Immunophenotyping, plasticity tests and nanotagging of stem cells derived from adipose tissue of wild rodent agouti (Dasyprocta prymnolopha)

Abstract

There is a growing interest in the study of unspecialized mesenchymal stem cells, for there are still some discussions about their in vitro behavior. Regenerative medicine is a science undergoing improvement which develops treatments as cell therapy using somatic stem cells. In several studies, adipose tissue is presented as a source of multipotent adult cells that has several advantages over other tissue sources. This study aimed to characterize and evaluate the tagging of mesenchymal stem cells from the agoutis adipose tissue (Dasyprocta prymonolopha), with fluorescent intracytoplasmic nanocrystals. Fibroblast cells were observed, plastic adherent, with extended self-renewal, ability to form colonies, multipotency by differentiation into three lineages, population CD90 + and CD45 - expression, which issued high red fluorescence after the tagging with fluorescent nanocrystals by different paths and cryopreserved for future use. It is possible to conclude that mesenchymal stem cells from agouti adipose tissue have biological characteristics and in vitro behavior that demonstrate its potential for use in clinical tests.

Renal Progenitor Cells Have Higher Genetic Stability and Lower Oxidative Stress than Mesenchymal Stem Cells during In Vitro Expansion

“…Similarly, necrosis rates of the RPCsu lineage were also lower than the apoptosis rates, at first passage, with elevation after the sixth day of cultivation. Similar results have been described [23,33,34]. The increase in these rates is probably due to exhaustion of the culture medium due to the large cellular confluence, inducing necrosis [33].…”

“…Nevertheless, MSCBMsu showed a logarithmic increase over nine days before showing a reduction in cell concentration (Figure 1). This characteristic is not uncommon among mesenchymal stem cell lines, and studies with bone marrow, adipose tissue, and dental pulp lineages have commonly demonstrated similar evidence [33][34][35]. This observation raises the possibility that this group of somatic stem cells may present relevant metabolic adaptability.…”

Characterization and plasticity of wharton's jelly mesenchymal stem cells of goat

“…Nevertheless, WJ-MSCs showed a logarithmic increase over nine days, with no Plateau Phase display, before showing a reduction in cell concentration (Figure 2). This characteristic is not uncommon among mesenchymal stem cell lines, and studies with bone marrow, adipose tissue, and dental pulp lineages have commonly demonstrated similar evidence (Argôlo Neto et al 2016;Rocha et al 2019;Soares et al 2019). This observation raises the possibility that this group of somatic stem cells may present relevant metabolic adaptability.…”