics correlated to phenotypic tests. Protein-protein interaction (PPI) prediction shows two metabolic pathways correlation with biofilm formation. Virulence, resistance, and biofilm determinants must be constant monitoring in SH, due to the possibility of occurring infections extremely difficult to cure and due risk of the maintenance of the bacterium in production environments.

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Salmonella Minnesota de origem avícola apresenta fatores de virulência e risco potencial aos humanos

Cited by 7 publications

References 20 publications

Salmonella spp. virulent and resistant multidrug recovered from chicken carcasses in Brazil

Salmonella spp. virulent and resistant multidrug recovered from chicken carcasses in Brazil

Salmonella enterica Serovar Minnesota Biofilms, Susceptibility to Biocides, and Molecular Characterization

Molecular Characterization and Survive Abilities of Salmonella Heidelberg Strains of Poultry Origin in Brazil

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