2024
DOI: 10.1590/1519-6984.253514
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Abstract: The objective of the current study was to investigate the synergistic impact of α-Tocopherol and α-Linolenic acid (100 µM) on IVM and IVC of Nili Ravi buffalo oocytes. Oocytes were obtained from the ovaries of slaughtered buffaloes within two hours after slaughter and brought to laboratory. Buffalo cumulus oocyte complexes were placed randomly in the five experimental groups included; GROUP 1: Maturation media (MM) + 100 µM ALA (control), GROUP 2: MM + 100 µM ALA + 50μM α-Tocopherol, GROUP 3: MM + 100 µM ALA +… Show more

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“…Another study found that supplementing IVM with α-tocopherol had no effect on the rate of metaphase II (MII), GVBD, or cleavage when incubated in 5% O 2 at 39°C for 24 hours [92] . According to many studies, α-tocopherol supplementation has a deleterious influence on the capacity of bovine oocytes to reach MII, increasing the number of apoptotic and necrotic cumulus cells in bovine COC [93,94] . At a dose of 100 mmol/L, this antioxidant improved the viability of cultivated GC [95] .…”
Section: Introductionmentioning
confidence: 99%
“…Another study found that supplementing IVM with α-tocopherol had no effect on the rate of metaphase II (MII), GVBD, or cleavage when incubated in 5% O 2 at 39°C for 24 hours [92] . According to many studies, α-tocopherol supplementation has a deleterious influence on the capacity of bovine oocytes to reach MII, increasing the number of apoptotic and necrotic cumulus cells in bovine COC [93,94] . At a dose of 100 mmol/L, this antioxidant improved the viability of cultivated GC [95] .…”
Section: Introductionmentioning
confidence: 99%